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Comparative Clinical Pathology

, Volume 23, Issue 3, pp 717–724 | Cite as

Effects of dietary alternatives (probiotic, green tea powder, and Kombucha tea) as antimicrobial growth promoters on growth, ileal nutrient digestibility, blood parameters, and immune response of broiler chickens

  • M. Afsharmanesh
  • B. Sadaghi
Original Article

Abstract

A feeding trial was conducted to study the effects of alternative antimicrobial growth promoters (probiotic, Kombucha tea, and green tea powder) in wet, wheat-based diets on broiler performance, carcass yield, organ weights, immune response, and histomorphological measurements of the small intestine. One hundred and sixty-one-day-old male broiler chicks were randomly assigned to 1 of 4 dietary treatments for 6 weeks. The dietary treatments were: (a) control (1.2 g water/gram of dry feed), (b) control diet supplemented with 1 g/kg probiotic (8 × 105 colony forming units (CFU) of Bacillus subtilis/gram), (c) control diet plus 1.2 g/kg diet Kombucha tea (20 % concentration), and (d) control diet plus 10 g/kg green tea powder. The body weight and feed intake were significantly (P < 0.05) increased by the dietary inclusion of the Kombucha tea compared to the control and green tea-fed broilers. However, an improvement in performance traits was observed in broilers fed with the probiotics. Plasma lipids of birds fed the green tea powder diet consisted of lower cholesterol and TG and higher HDL than those of control birds. Protein digestibility was significantly higher (P < 0.05) for birds given the Kombucha tea supplement compared to that of the control birds. The probiotic diet increased (P < 0.05) the villus height/crypt depth ratio and the villus height in the duodenum. Overall, the results in this work show that Kombucha tea and probiotic supplements in feed display growth promoting effects similar to those of traditional antibiotics; therefore, these products might be alternatives to antibiotic growth promoters.

Keywords

Probiotic Kombucha tea Green tea powder Digestibility Morphometric Broiler 

Abbreviations

ANOVA

analysis of variance

CFU

colony forming units

EGCG

epigallocatechin gallate

HDL

high-density lipoprotein

SRBC

sheep red blood cell

BW

body weight

TG

triglycerides

PBS

phosphate buffer saline

DM

dry matter

AME

apparent metabolizable energy

GIT

gastro-intestinal tract

FCR

feed conversion ratio

Notes

Acknowledgments

The authors gratefully acknowledge Shahid Bahonar University of Kerman for financial support. The authors thank Dr. Jasmine Kharazmi for her constructive and helpful comments on this manuscript. We also thank to Erin Gleeson for improving the grammar and flow of the manuscript.

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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  1. 1.Department of Animal and Poultry ScienceShahid Bahonar University of KermanKermanIran

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