Comparative Clinical Pathology

, Volume 21, Issue 5, pp 1065–1069 | Cite as

The prevalence of cutaneous leishmaniasis in school children in southwestern Iran, 2009

  • Hojat Asgari Nezhad
  • Mohammad Mirzaie
  • Iraj Sharifi
  • Mehdi Zarean
  • Mojtaba Norouzi
Original Article


This study was designed to assess the epidemiological characteristics and molecular characterization of the disease in school children in the city of Dehloran, southwestern Iran. A total of 7,555 school children from the city of Dehloran were surveyed as a descriptive cross-sectional study during autumn 2009. A questionnaire was completed, recording demographic and disease characteristics. Microscopic examination and kinetoplast DNA-PCR assay were carried out for leishmaniasis detection and species identification. The overall prevalence of scars and active lesions were 9.7% and 0.4%, respectively. The prevalence rate of cutaneous leishmaniasis (CL) was significantly different between males and females (P < 0.05). The highest rate of CL was detected in the age group of 10–14 years with significant differences (P < 0.05). Most of the lesions were on face (42%). Clinically, 76.5% of the patients had single lesion. Also the prevalence rate in non-native residents was significantly higher than natives (P < 0.05). PCR technique revealed all examined cases as Leishmania major. CL in Dehloran is mainly caused by L. major and measures should be planned for future control programs. In addition, further investigations are required to clarify the possible reservoirs and vectors in the area.


