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Comparative Clinical Pathology

, Volume 23, Issue 6, pp 1733–1742 | Cite as

Antidiabetic effect of Artemisia absinthium extracts on alloxan-induced diabetic rats

  • Haytham M. DaradkaEmail author
  • Mohaned Mohamed Abas
  • Mukhallad A. M. Mohammad
  • Mohamed Mousa Jaffar
Original Article

Abstract

The aim of our study was to evaluate the effect of Artemisia absinthium (A. absinthium) ethanol extract on alloxan-induced diabetic rats. Forty-eight albino rats (300 g) were used in this experiment and divided into six groups. Diabetes was induced in five rat groups by a single intraperitoneal injection of alloxan (150 mg/kg body weight). After hyperglycemia was confirmed, one group was considered as diabetic control and one group was treated with glibenclamide (10 mg/kg body weight/ daily) where the remaining three groups received daily treatments with three different doses of A. absinthium extract (250, 500, and 1,000 mg/kg body weight) for 10 days each dissolved in 0.2 ml distilled water was administered intraperitoneally to each corresponding rat group. Blood serum biochemical markers such as urea, creatinine, cholesterol, and total serum protein levels were recorded after the treatment ended. Findings indicate that treatment with medium and high doses of A. absinthium extract (500 and 1,000 mg/kg/body weight) reduces blood sugar values to significant levels (P < 0.01 and P < 0.001) in rats after 7 and 10 days of treatment when compared with diabetic control alloxan-induced rats in a similar fusion as in glibenclamide treatment (P < 0.001). All elevated blood serum markers induced by the alloxan treatment were reduced to significant levels in rats treated with A. absinthium at both medium and high doses (P < 0.01 and P < 0.001) and also after glibenclamide treatment (P < 0.001). We can conclude that A. absinthium treatment exhibits a significant antihyperglycemic effect without altering the body weight and can correct some biochemical markers induced by diabetes in a similar manner to glibenclamide treatment.

Keywords

Alloxan Diabetes A. absinthium Blood glucose Antihyperglycemic effect Biochemical markers 

