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Comparative Clinical Pathology

, Volume 21, Issue 6, pp 1187–1191 | Cite as

Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar estuary, India

  • P. Satheeshkumar
  • G. Ananthan
  • D. Senthil Kumar
  • L. Jagadeesan
Original Article

Abstract

Haematological parameters have been recognized as valuable tools for monitoring fish health. Haematological and serum biochemical parameters were studied and compared different feeding behaviour of teleost fishes. Three marine teleost fishes, Lates calcarifer (carnivores), Mugil cephalus (omnivores) and Chanos chanos (herbivores), were carried out in order to find out a normal range of blood parameters which would serve as baseline data for assessment of the health status of the fish as well as reference point for future comparative surveys. Blood parameters such as red blood cell count (RBC) and white blood cells count (WBC), haemoglobin, haematocrit, mean cell haemoglobin concentration (MCHC), mean cell volume (MCV), mean cell haemoglobin, glucose, protein, cholesterol and urea were estimated from teleost fishes of different trophic level. Statistical analysis revealed that differences in haematological parameters between marine fish were significant (P < 0.01). The result revealed that haematological RBC/WBC ratio, MCV and MCHC were significantly correlated at P < 0.05 level. The RBC/WBC level increased due to the decrease in WBC during the study. Blood serum biochemical parameters can be used for confirming the maturity and monitoring any changes in the quality of waters and related soils.

Keywords

Blood Fish Haematology Erythrocyte Serum biochemistry 

Notes

Acknowledgement

The authors would like to thank Dr. G. Manimehalai, HOD, Department of Zoology, Kandaswamy Kandar College, Paramathi-Velur, Tamil Nadu, India, for providing the lab facility.

