Effects of different dietary prebiotic inulin levels on blood serum enzymes, hematologic, and biochemical parameters of great sturgeon (Huso huso) juveniles
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An 8-week growth trial was carried out in freshwater fiberglass tanks to investigate the effects of different dietary prebiotic inulin levels on hematologic and biochemical parameters and some blood serum enzymes in juvenile great sturgeon (Huso huso). Four experimental diets were formulated to contain different prebiotic inulin levels (0.0%, 1.0%, 2.0%, and 3.0%, respectively). Triplicate groups of fish (50 fish per tank with an average body weight of 16.14 ± 0.38 g) were assigned to each diet. Blood samples were taken from the caudal vein of 60 apparently healthy fish at the end of the trial. No significant differences were observed in serum enzymes between the treatment groups (P > 0.05). The results show that with the increase in supplementation level of inulin, the mean values of alkaline phosphatase decreased but the mean value of other enzymes increased. white blood cell count increased significantly in group treated with 1% inulin compared with the other groups (P < 0.05). No significant difference between the treatment groups was observed in some hematologic and biochemical parameters such as red blood cell count, mean corpuscular hemoglobin (MCH), and glucose (P > 0.05). With the increase supplementation level of inulin, the mean values of cholesterol, MCH, and MCH concentration increased, but the mean value of glucose, white blood cell count, red blood cell count, hematocrit, hemoglobin, and mean corpuscular volume decreased.
KeywordsBiochemical parameters Enzyme Hematology Prebiotic inulin Great sturgeon
This work was carried out in Shaheed Marjani sturgeon fish breeding and rearing center (Gorgan, Iran). We express our tanks to this center expert.
- Affonso EG, Polez VLP, Correa CF, Mazoa AF, Araujo MRR, Moraes G (2002) Blood parameters and metabolites in teleost fish Colossoma macropomum exposed to sulfide or hypoxia. Comp Biochem Physiol C 33:375–382Google Scholar
- Ballarin L, Dalloro M, Bertotto D, Libertini A, Francescon A, Barbaro A (2004) Haematological parameters in Umbriana cirrosa (Teleostei, Scianidae): a comparison between diploid and triploid specimen. Comp Biochem Physiol A 183:45–51Google Scholar
- Findeis EK (1997) Osteology and phylogenetic relationships of recent sturgeons. In: Birstein VJ, Waldman JR, Bemis WE (eds) Sturgeon. biodiversity and conservation. Kluwer Academic Publishers, Dordrecht, Germany, pp 73–106Google Scholar
- Jalali MA, Ahmadifar E, Sudagar M, Azari Takami GH (2009) Growth efficiency, body composition, survival and haematological changes in great sturgeon (Huso huso Linnaeus, 1758) juveniles fed diets supplemented with different levels of Ergosan. Aquac Res. doi: 10.1111/j.1365-2109.2009.02166.x Google Scholar
- Klontz GW (1994) Fish hematology. In: Stolen JS, Fletcher TC, Rowley AF, Kelikoff TC, Kaattari SL, Smith SA (eds) Techniques in Fish Immunology, vol. 3. SOS Publications, Fair Haven, NJ, pp 121–132Google Scholar
- Rowley AF (1990) Collection, separation and identification of fish leukocytes. In: van Muiswinkel WB (ed) Techniques in fish immunology—I. SOS Publications, Fair Haven, NJ, pp 113–136Google Scholar
- Seiverd CE (1964) Hematology for medical technologists. Lea and Febiger, Philadelphia, PA, p 946Google Scholar
- Shalaby A (2005) The opposing effect of ascorbic acid (Vitamin C) on Ochratoxin toxicity in Nile tilapia, Oreochromis niloticus. 6th Intern. Symp. Tilapia in Aquac. Philipin, pp 150–157Google Scholar
- Snieszko SF (1960) Microhaematocrit as a tool in fisheries management. Special Scientific Report—Fisheries. No.314.U.S. Department of the Interior. Fish and Fisheries Wildlife Washington, DCGoogle Scholar
- Stoskopf MK (1993a) Fish medicine. WB Saunders Company, Philadelphia, PA, p 882Google Scholar
- Stoskopf MA (1993b) Fish medicine. WB Sounders, Philadelphia, PA, 882 ppGoogle Scholar