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Comparative Clinical Pathology

, Volume 22, Issue 5, pp 815–821 | Cite as

Platelet measurements in rat, dog and mouse blood samples using the Sysmex XT-2000iV

  • R. A. Mathers
  • G. O. Evans
  • J. Bleby
Original Article

Abstract

In this study, blood platelet measurements were evaluated for three laboratory species—rat, dog and mouse—using the Sysmex XT-2000iV haematology analyser, which offers two alternative methods for counting platelets—electrical impedance and a combination of light scattering and fluorescence. The analytical performance was satisfactory in terms of imprecision, carry-over between samples and linearity. The rat platelet counts were stable for at least 24 h at 4 °C, but not at room temperature: There were marked reductions at 24 and 48 h for dogs at both 4 °C and room temperature. Mean platelet volumes increased with time for both rats and dogs. Using the XT-2000iV, results were obtained for platelet counts (×109/L) together with the mean platelet volume (femtolitres), platelet distribution width (femtolitres), platelet large cell ratio (percent) and plateletcrit (percent), and these were compared with results obtained with the Siemens Advia 120™ with its multispecies software. Differences were observed for platelet counts and other platelet measurements made with the two analysers and between counts made by light scattering vs. fluorimetry. Some of these differences are due to the technologies employed in gating the cell populations and expressions used to describe the platelet populations.

Keywords

Haematology analyser Sysmex XT-2000iV Advia 120 Rat blood Dog blood Mouse blood Animal platelets Platelet parameters 

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Copyright information

© Springer-Verlag London Limited 2012

Authors and Affiliations

  1. 1.AstraZeneca R+D CharnwoodLoughboroughUK
  2. 2.Sysmex UK LtdMilton KeynesUK

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