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Lipid peroxidation and decrease on the activities of antioxidant enzymes in experimental infection by Babesia bovis in cattle

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Abstract

Babesia bovis is one of the causative agents of bovine babesiosis, a disease with high morbidity and mortality in susceptible populations. The aim of this study was to evaluate the occurrence of oxidative stress in cattle experimentally infected with attenuated B. bovis. For that, eight healthy cattle were used and divided into two groups: animals infected with B. bovis (n = 4; group A) and non-infected animals (n = 4; group B). Blood samples of all animals were collected at 0, 7, 11, and 15 days post-infection (DPI) for red blood cells (RBCs) count, and measurement of thiobarbituric acid reactive substance (TBARS) levels in serum (lipid peroxidation) and activity of antioxidant enzymes in whole blood (catalase (CAT) and superoxide dismutase (SOD)). The parasitemia was determined by blood smear evaluation and conventional PCR for B. bovis. Blood smears were negative throughout the experiment; however, infection was confirmed by PCR positive for B. bovis at 15 DPI. A slight reduction on RBC count was observed in cattle of group A at 11 and 15 DPI (P < 0.05). The same animals showed an increased level of TBARS (P < 0.05) at 11 DPI, suggesting lipid peroxidation, while the activities of CAT and SOD decreased (P < 0.05) at 7 and 15 DPI, respectively. Our data support the occurrence of an oxidative/antioxidant imbalance in cattle infected with B. bovis.

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Acknowledgments

This work was supported by the Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) and Fundação Estadual de Pesquisa Agropecuária (FEPAGRO).

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Authors and Affiliations

  1. Programa de Pós Graduação em Medicina Veterinária, Departamento de Clínica de Pequenos Animais, Hospital Veterinário Universitário, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil

    Rovaina L. Doyle, Camila B. Oliveira, Raqueli T. França, Sonia T. A. Lopes & Cinthia M. Andrade

  2. Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Chapecó, Santa Catarina, Brazil

    Aleksandro S. da Silva

  3. Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Setor de Bioquímica e Estresse Oxidativo do Laboratório de Terapia Celular, UFSM, Santa Maria, Rio Grande do Sul, Brazil

    Fátima H. Abdalla, Fabiano B. Carvalho & Cinthia M. Andrade

  4. Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Laboratório de Enzimologia Toxicológica, Departamento de Química, UFSM, Santa Maria, Rio Grande do Sul, Brazil

    Pauline Costa

  5. Instituto de Pesquisas Veterinárias Desidério Finamor, FEPAGRO Saúde Animal, Eldorado do Sul, Rio Grande do Sul, Brazil

    Rovaina L. Doyle, Guilherme M. Klafke & João R. Martins

Authors
  1. Rovaina L. Doyle
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  2. Aleksandro S. da Silva
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  3. Camila B. Oliveira
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  4. Raqueli T. França
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  5. Fátima H. Abdalla
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  6. Pauline Costa
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  7. Fabiano B. Carvalho
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  8. Guilherme M. Klafke
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  9. João R. Martins
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  10. Sonia T. A. Lopes
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  11. Cinthia M. Andrade
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Corresponding authors

Correspondence to Rovaina L. Doyle or Cinthia M. Andrade.

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Doyle, R.L., da Silva, A.S., Oliveira, C.B. et al. Lipid peroxidation and decrease on the activities of antioxidant enzymes in experimental infection by Babesia bovis in cattle. Comp Clin Pathol 24, 967–970 (2015). https://doi.org/10.1007/s00580-015-2077-0

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  • DOI: https://doi.org/10.1007/s00580-015-2077-0

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