References

  1. AlarconA FJ, Roman RR, Flores S JL, Aguirre GF (2002) Investigation on the hypoglycaemic effects of extracts of four Mexican medicinal plants in normal and alloxan-diabetic mice. Phytother Res 16:383–386CrossRefGoogle Scholar
  2. Alberti KG, Zimmet PZ (1998) Definition, diagnosis and classification of diabetes mellitus and its complications. Diabet Med 15:539–553PubMedCrossRefGoogle Scholar
  3. Alkhateeb H, Bonen A (2010) Thujone, a component of medicinal herbs, rescues palmitate-induced insulin resistance in skeletal muscle. Am J Physiol Regul Integr Comp Physiol 299:804–812CrossRefGoogle Scholar
  4. Al-Mustafa AH, Al-Thunibat OY (2008) Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes. Pak J Biol Sci 11:351–358PubMedCrossRefGoogle Scholar
  5. Anon (1937) The Papyrus Ebers: the greatest Egyptian medical document (E. Ebell,Trans.) Levin & Munksgaard, CopenhagenGoogle Scholar
  6. Baker P (2001) The book of absinthe: a cultural history, vol 2. Grove Press, New York, pp 99–100Google Scholar
  7. Betteridge DJ (1994) Diabetic dyslipidemia. Am J Med 96:25S–31SPubMedCrossRefGoogle Scholar
  8. Braunlich H, Marx F, Fleck C, Stein G (1997) Kidney function in rats after 5/6 nephrectomy (5/6 NX); effort of treatment with vitamin E. J Exp Toxicol Pathol 49:135–139CrossRefGoogle Scholar
  9. Canadanovic BJ, Djilas SM, Cetkovic GS, Tumbas VT (2004) Free-radical scavenging activity of wormwood Artemisia absinthium extracts. J Sci Food Agric 85:265–272CrossRefGoogle Scholar
  10. Clarke EG, Clarke ML (1975) Vet. Toxicol, vol 7, 3rd edn. Bailliere Tindal, New York, p 276Google Scholar
  11. Coppack SW, Jenson MD, Miles JM (1994) In vivo regulation of lipolysis in human. J Lipid Res 35:177–193PubMedGoogle Scholar
  12. Cordoba J, O’Riordan K, Dupuis J, Borensztajin J, Blei AT (1999) Diurnal variation of serum alanine transaminase activity in chronic liver disease. Hepatology 28:1724–1725CrossRefGoogle Scholar
  13. Denne SC, Liechty EA, Liu YM, Brechtel G, Baron AD (1991) Proteolysis in skeletal muscle and whole body in response to euglycemic hyperinsulinemia in normal adults. Am J Physiol 261:809–814Google Scholar
  14. Duby JJ, Campbell RK, Setter SM, White JR, Rasmussen KA (2004) Diabetic neuropathy: an intensive review. Am J Health Syst Pharm 61:160–173PubMedGoogle Scholar
  15. Dunn JS, Sheehan HL, Moletchie NG (1943) Necrosis of islets of Langerhans. Lancet 241:484–487CrossRefGoogle Scholar
  16. Ebbel B (1937) The Papyrus Ebers. Oxford University Press, Capenhagen, p 115Google Scholar
  17. Eddouks M, Maghrani M, Lemhadri A, Quahidi ML, Jouad H (2007) Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco. J Ethnopharmacol 82:97–103CrossRefGoogle Scholar
  18. Edwin E, Sheeja E, Gupta VB, Jain DC (2006) Fight diabetes the herbal way. Express Pharm Rev 1:41–42Google Scholar
  19. Eman GE, Helal AM et al (2005) Effect of Boswellia Carterii Birdw on carbohydrate metabolism in diabetic male Albino rats. Egypt J Hosp Med 20:38–45Google Scholar
  20. Gambelunghe C, Melai P (2007) Absinthe: enjoying a new popularity among young people? (Electronic version). Forensic Sci Int 130:183–186CrossRefGoogle Scholar
  21. Gilani AH, Janbaz KH (1995) Preventive and curative effects of Artemisia absinthium on acetaminophen and ccl4-induced hepatotoxicity. Gen Pharmacol 26:309–315PubMedCrossRefGoogle Scholar
  22. Gribel NV, Pashinskii VG (1991) New data on the antitumour activity of Artemisia absinthium L. Extract Rastitel’nye-Resursy 27:65–69Google Scholar
  23. Grieve MA (1980) Modern herbal. Penguin Books, London, pp 856–861Google Scholar
  24. Guarrera PM (2005) Traditional phytotherapy in Central Italy. J Ethnopharmacol 76:1–25Google Scholar
  25. Hruskova L, Danes V, Kliment V (1961) Determination of inhalation LD50 for guinea pigs and mice. Acta Virol 13:203–208Google Scholar
  26. Hwang DF, Lai YS, Chiang MT (1997) Toxic effects of grass carp, snake and chicken bile juices in rats. Toxicol Lett 85:85–92CrossRefGoogle Scholar
  27. Ikram M, Khattak SG, Gilani SN (1985) Antipyretic studies on some indigenous Pakistani medicinal plants. J Ethnopharmacol 14:45–51PubMedCrossRefGoogle Scholar
  28. Jasna MC, Sonja MD, Gordana SC, Vesna TT (2004) Free-radical scavenging activity of wormwood (Artemisia absinthium) extracts. J Sci Food Agric 85:265–272Google Scholar
  29. John S, Wilfred A, Timothy P (1999) Absinthe: what’s your poison? Though absinthe is intriguing, it is alcohol in general we should worry about. Br Med J 319:1590–1592CrossRefGoogle Scholar
  30. Kordali S, Cakir A, Mavi A, Kilic H, Yildirim A (2005) Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three Turkish Artemisia species. J Agric Food Chem 53:1408–1416PubMedCrossRefGoogle Scholar
  31. Lenzen S (2008) The mechanisms of alloxan and streptozotocin induced diabetes. Diabetologica 51:216–226CrossRefGoogle Scholar
  32. Lokesh D, Amit SD (2006) Diabetes mellitus—it’s possible pharmacological evaluation techniques and naturotherapy. Int J Green Pharm 1:15–28Google Scholar
  33. Lopes-Lutz D, Alviano DS, Alviano CS, Kolodziejczyk PP (2008) Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry 69:1732–1738PubMedCrossRefGoogle Scholar
  34. Lott JA, Wolf PL (1986) Alanine and aspartate aminotransferase (ALT and AST). In: Lott, JA, Wolf, PL (eds) Clinical enzymology. Yearbook Medical Publishers, Chicago, pp 111–157Google Scholar