Cutaneous leishmaniasis Epidemiology Leishmania major Dehloran 



This paper is part of M.Sc. thesis. The authors would like to thank Mr. Valizadeh and the staff of the Veterinary Network of Dehloran county for their kind assistance.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Al-Samarai AM, AlObaidi HS (2009) Cutaneous leishmaniasis in Iraq. J Infect Dev Countries 3:123–129Google Scholar
  2. Alimoradi S, Hajjaran H, Mohebali M, Mansouri F (2009) Molecular identification of Leishmania species isolated from human cutaneous leishmaniasis by RAPD-PCR. Iran J Publ Health 38:44–50Google Scholar
  3. Ashford RW (2000) The leishmaniases as emerging and reemerging zoonoses. Int J Parasitol 30:1269–1281PubMedCrossRefGoogle Scholar
  4. Desjeux P (2004) Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27:305–318PubMedCrossRefGoogle Scholar
  5. Fazaeli A, Fouladi B, Hashemi-Shahri SM, Sharifi I (2008) Clinical features of cutaneous leishmaniasis and direct PCR based identification of parasite species in a new focus in south-east of Iran. Iran J Pub Health 37:44–51Google Scholar
  6. Fazaeli A, Fouladic B, Sharifi I (2009) Emergence of cutaneous leishmaniasis in a border area at south-east of Iran: an epidemiological survey. J Vector Borne Dis 46:36–42PubMedGoogle Scholar
  7. Hajjaran H, Mohebali M, Razavi MR, Rezaei S, Kazemi B, Edrissian GhH, Mojtabavi J, Hooshmand B (2004) Identification of Leishmania species isolated from human cutaneous leishmaniasis, using random amplified polymorphic DNA (RAPD-PCR). Iran J Publ Health 33:8–15Google Scholar
  8. Javadian E, Dehestani M, Nadim A, Rassi Y, Tahvildare-Bidruni GH, Seyedi-Rashti MA, Shadmehr A (1998) Confirmation of Tatera indica (Rodentia: Gerbillidae) as the main reservoir host of zoonotic cutaneous leishmaniasis in the west of Iran. Iran J Publ Health 27:55–60Google Scholar
  9. Mahboudi F, Abolhassani M, Tehrani SR, Azimi M, Asmar M (2002) Differentiation of old and new world leishmania species at complex and species levels by PCR. Scand J Infect Dis 34:756–758PubMedCrossRefGoogle Scholar
  10. Mahboudi F, Abolhassan M, Yaran M, Mobtaker H, Azizi M (2001) Identification and differentiation of Iranian Leishmania species by PCR amplification of kDNA. Scand J Infect Dis 33:596–598PubMedCrossRefGoogle Scholar
  11. Marfurt J, Niederwieser I, Makia ND, Beck HP, Felger I (2003) Genotyping of Old and New World Leishmania species by PCR-RFLP. Diagn Microbiol Infect Dis 46:115–124PubMedCrossRefGoogle Scholar
  12. Mengistu G, Akuffo H, Fehniger TE, Negese Y, Nilsen R (1992) Comparison of parasitological and immunological methods in the diagnosis of leishmaniasis in Ethiopia. Trans R Soc Trop Med Hyg 86:154–157PubMedCrossRefGoogle Scholar
  13. Momeni AZ, Aminjavaheri M (1994) Clinical picture of cutaneous leishmaniasis in Isfahan, Iran. Int J Dermatol 33:260–265PubMedCrossRefGoogle Scholar
  14. Rodgers MR, Popper SJ, Wirth DF (1990) Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol 71:267–275PubMedCrossRefGoogle Scholar
  15. Schonian G, Nasereddin A, Dinse N, Schweynoh C, Schalling HD, Presbo W, Jaffe C (2003) PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47:349–358PubMedCrossRefGoogle Scholar
  16. Sharifi I, Fekri AR, Aflatonian MR, Nadim A, Nikian Y, Kamesipour A (1998) Cutaneous leishmaniasis in primary school children in the south-eastern Iranian city of Bam, 1994–95. Bull WHO 76:289–293PubMedGoogle Scholar
  17. Smyth AJ, Ghosh A, Hassan MQ, Basu D, De Bruijn MH, Adhya S, Mallik KK, Barker DC (1992) Rapid and sensitive detection of Leishmania kinetoplast DNA from spleen and blood samples of kala-azar patients. Parasitol 105:183–192CrossRefGoogle Scholar
  18. Talari SA, Shajari G, Talaei R (2006a) Clinical finding of cutaneous leishmaniasis as a new focus of Iran. Internet J Infec Dis 5:1–5Google Scholar
  19. Talari SA, Talaei R, Shajari G, Vakili Z, Taghaviardakani A (2006b) Childhood cutaneous leishmaniasis: report of 117 cases from Iran. Korean J Parasitol 44:355–360PubMedCrossRefGoogle Scholar
  20. Tashakori M, Ajary S, Kariminia A, Mahboudi F, Alimohammadian MH (2003) Characterization of Leishmania species and L. major strains in different endemic areas of leishmaniasis in Iran. Iran Biomed J 7:45–50Google Scholar
  21. Yaghoobi-Ershadi MR, Akhavan AA (1999) Entomological survey of sand flies (Diptera: Psychodidae) in a new focus of zoonotic cutaneous leishmaniasis in Iran. Acta Trop 73:321–326PubMedCrossRefGoogle Scholar
  22. Yaghoobi-Ershadi MR, Hanafi-Bojd AA, Akhavan AA, Zahrai-Ramazani AR, Mohebali M (2001) Epidemiological study in a new focus of cutaneous leishmaniasis due to Leishmania major in Ardestan town, central Iran. Acta Trop 79:115–121PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2011

Authors and Affiliations

  • Hojat Asgari Nezhad
    • 1
    • 2
  • Mohammad Mirzaie
    • 3
  • Iraj Sharifi
    • 4
  • Mehdi Zarean
    • 4
  • Mojtaba Norouzi
    • 5
  1. 1.Student of Veterinary Parasitology, School of Veterinary MedicineShahid Bahonar University of KermanKermanIran
  2. 2.Young Researchers SocietyShahid Bahonar University of KermanKermanIran
  3. 3.Department of Pathobiology, School of Veterinary MedicineShahid Bahonar University of KermanKermanIran
  4. 4.Dermatology and Leishmaniasis Research CenterKerman University of Medical ScienceKermanIran
  5. 5.Razi Vaccine and Serum Research InstituteKarajIran

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