Skip to main content
SpringerLink
Log in

Infectious bursal disease vaccine ameliorates velogenic Newcastle disease virus infection in immunopotentiated chickens

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

In the present study, the potential immunoprotective role of infectious bursal disease (IBD) vaccine on immunopotentiated chickens challenged with velogenic viscerotropic Newcastle disease virus (vvNDV) genotype VIId was investigated. The chickens were divided into five groups vaccinated with NDV vaccine, but group (G1) was kept as control unvaccinated birds. The G2 and G4 were immunopotentiated using mannan oligosaccharides (MOS) while G3 and G5 received MOS and IBD vaccine. The G1, G2, and G3 were infected with vvNDV while G4 and G5 served as uninfected birds. The humoral response to NDV vaccine was measured by hemagglutination inhibition test while cellular response was estimated by chicken interleukin-2 ELISA, total and differential leucocytic count, bursa body weight index, and histopathological examination of the bursae. The results revealed that the immunopotentiated groups possessed a good cellular and humoral response to vvNDV although the severity of histopathological lesions was variable. The G3 had the best cellular and humoral response, and IBD vaccine enhanced the immune response against the infection with vvNDV. Histopathologically, the typical bursal lesions of NDV were observed in G1 such as lymphocytic depletion, acute inflammatory reaction, and degenerative changes of the bursal follicles. In contrast, such histopathological changes in the immunopotentiated groups were less evident both in severity and prevalence, while the immune reactive lymphoid hyperplasia was a prominent finding in G4 and G5. In conclusion, the immunopotentiation of the chickens as well as NDV and IBD vaccination improved the bird immunity and diminished the immunosuppressive effect of the vvNDV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Aldous EW, Alexander DJ (2001) Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1). Avian Pathol 30:117–128

    Article  CAS  PubMed  Google Scholar 

  • Aldous EW, Mynn JK, Banks J, Alexander DJ (2003) A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene. Avian Pathol 32:239–256

    Article  CAS  PubMed  Google Scholar 

  • Alexander DJ (1988) Newcastle disease. Methods of spread. In: Alexander DJ (ed) Newcastle disease. Kluwer Academic Publishers, Boston, pp 256–272

    Chapter  Google Scholar 

  • Alexander DJ (1991) Newcastle disease and other paramyxovirus infection. In: Calnek BW (ed) Diseases of poultry, 9th edn. Iowa State University Press, Ames, pp 495–519

    Google Scholar 

  • Alexander DJ (1997) Newcastle disease and other Paramyxoviridae infections. In: Calnek BW, Barnes HJ, Beard CW, McDougald L, Saif YM (eds) Diseases of poultry, 10th edn. Iowa State University Press, Ames, Iowa, pp 541–570

    Google Scholar 

  • Blachère NE, Morris HK, Braun D, Saklani H, Di Santo JP, Darnell RB, Albert ML (2006) IL-2 is required for the activation of memory CD8+ T cells via antigen cross-presentation. J Immunol 176:7288–7300

    Article  PubMed  Google Scholar 

  • Bogoyavlenskiy A, Berezin V, Prilipov A, Usachev E, Lyapina O, Levandovskaya S, Korotetskiy I, Tolmacheva V, Makhmudova N, Khudyakova S, Tustikbaeva G, Zaitseva I, Omirtaeva E, Ermakova O, Daulbaeva K, Asanova S, Kydyrmanov A, Sayatov M, King D (2005) Molecular characterization of virulent Newcastle disease virus isolates from chickens during the 1998 NDV outbreak in Kazakhstan. Virus Genes 31:13–20

    Article  CAS  PubMed  Google Scholar 

  • Brown C, King DJ, Seal B (1999) Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence. Vet Pathol 36:125–132

    Article  CAS  PubMed  Google Scholar 

  • Cannon MJ, Russell PH (1986) Secondary in vitro stimulation of specific cytotoxic cells to Newcastle disease virus in chickens. Avian Pathol 15:731–740

    Article  CAS  PubMed  Google Scholar 

  • Cattoli G, Leonardo S, Calogero T, Corrie B (2011) Newcastle disease: a review of field recognition and current methods of laboratory detection. J Vet Diagn Investig 23:637–656

    Article  Google Scholar 

  • Chen HL, Li DF, Chang BY, Gong LM, Dai JG, Yi GF (2003) Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers. Poult Sci 82:364–370

