Advertisement

Comparative Clinical Pathology

, Volume 23, Issue 1, pp 15–20 | Cite as

Anti-leishmanial activities of selenium nanoparticles and selenium dioxide on Leishmania infantum

  • Saied Soflaei
  • Abdolhossein DalimiEmail author
  • Amir Abdoli
  • Mahdi Kamali
  • Vahid Nasiri
  • Mojtaba Shakibaie
  • Mahdi Tat
Original Article

Abstract

Leishmania infantum is one of the important causes of visceral leishmaniasis in many countries. There are different complications for treatment of leishmaniasis such as toxicity and drug resistant. So far, there isn’t any information about the effects of selenium nanoparticles and selenium dioxide (chemical form of selenium) on Leishmania parasites; hence, the aim of the present study is to investigate in vitro effects of six dilutions of these drugs on L. infantum. Anti-leishmanial activities were studied by adding different dilutions of 2.5, 5, 10, 25, 50, and 100 μg/ml of the drugs into promastigote cultures. Promastigote cytotoxicity was tested using the colorimetric MTT assay. Anti-amastigote activity was assessed in peritoneal macrophages of BALB/c mice. Also, cytotoxic effect of these drugs was evaluated on uninfected macrophages. The results showed that both of drugs have dose-dependent anti-leishmanial activities. Selenium NPs have more growth-inhibitory effect on promastigotes than SeO2; while the IC50 (50 % inhibitory concentration) was determined to be 25 and 50 μg/ml, respectively. The mean numbers of amastigotes per macrophage in selenium NPs-treated groups were less than SeO2-treated and control groups. The IC50 of selenium NPs was 10 μg/ml and SeO2 was 25 μg/ml for amastigotes. Also, the IC50 of selenium NPs and SeO2 for uninfected macrophages were calculated to be 100 and 50 μg/ml, respectively. In addition, selenium NPs has less cytotoxic effect than SeO2 on uninfected macrophages. These findings suggest that selenium NPs have more anti-leishmanial properties and less cytotoxic effects than SeO2 against L. infantum.

Keywords

Leishmaniasis Leishmania infantum Selenium nanoparticles Selenium dioxide Anti-leishmanial activities 