References

  1. Annino JS (1976) Clinical chemistry principles and procedures, 4th edn. Little Brown and Company, BostonGoogle Scholar
  2. Bahmani M, Kazemi R, Donskaya P (2001) A comparative study of some hematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiol Biochem 24:135–140CrossRefGoogle Scholar
  3. Bano Y (1985) Sublethal stress of DDT on biochemical composition of catfish Clarias batrachus. Indian J Environ Health 27:230–236Google Scholar
  4. Blaxhall PC, Daisley KW (1973) Routine haematological methods for use with fish blood. J Fish Biol 5:771–781CrossRefGoogle Scholar
  5. Borges A, Scotti LV, Siqueira DR, Jurinitz DF, Wasswemann GF (2007) Biochemical composition of seminal plasma and annual variations in semen characteristics of jundia Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiol Biochem 31:45–53CrossRefGoogle Scholar
  6. Chen YE, Jin S, Wang GL (2005) Study on blood physiological and biochemical indices of Vibrio alginilyticus disease of Lateolabrax japonicas. J Ocean Tai Str 24:104–108Google Scholar
  7. Clark S, Whitemore DH, Mc Mahon RF (1976) Consideration of blood parameters of largemouth bass, Micropterus salmoides. J Fish Biol 14:147–154CrossRefGoogle Scholar
  8. Cnaani A, Tinman S, Avidar Y, Ron M, Hulata G (2004) Comparative study of biochemical parameters in response to stress in O. aureus, O. mossambicus and two strains of O. niloticus. Aquaculture Res 35:1434–1440CrossRefGoogle Scholar
  9. Coz-Rakovac R, Strunjak-perovic I, Hacmanjek M, Topic PN, Lipez Z, Sostaric B (2005) Blood chemistry and histological properties of wild and cultured sea bass (Dicentrarchus labrox) in the North Adriatic Sea. Vet Res Comm 29:677–687CrossRefGoogle Scholar
  10. Dacie JV, Lewis SM (1984) Practical haematology, 6th edn. ELBS and Churchill, LivingstonGoogle Scholar
  11. Das BC (1965) Age related trends in the blood chemistry and haematology of the Indian carp (Catla catla). Gerontologia 10:47–64CrossRefGoogle Scholar
  12. De Pedro N, Guijarro AE, Lopez-Patino MA, Marinez-Alvarez R, Delgado M (2005) Daily and seasonal variation in haematological and blood biochemical parameters in tench Tinca tinca. Aquaculture Res 36:85–96Google Scholar
  13. Engel DM, Davis EM (1964) Relationship between activity and blood composition in certain marine teleosts. Copeia 3:586–587CrossRefGoogle Scholar
  14. Fawcette JK, Scott JE (1960) Practical clinical biochemistry, 4th edn. Arnold Harold Varley. pp 119–122Google Scholar
  15. Folin O, Wu H (1920) Span diagnostic kits. J Biolog Chem 9:341–367Google Scholar
  16. Henry RJ (1968) Clincal chemistry, principles and techniques. Harper and Row, New York, pp 664–666Google Scholar
  17. Hill S (1982) A literature review of the blood chemistry of rainbow trout, Salmo gairdneri. J Fish Biol 20:535–569CrossRefGoogle Scholar
  18. Hrubec TC, Smith SA, Robertson JJ (2001) Age related in haematology and biochemistry of hybrid striped bass Chrysops morone axatilis. Vet Clin Pathol 30:8–15PubMedCrossRefGoogle Scholar
  19. Jawad LA, Al-Mukhtar MA, Ahmed HK (2004) The relationship between haematocrit and some biological parameters of the Indian shad, Tenualosa ilisha (Family Clupidae). Anim Biodivers Conserv 27:478–483Google Scholar
  20. Joseph John P (2007) Alteration of certain blood parameters of fresh water teleost Mystus vittatus after chronic exposure to Metasystox and Sevin. Fish Physiol Biochem 33:15–20CrossRefGoogle Scholar
  21. Kavadias S, Castritsi-Catharios J, Dessypris A (2004) Annual cycles of growth rate, feeding rate, food conversion, plasma glucose and plasma lipids in the population of European sea bass (Dicentrarchus labrax) farmed in floating marine cages. J Appl Ichthyol 19:29–34CrossRefGoogle Scholar
  22. Lermen CL, Lappe R, Crestani M, Vieira VP, Gioda CR, Schetinger MRC, Baldisserotto B, Moraes G, Morsch VM (2004) Effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen. Aquaculture 239:497–507CrossRefGoogle Scholar
  23. Molnar GY (1960) Methode der Blutentnahme fur haematologische untersuchungen bei Fishcen. J Zoo and Fish 9:101–106Google Scholar
  24. Ojolick EJ, Cusack R, Benfey TJ, Kerr SR (1995) Survival and growth of all female diploid and triploid Clarias macrocephalus. Fish Genetics Biotro Special Pub 52:79–86Google Scholar
  25. Rambhaskar B, Srinivasa Rao K (1986) Comparative haematology of ten species of marine fish from Visakhapatnam Coast. J Fish Biol 30:59–66CrossRefGoogle Scholar
  26. Skjervold PO, Fjaera SO, Ostby PB, Einen O (2001) Live-chilling and crowding stress before slaughter of Atlantic salmon (Salmo salar). Aquaculture 192:265–280CrossRefGoogle Scholar
  27. Stillwell EJ, Benfey TJ (1995) Haemoglobin level, metabolic rate and swimming performance of triploid brook trout Salvelinus fontinalis. Aquaculture 137:355–358CrossRefGoogle Scholar
  28. Strange JR (1980) Acclimation temperature influences cortisol and glucose concentrations in stressed channel catfish. Trans Am Fish Soc 109:298–303CrossRefGoogle Scholar
  29. Strickland JDH, Parsons TR (1972) A practical hand book of seawater analysis. Fish Res Board Can, pp 310Google Scholar
  30. Svobodova Z, Kroupova H, Modra H, Flajshans M, Randak T, Savina LV, Gela D (2008) Haematological profile of common carp spawners of various breeds. J App Ichthyol 24:55–59CrossRefGoogle Scholar
  31. Wintrobe MM (1974) Clinical haematology. Lea and Febiger, PhiladelphiaGoogle Scholar
  32. Xiaoyun Z, Mingyun L, Khalid A, Weinmin W (2009) Comparative of haematology and serum biochemistry of cultured and wild Dojo loach Misgurnus anguillicadatus. Fish Physiol Biochem 35:435–441CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2011

Authors and Affiliations

  • P. Satheeshkumar
    • 1
    • 2
  • G. Ananthan
    • 1
  • D. Senthil Kumar
    • 3
  • L. Jagadeesan
    • 1
  1. 1.Faculty of Marine Science, Centre of Advanced Study in Marine BiologyAnnamalai UniversityParangipettaiIndia
  2. 2.Central Marine Fisheries Research InstituteKochiIndia
  3. 3.Department of ZoologyKandaswamy Kandar Arts and Science CollegeParamathy-VelurIndia

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