    Article  CAS  PubMed  Google Scholar 

  • Ezeokoli CD, Ityondo EA, Nwannenna AI, Umoh JU (1990) Immunosuppression and histopathological changes in the bursa of Fabricius associated with infectious bursal disease vaccination in chicken. Comp Immunol Microbiol Infect Dis 13:181–188

    Article  CAS  PubMed  Google Scholar 

  • Feau S, Arens R, Togher S, Schoenberger SP (2011) Autocrine IL-2 is required for secondary population expansion of CD8 (+) memory T cells. Nat Immunol 12:908–913

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Harrison L, Brown C, Afonso C, Zhang J, Susta L (2011) Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of Newcastle disease virus of varying virulence. J Comp Pathol 145:327–335

    Article  CAS  PubMed  Google Scholar 

  • Ingrao F, Rauw F, Lambrecht B, van den Berg T (2013) Infectious Bursal Disease: a complex host-pathogen interaction. Dev Comp Immunol 41:429–438

    Article  CAS  PubMed  Google Scholar 

  • Jeurissen SH, Janse EM, Lehrbach PR, Haddad EE, Avakian A, Whitfill CE (1998) The working mechanism of an immune complex vaccine that protects chickens against infectious bursal disease. Immunology 95:494–500

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kapczynski DR, Afonso CL, Miller PJ (2013) Immune responses of poultry to Newcastle disease virus. Dev Comp Immunol 41:447–453

    Article  CAS  PubMed  Google Scholar 

  • Kim IJ, Gagic M, Sharma JM (1999) Recovery of antibody-producing ability and lymphocyte repopulation of bursal follicles in chickens exposed to infectious bursal disease virus. Avian Dis 43:401–413

    Article  CAS  PubMed  Google Scholar 

  • Kim IJ, You SK, Kim H, Yeh HY, Sharma JM (2000) Characteristics of bursal T lymphocytes induced by infectious bursal disease virus. J Virol 74:8884–8892

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Marangon S, Busani L (2006) The use of vaccination in poultry production. Rev Sci Tech Off Int Epiz 26:265–274

    Article  Google Scholar 

  • Mazariegos LA, Lukert PD, Brown J (1990) Pathogenicity and immunosuppressive properties of infectious bursal disease “intermediate” strains. Avian Dis 34:203–208

    Article  CAS  PubMed  Google Scholar 

  • Merz DC, Scheid A, Choppin PW (1981) Immunological studies of the functions of paramyxovirus glycoproteins. Virology 109:94–105

    Article  CAS  PubMed  Google Scholar 

  • Mishra S, Kataria JM, Verma KC, Sah RL (2000) Response of chickens to infection with Newcastle disease virus isolated from a guinea fowl. Trop Anim Health Prod 32:277–284

    Article  CAS  PubMed  Google Scholar 

  • Mitchell DM, Ravkov EV, Williams MA (2010) Distinct roles for IL-2 and IL-15 in the differentiation and survival of CD8+ effector and memory T cells. J Immunol 184:6719–6730

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Munir M, Muhammad A, Muhammad TK, Siamak Z, Mikael B (2012) Genomic and biological characterization of a velogenic Newcastle disease virus isolated from a healthy backyard poultry flock in 2010. Virol J 9:1–11

    Article  Google Scholar 

  • Muskett JC, Hopkins IG, Edwards KR, Thornton DH (1979) Comparison of two infectious bursal disease vaccine strains: efficacy and potential hazards in susceptible and maternally immune birds. Vet Rec 104:332–334

    Article  CAS  PubMed  Google Scholar 

  • Nabila O, Sultan S, Ahmed IA, Ibrahim RS, Sabra M (2014) Isolation and pathotyping of Newcastle disease viruses from field outbreaks among chickens in the southern part of Egypt 2011-2012. Global Veterinaria 12:237–243

    Google Scholar 

  • Nasser M, Lohr JE, Mebratu GY, Zessin KH, Baumann MP, Ademe Z (2000) Oral Newcastle disease vaccination trials in Ethiopia. Avian Pathol 29:27–34

    Article  CAS  PubMed  Google Scholar 

  • Njagi LW, Nyaga PN, Bebora LC, Mbuthia PG, Minga UM (2012) Effect of immunosuppression on Newcastle disease virus persistence in ducks with different immune status. ISRN Vet Sci. doi:10.5402/2012/253809