Abbreviations

Selenium NPs

Selenium nanoparticles

SeO2

Selenium dioxide

References

  1. Aït-Oudhia K, Gazanion E, Vergnes B, Oury B, Sereno D (2011) Leishmania antimony resistance: what we know what we can learn from the field. Parasitol Res 109(5):1225–1232PubMedCrossRefGoogle Scholar
  2. Allahverdiyev AM, Abamor ES, Bagirova M, Ustundag CB, Kaya C, Kaya F, Rafailovich M (2011) Antileishmanial effect of silver nanoparticles and their enhanced antiparasitic activity under ultraviolet light. Int J Nanomedicine 6:2705–2714PubMedCentralPubMedCrossRefGoogle Scholar
  3. Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M (2007) Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol 5(11):873–882PubMedCrossRefGoogle Scholar
  4. Chen WX, Cao XZ, Zhu RZ (2003) Effect of selenium dioxide on proliferation, apoptosis, and elomerase activity of human lung cancer cell line in vitro (in Chinese with English abstract). Chinese J Cancer 22(9):927–931Google Scholar
  5. Croft SL, Sundar S, Fairlamb AH (2006) Drug resistance in leishmaniasis. Clin Microbiol Rev 19(1):111–126PubMedCentralPubMedCrossRefGoogle Scholar
  6. Durand R, Paul M, Rivollet D, Houin R, Astier A, Deniau M (1997a) Activity of pentamidine-loaded methacrylate nanoparticles against Leishmania infantum in a mouse model. Int J Parasitol 27(11):1361–1367PubMedCrossRefGoogle Scholar
  7. Durand R, Paul M, Rivollet D, Fessi H, Houin R, Astier A, Deniau M (1997b) Activity of pentamidine-loaded poly (d,l-lactide) nanoparticles against Leishmania infantum in a murine model. Parasite 4(4):331–336PubMedGoogle Scholar
  8. Fusai T, Deniau M, Durand R, Bories C, Paul M, Rivollet D, Astier A, Houin R (1994) Action of pentamidine-bound nanoparticles against Leishmania on an in vivo model. Parasite 1(4):319–324PubMedGoogle Scholar
  9. GuanYi H, Ying Z, Qiang Z, Bin Z, LongPing (2010) Vacuolization and apoptosis induced by nano-selenium in HeLa cell line. Sci China Chem 53(11):2272–2278CrossRefGoogle Scholar
  10. Guerin PJ, Olliaro P, Sundar S, Boelaert M, Croft SL, Desjeux P, Wasunna MK, Bryceson AD (2002) Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda. Lancet Infect Dis 2(8):494–501PubMedCrossRefGoogle Scholar
  11. Huang B, Zhang J, Hou J, Chen C (2003) Free radical scavenging efficiency of Nano-Se in vitro. Free Radic Biol Med 35(7):805–813PubMedCrossRefGoogle Scholar
  12. Irache JM, Esparza I, Gamazo C, Agüeros M, Espuelas S (2011) Nanomedicine: novel approaches in human and veterinary therapeutics. Vet Parasitol 180(1–2):47–71PubMedCrossRefGoogle Scholar
  13. Kim BY, Rutka JT, Chan WC (2010) Nanomedicine. N Engl J Med 363(25):2434–2443PubMedCrossRefGoogle Scholar
  14. Kuppusamy UR, Wan YP, Chai JW, Kanthimathi MS, Kanthimathi MS, Kuppusamy UR (2005) A comparison between selenium dioxide and selenium methionine induced cytotoxicity in estrogen receptor negative and positive breast cancer cell lines. J Food Technol 3:269–273Google Scholar
  15. Maltezou HC (2010) Drug resistance in visceral leishmaniasis. J Biomed Biotechnol 1–8. doi: 10.1155/2010/617521
  16. Mohebali M, Rezayat MM, Gilani K, Sarkar S, Akhoundi B, Esmaeili J, Satvat T, Elikaee S, Charehdar S, Hooshyar H (2009) Nanosilver in the treatment of localized cutaneous leishmaniasis caused by Leishmania major (MRHO/IR/75/ER): an in vitro and in vivo study. DARU 17(4):285–289Google Scholar
  17. Murray HW, Berman JD, Davies CR, Saravia NG (2005) Advances in leishmaniasis. Lancet 366:1561–1577PubMedCrossRefGoogle Scholar
  18. Paris C, Loiseau PM, Bories C, Bréard J (2004) Miltefosine induces apoptosis-like death in Leishmania donovani promastigotes. Antimicrob Agents Chemother 48(3):852–859PubMedCentralPubMedCrossRefGoogle Scholar
  19. Rayman MP (2012) Selenium and human health. Lancet 379(9822):1256–1268Google Scholar
  20. Shakibaie M, Khorramizadeh MR, Faramarzi MA, Sabzevari O, Shahverdi AR (2010) Biosynthesis and recovery of selenium nanoparticles and the effects on matrix metalloproteinase-2 expression. Biotechnol Appl Biochem 56(1):7–15PubMedCrossRefGoogle Scholar
  21. Torabi N, Mohebali M, Shahverdi AR, Rezayat SM, Edrissian GH, Esmaeili J, Charehdar S (2011) Nanogold for the treatment of zoonotic cutaneous leishmaniasis caused by Leishmania major (MRHO/IR/75/ER): an animal trial with methanol extract of Eucalyptus camaldulensis. JPHS 1:13–16Google Scholar
  22. Torres-Santos EC, Rodrigues JM, Moreira DL, Kaplan MA, Rossi-Bergmann B (1999) Improvement of in vitro and in vivo antileishmanial activities of 2', 6'-dihydroxy-4'-methoxychalcone by entrapment in poly (d, l-lactide) nanoparticles. Antimicrob Agents Chemother 43(7):1776–1778PubMedCentralPubMedGoogle Scholar
  23. Tran PA, Webster TJ (2011) Selenium nanoparticles inhibit Staphylococcus aureus growth. Int J Nanomedicine 6:1553–1558PubMedCentralPubMedGoogle Scholar
  24. Verma NK, Dey CS (2004) Possible mechanism of miltefosine-mediated death of Leishmania donovani. Antimicrob Agents Chemother 48(8):3010–3015PubMedCentralPubMedCrossRefGoogle Scholar
  25. Wang H, Wei W, Zhang SY, Shen YX, Yue L, Wang NP, Xu SY (2005) Melatonin-selenium nanoparticles inhibit oxidative stress and protect against hepatic injury induced by Bacillus Calmette-Guérin/lipopolysaccharide in mice. J Pineal Res 39(2):156–163PubMedCrossRefGoogle Scholar
  26. Wang H, Zhang J, Yu H (2007) Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. Free Radic Biol Med 42(10):1524–1533PubMedCrossRefGoogle Scholar
  27. Yousefi R, Ghaffarifar F, Dalimi Asl A (2009) The effect of Alkanna tincturia and Peganum harmala extracts on Leishmania major (MRHO/IR/75/ER) in vitro. Iranian J Parasitol 4(1):40–47Google Scholar
  28. Zampa MF, Araújo IM, Costa V, Nery Costa CH, Santos JR Jr, Zucolotto V, Eiras C, Leite JR (2009) Leishmanicidal activity and immobilization of dermaseptin 01 antimicrobial peptides in ultrathin films for nanomedicine applications. Nanomedicine 5(3):352–358PubMedGoogle Scholar
  29. Zhang J, Wang H, Bao Y, Zhang L (2004) Nano red elemental selenium has no size effect in the induction of seleno-enzymes in both cultured cells and mice. Life Sci 75(2):237–244PubMedCrossRefGoogle Scholar
  30. Zhang J, Wang H, Yan X, Zhang L (2005) Comparison of short-term toxicity between Nano-Se and selenite in mice. Life Sci 76(10):1099–1109PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2012

Authors and Affiliations

  • Saied Soflaei
    • 1
  • Abdolhossein Dalimi
    • 1
    Email author
  • Amir Abdoli
    • 1
  • Mahdi Kamali
    • 2
  • Vahid Nasiri
    • 1
  • Mojtaba Shakibaie
    • 3
  • Mahdi Tat
    • 2
  1. 1.Department of Parasitology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  2. 2.Nano-Biotechnology Research CenterBaqiyatallah University of Medical SciencesTehranIran
  3. 3.Department of Pharmacognosy & Biotechnology, Faculty of PharmacyKerman University of Medical SciencesKermanIran

Personalised recommendations