  35. Lym RG, Messersmith CG, Dexter AG (1984) Absinth wormwood control. W-838. Fargo, ND: North Dakota State University, Cooperative Expernsion Service. 2 p. In cooperation with: U.S. Department of Agriculture. [23854]Google Scholar
  36. Maw MG, Thomas AG, Stahevitch A (1985) The biology of Canadian weeds Artemisia absinthium L. Can J Plant Sci 65:389–400CrossRefGoogle Scholar
  37. Muto T, Watanabe T, Okamura M, Moto M, Kashida Y, Mitsumori K (2003) Thirteen-week repeated dose toxicity study of wormwood (artemisia absinthium) extract in rats. J Toxicol Sci 28:471–478PubMedCrossRefGoogle Scholar
  38. Nathan MD (2008) The absinthe encyclopedia. Oxygéneé Press, Burgess HillGoogle Scholar
  39. Nezhadali A, Parsa M (2010) Study of the volatile compounds in Artemisia absinthium from Iran using HS/SPME/GC/MS. Adv Appl Sci Res 1(3):174–179Google Scholar
  40. Nishikawa T, Edelstein D, Du XL et al (2000) Normalizing mitoch-ondrial superoxide production blocks three pathways of hyperglycemic damage. Nature 404:787–790PubMedCrossRefGoogle Scholar
  41. Ohno T, Horio F, Tanaka S, Terada M, Namikawa T, Kitoh J (2000) Fatty liver and hyperlipidemia in IDDM of streptozotocin treated shrews. Life Sci 66:125–131PubMedCrossRefGoogle Scholar
  42. Olsen RW (2000) Receptors. Absinthe and gamma-amino butyric acid type A receptor modulation and metabolic detoxification. Proc Natl Acad Sci U S A 97:4417–4418PubMedCentralPubMedCrossRefGoogle Scholar
  43. Orchard TJ (1990) Dyslipoproteinemia and diabetes. Endocrinol Metab Clin N Am 19:361–379Google Scholar
  44. Padosch SA, Lachenmeier DW, Kröner LU (2006) Absinthium: a fictitious 19th century syndrome with present impact. Subst Abuse Treat Prev Policy 1:14PubMedCentralPubMedCrossRefGoogle Scholar
  45. Palevitch D, Yaniv Z (1991) Medicinal plants of the holy land, vol 1. Tamuz-Modan, Tel Aviv, pp 24–29Google Scholar
  46. Peavy DE, Taylor JM, Jefferson LS (1985) Time course of changes in albumen synthesis and mRNA in diabetic rats. Am J Physiol 248:656–663Google Scholar
  47. Ramachandran A, Snehalatha C, Vijay V (2002) Burden of type 2 diabetes and its complications—the Indian scenario. Curr Sci 83:1471–1476Google Scholar
  48. Rucker G, Walter RD, Manns D, Mayer R (1991) Antimalarial activity of somenatural peroxides. Planta Med 57:295–296PubMedCrossRefGoogle Scholar
  49. Sanders LJ (2002) From Thebes to Toronto and the 21st century: an incredible journey. Diabetes Spectr 15:56–60CrossRefGoogle Scholar
  50. Shanmugasundaram KR, Panneerselvam SP, Shanmugasundaram ER (1983) Enzyme changes and glucose utilization in diabetic rabbit: the effect of Gymnema sylvestrae. R Br J Ethnopharmacol 7:205–216CrossRefGoogle Scholar
  51. Shanmugasundram ER, Gopinath KL, Shanmugasundram KR, Rajendran VM (1990) Possible regeneration of islets of langerhans in streptozotocin diabetic rats given Gymnema sylvestre leaf extract. J Ethnopharmacol 30:265–279CrossRefGoogle Scholar
  52. Sharma SB, Nasir A, Prabhu KM, Murthy PS, Gev G (2003) Hypoglycemic and hypolipidimic effect of ethanolic extract of seeds of Eugenia jambolana in alloxan-induced diabetic rabbits. J Ethnopharmacol 85:201–206PubMedCrossRefGoogle Scholar
  53. Sharma VK, Kuma S, Patel HJ, Hugar S (2010) Hypoglycemic activity of ficus glomerata in alloxan induced diabetic rats. Int J Pharm Sci Rev Res 1:18–22Google Scholar
  54. Sicree R, Shaw J, Zimmet P (2006) Diabetes and impaired glucose tolerance. In: Gan D (ed) Diabetes Atlas. International Diabetes Federation, vol 3, 3rd edn. International Diabetes Federation, Belgium, pp 15–103Google Scholar
  55. Simon JE, Chadwick AF, Craker LE (1984) Herbs: an indexed bibliography, 1971-1980. The scientific literature on selected herbs and medicinal plants of the temperate zone. Archon Books, Connecticut, p 770Google Scholar
  56. Siperstein MD, Raskin P, Burns H (1973) Electron microscopic quantification of diabetic microangiopathy. J Diabetes 22:514–527Google Scholar
  57. Torben H (2002) Genetics of Type 2 diabetes. Curr Sci 83:1477–1482Google Scholar
  58. Travlos GS, Morris RW, Elwell MR, Duke A, Rosenblum S, Thompson MB (1996) Frequency and relationship of clinical chemistry and liver and kidney histopathology findings in 13 week toxicity studies in rats. J Toxicol 107:17–29CrossRefGoogle Scholar
  59. Urger RH, Foster DW (1998) Diabetes mellitus. Williams’s text book of endocrinology. WB Saunders, Philadelphia, pp 973–1060, 1981Google Scholar
  60. Williamson EM (2003) Potter’s herbal encyclopaedia, 1st edn. Essex, England, p 528Google Scholar
  61. Willis T (1684) Pharmaceutice rationalis: or, an excercitation of the operations of medicines in humane bodies, in his practice of physick. London, sect. 3: chapt. 3Google Scholar

Copyright information

© Springer-Verlag London 2014

Authors and Affiliations

  • Haytham M. Daradka
    • 1
    • 3
    Email author
  • Mohaned Mohamed Abas
    • 2
  • Mukhallad A. M. Mohammad
    • 2
  • Mohamed Mousa Jaffar
    • 2
  1. 1.Department of Biological Sciences, Faculty of ScienceTaibah UniversityAl-Madinah Al-MunawwarahSaudi Arabia
  2. 2.Department of Physiology, Faculty of MedicineJordan University of Science & TechnologyIrbidJordan
  3. 3.Biology Department, Faculty of ScienceTaibah UniversityAl-Madinah Al-MunawwarahSaudi Arabia

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