Comparative Clinical Pathology

, Volume 19, Issue 4, pp 437–439

Drug resistance of Salmonella spp. isolated from pigeon eggs

Brief Communication

Abstract

Avian Salmonellae infections cause not only clinical disease in poultry but are also recognized as a source of food-borne disease transmission to humans. Domestic pigeons usually live in urban and rural areas in close proximity to human residential areas. Although their role in transmission of different infectious organisms is important from public health point of view, there are few reports available on Salmonella infections and their resistance to antibiotics in pigeon. In order to study pigeon egg contamination to the Salmonella spp. and the drug resistance pattern of the isolated organisms, pigeon egg shells were cleaned and disinfected with 96% ethanol. The contents of each egg was emptied into a sterile petri dish and incubated at 37°C for 24 h. Isolates were finally confirmed by PCR, using specific primers targeting the invA gene sequences from Salmonella spp. The results of this study showed that 5.7% of pigeon eggs had Salmonella spp. contamination. Disk diffusion test on Muller–Hinton agar was used to determine the sensitivity to antibacterial agents. Study of resistance to antimicrobial agents of isolated Salmonella spp. among the ten antibiotics showed a high frequency of sensitivity to ciprofloxacin, norfloxacin, gentamycin, and chloramphenicol (100%), and a high level of resistance to tetracycline (50%) was observed.

Keywords

Pigeon eggs Antimicrobial resistance Salmonella spp. 

References

  1. Adams RH (1995) Veterinary pharmacology and therapeutics, 7th edn. Iowa State University Press, Iowa, pp 1112–1114Google Scholar
  2. Adesiym A, Offiah N, Seepersadsingh N, Rodrigo S, Lashley V, Musai L (2007) Antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from table eggs. Food Control 18:306–311CrossRefGoogle Scholar
  3. Byrd JA, DeLoach JR, Corrier DE, Nisbet DJ, Stanker LH (1999) Evaluation of Salmonella serotype distributions from commercial broiler hatcheries and grower houses. Avian Dis 43:39–47CrossRefPubMedGoogle Scholar
  4. Caldwell DJ, Hargis BM, Corrier DE, Vidal L, DeLoach JR (1995) Evaluation of persistence and distribution of Salmonella serotype isolation from poultry farms using drag-swabs sampling. Avian Dis 39:617–621CrossRefPubMedGoogle Scholar
  5. Cortez ALL, Carvalho ACFB, Ikuno AA, Burger KP, Vidal-Martins AMC (2006) Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR. Res Vet Sci 81:340–344CrossRefPubMedGoogle Scholar
  6. Fukata T, Uemura T, Baba E, Horiuchi S, Arakawa A (1986) Isolation of clostridia, salmonella and coccidian from wild pigeons in Japan. Br Vet J 142:291–293PubMedGoogle Scholar
  7. Glynn MK, Bopp C, Dewitt W, Dabney P, Mokhtar M, Angulo FJ (1998) Emergence of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 infections in the United States. N Engl J Med 338:1333–1338CrossRefPubMedGoogle Scholar
  8. Graziani C, Busani L, Dionisi AM, Lucarelli C, Owczarek S, Ricci A, Mancin M, Caprioli A, Luzzi I (2008) Antimicrobial resistance in Salmonella enterica serovar Typhimurium from human and animal sources in Italy. Vet Microbial 128:414–418CrossRefGoogle Scholar
  9. Nazer AHK, Safari GH (1994) Bacterial flora from dead-in-shell chicken embryos and their drug resistance in Fars Province of Iran. Ind J of Anim Sci 64(10):1006–1009Google Scholar
  10. Pan Z, Wang X, Zhang X, Geng S, Chen X, Pan W, Cong Q, Liu X, Jiao X, Liu X (2009) Changes in antimicrobial resistance among Salmonella enterica subspecies enterica serovar pullorum isolates in China from 1962 to 2007. Vet Microbiol 136:387–392CrossRefPubMedGoogle Scholar
  11. Quinn PJ, Carter ME, Markey B, Carter GR (1994) Clinical veterinary microbiology. Wolf Publishing, London, pp 95–102Google Scholar
  12. Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC (2002) Veterinary Microbiology and Microbial disease. Blackwell Science, Oxford, pp 113–118Google Scholar
  13. Rajesh C, Rao VDP, Gomez-Villamandos JC, Shukla SK, Banerjee PS (2001) Diseases of poultry and their control, 1st edn. International Book Distributing, Lucknow, pp 74–82Google Scholar
  14. Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR, Swayne DE (2003) Diseases of poultry, 11th edn. Iowa, Iowa State Press, pp 583–599Google Scholar
  15. Sawa H, Hirai K (1981) An outbreak of Salmonella typhimurium subserovar copenhagen infection in pigeons (Chalcophaps indica) imported from Hong Kong. J Jpn Vet Sci 43:277–279Google Scholar
  16. Siqueira RS, Dodd CER, Rees CED (2003) Phage amplification assay as rapid method for Salmonella detection. Braz J Microbiol 34:118–120CrossRefGoogle Scholar
  17. Swayne DE, Glisson JR, Jackwood MW, Pearson JE, Reed WM (1998) A laboratory manual for the isolation and identification of avian pathogens, 4th edn. American Association of Avian Pathologists, University of Pennsylvania, Kennett Square, PA, pp 4–13Google Scholar
  18. Van Duijkeren E, Wannet WJB, Houwers DJ, Van Pelt W (2003) Antimicrobial susceptibilities of Salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001. J Clin Microbiol 41:3574–3578CrossRefPubMedGoogle Scholar
  19. Woodward MJ, Kirwan SES (1996) Detection of Salmonella enteritidis in eggs by the polymerase chain reaction. Vet Rec 138:411–413PubMedGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2010

Authors and Affiliations

  1. 1.Department of Poultry Diseases, School of Veterinary MedicineUniversity of ZabolZabolIran
  2. 2.Avian Diseases Research Center, School of Veterinary MedicineUniversity of ShirazShirazIran

Personalised recommendations