    PubMed  PubMed Central  Google Scholar 

  • OIE (2012) Manual of diagnostic tests and vaccines for terrestrial animals, 7th edn. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.14_NEWCASTLE_DIS.pdf

  • Olah I, Glick B (1992) Follicle-associated epithelium and medullary epithelial tissue of the bursa of fabricius are two different compartments. Anat Rec 233:577–587

    Article  CAS  PubMed  Google Scholar 

  • Parede L, Young PL (1990) The pathogenesis of velogenic Newcastle disease virus infection of chickens of different ages and different levels of immunity. Avian Dis 34:803–808

    Article  CAS  PubMed  Google Scholar 

  • Rasoli M, Yeap SK, Tan SW, Moeini H, Ideris A, Bejo MH, Alitheen NB, Kaiser P, Omar AR (2014) Alteration in lymphocyte responses, cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus. Comp Immunol Microbiol Infect Dis 37:11–21

    Article  PubMed  Google Scholar 

  • Rautenschlein S, Yeh HY, Njenga MK, Sharma JM (2002) Role of intrabursal T cells in infectious bursal disease virus (IBDV) infection: T cells promote viral clearance but delay follicular recovery. Arch Virol 147:285–304

    Article  CAS  PubMed  Google Scholar 

  • Ravindra PV, Tiwari AK, Ratta B, Bais MV, Chaturvedi U, Palia SK, Sharma B, Chauhan RS (2009) Time course of Newcastle disease virus-induced apoptotic pathways. Virus Res 144:350–354

    Article  CAS  PubMed  Google Scholar 

  • Reed LJ, Muench LH (1938) A simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497

    Google Scholar 

  • Reynolds DL, Maraqa AD (2000) Protective immunity against Newcastle disease: the role of cell-mediated immunity. Avian Dis 44:145–154

    Article  CAS  PubMed  Google Scholar 

  • Schauenstein K, Krömer G, Fässler R, Wick G (1987) Implications of IL-2 in normal and disturbed immune functions in the chicken. Prog Clin Biol Res 233:69–77

    CAS  PubMed  Google Scholar 

  • Seal BS, King DJ, Sellers HS (2000) The avian response to Newcastle disease virus. Dev Comp Immunol 24:257–268

    Article  CAS  PubMed  Google Scholar 

  • Sijtsma SR, West CE, Rombout JH, Van der Zijpp AJ (1989) Effect of Newcastle disease virus infection on vitamin A metabolism in chickens. J Nutr 119:940–947

    CAS  PubMed  Google Scholar 

  • Susta L, Miller PJ, Afonso CL, Brown CC (2011) Clinicopathological characterization in poultry of three strains of Newcastle disease virus isolated from recent outbreaks. Vet Pathol 48:349–360

    Article  CAS  PubMed  Google Scholar 

  • Wakamatsu N, King DJ, Kapczynski DR, Seal BS, Brown CC (2006) Experimental pathogenesis for chickens, turkeys and pigeons of exotic Newcastle disease virus from an outbreak in California during 2002-2003. Vet Pathol 43:925–933

    Article  CAS  PubMed  Google Scholar 

  • Zou XT, Qiao XJ, Xu ZR (2006) Effect of beta-mannanase (Hemicell) on growth performance and immunity of broilers. Poult Sci 85:2176–2179

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology (Virology), Faculty of Veterinary Medicine, South Valley University, 83523, Qena, Egypt

    Serageldeen Sultan

  2. Department of Poultry Diseases, Faculty of Veterinary Medicine, South Valley University, 83523, Qena, Egypt

    Nabila Osman, Ahmed I. Ahmed & Rabab A. M. Abdallah

  3. Department of Poultry Diseases, Faculty of Veterinary Medicine, Assiut University, 71515, Assiut, Egypt

    Moemen A. Mohamed

  4. Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt

    Marwa Faraouk & Mokhtar Taha

Authors
  1. Serageldeen Sultan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nabila Osman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Moemen A. Mohamed
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ahmed I. Ahmed
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rabab A. M. Abdallah
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Marwa Faraouk
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mokhtar Taha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Serageldeen Sultan or Mokhtar Taha.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sultan, S., Osman, N., Mohamed, M.A. et al. Infectious bursal disease vaccine ameliorates velogenic Newcastle disease virus infection in immunopotentiated chickens. Comp Clin Pathol 25, 91–100 (2016). https://doi.org/10.1007/s00580-015-2145-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-015-2145-5

Keywords

Search

Navigation