Comparative Clinical Pathology

, Volume 23, Issue 5, pp 1321–1330 | Cite as

Detection of human cytomegalovirus in glioma tumor tissues

  • Narges Ahani
  • Abbas Nikravesh
  • Reza Shirkoohi
  • Mohsen Karimi Arzenani
  • Mohammad Rokouei
  • Madjid Alipour Eskandani
Original Article


A brain tumor that develops from glial cells is called a glioma. About half of all primary brain tumors form from glial cells. Gliomas are divided into subgroups, depending on the origin of the glial cells. Detection of human cytomegalovirus in tumor tissues of glioma patient was performed for the first time in Iran. These data show an association between human cytomegalovirus (HCMV) and malignant gliomas and suggest that HCMV may play an active role in glioma pathogenesis. Cytomegalovirus surgical biopsy specimens of glioma tissues (n = 28) and non-tumor brain tissues (n = 8) were obtained. Total DNA of specimens was extracted, and then, extracted DNA was evaluated by polymerase chain reaction for evidence of HCMV nucleic acids. Here, we show that a high percentage of malignant gliomas are infected by HCMV. Seventy-five percent of grade 4 glioma, 57 % of grade 3 glioma, and 33 % of grade 2 glioma were HCMV positive. All specimens of grade 1 glioma and control tissues were HCMV negative. The relationship between the grade of glioma and the presence of HCMV is significant (P value = 0.035). Fisher exact test was used for statistical analysis.


Detection Human cytomegaloviruses Glioma PCR 


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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Narges Ahani
    • 1
    • 2
  • Abbas Nikravesh
    • 3
  • Reza Shirkoohi
    • 4
  • Mohsen Karimi Arzenani
    • 5
  • Mohammad Rokouei
    • 6
  • Madjid Alipour Eskandani
    • 7
  1. 1.Faculty of ScienceUniversity of ZabolZabolIran
  2. 2.TehranIran
  3. 3.Department of Molecular Sciences, Faculty of MedicineNorth Khorasan University of Medical SciencesBojnurdIran
  4. 4.Genetic Group, Cancer Research Center, Cancer Institute of IranTehran University of Medical SciencesTehranIran
  5. 5.Department of Molecular MedicinePasteur Institute of IranTehranIran
  6. 6.Department of Animal Science, Faculty of AgricultureUniversity of ZabolZabolIran
  7. 7.Department of Food Hygiene, Faculty of Veterinary MedicineUniversity of ZabolZabolIran

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