Comparative Clinical Pathology

, Volume 19, Issue 3, pp 235–239 | Cite as

The dextran sulphate sodium (DSS) model of colitis: an overview

  • Livingstone Solomon
  • Sorsiah Mansor
  • Peter Mallon
  • Eilish Donnelly
  • Margaret Hoper
  • Morris Loughrey
  • Stephen Kirk
  • Keith Gardiner
Review Article

Abstract

The dextran sulphate sodium model of colitis has demonstrated several correlations with human inflammatory bowel disease and is deemed suitable for investigating pathogenesis, therapeutic options and the dysplasia–adenocarcinoma sequence of inflammatory bowel disease. It is widely applicable to mice, rats, hamsters and guinea pigs. This review explores the features of this model and identifies areas for further research studies.

Keywords

Dextran sulphate sodium Colitis Inflammatory bowel diseases 

References

  1. Axelsson LG, Landstrom E, Goldschmidt TJ, Gronberg A, Bylund-Fellenius AC (1996) Dextran sulfate sodium (dss) induced experimental colitis in immunodeficient mice: effects in cd4(+) -cell depleted, athymic and nk-cell depleted scid mice. Inflamm Res 45:181–191CrossRefPubMedGoogle Scholar
  2. Axelsson L, Landstrom E, Bylund-Fellenius A (1998) Experimental colitis induced by dextran sulphate sodium in mice: beneficial effects of sulphasalazine and olsalazine. Aliment Pharmacol Ther 12:925CrossRefPubMedGoogle Scholar
  3. Bailey RW, Bourne EJ (1961) Intracellular glycosidases of dextran-producing bacteria. Nature 191:277–278CrossRefPubMedGoogle Scholar
  4. Breider MA, Eppinger M, Gough A (1997) Intercellular adhesion molecule-1 expression in dextran sodium sulfate-induced colitis in rats. Vet Pathol 34:598–604CrossRefPubMedGoogle Scholar
  5. Chen Y, Chou K, Fuchs E, Havran WL, Boismenu R (2002) Protection of the intestinal mucosa by intraepithelial gamma delta t cells. Proc Natl Acad Sci U S A 99:14338–14343CrossRefPubMedGoogle Scholar
  6. Chiba T (1993) cell kinetics of carcinoma originating from rat colitis induced by dextran sulphate sodium. Nippon Shokakibyo Gakkai Zasshi 90:774–781PubMedGoogle Scholar
  7. Clapper ML, Cooper HS, Chang WC (2007) Dextran sulfate sodium-induced colitis-associated neoplasia: a promising model for the development of chemopreventive interventions. Acta Pharmacol Sin 28:1450–1459CrossRefPubMedGoogle Scholar
  8. Cooper HS, Murthy SN, Shah RS, Sedergran DJ (1993) Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest 69:238–249PubMedGoogle Scholar
  9. Cooper HS, Murthy S, Kido K, Yoshitake H, Flanigan A (2000) Dysplasia and cancer in the dextran sulfate sodium mouse colitis model. Relevance to colitis-associated neoplasia in the human: a study of histopathology, b-catenin and p53 expression and the role of inflammation. Carcinogenesis 21:757–768CrossRefPubMedGoogle Scholar
  10. Dieleman LA, Palmen MJ, Akol H, Bloemena E, Pena AS, Meuwissen SG, Van Rees EP (1998) Chronic experimental colitis induced by dextran sulphate sodium (dss) is characterized by th1 and th2 cytokines. Clin Exp Immunol 114:385–391CrossRefPubMedGoogle Scholar
  11. Egger B, Bajaj-Elliott M, MacDonald TT, Inglin R, Eysselein VE, Buchler MW (2000) Characterisation of acute murine dextran sodium sulphate colitis: cytokine profile and dose dependency. Digestion 62:240–248CrossRefPubMedGoogle Scholar
  12. Elson CO, Sartor RB, Tennyson GS, Riddell RH (1995) Experimental models of inflammatory bowel disease. Gastroenterology 109:1344–1367CrossRefPubMedGoogle Scholar
  13. Elson C, Cong Y, Brandwein S et al (1998) Experimental models to study molecular mechanisms underlying intestinal inflammation. Ann N Y Acad Sci 859:85–95CrossRefPubMedGoogle Scholar
  14. Gaudio E, Taddei G, Vetuschi A, Sferra R, Frieri G, Ricciardi G, Caprilli R (1999) Dextran sulfate sodium (dss) colitis in rats: clinical, structural, and ultrastructural aspects. Dig Dis Sci 44:1458–1475CrossRefPubMedGoogle Scholar
  15. Geier MS, Smith CL, Butler RN, Howarth GS (2008) Small-intestinal manifestations of dextran sulfate sodium consumption in rats and assessment of the effects of lactobacillus fermentum br11. Dig Dis Sci 54:1222–1228CrossRefPubMedGoogle Scholar
  16. Hans W, Scholmerich J, Gross V, Falk W (2000) The role of the resident intestinal flora in acute and chronic dextran sulfate sodium-induced colitis in mice. Eur J Gastroenterol Hepatol 12:267–273CrossRefPubMedGoogle Scholar
  17. Iwanaga T, Hoshi O, Han H, Fujita T (1994) Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: some possible mechanisms of cecal ulceration. J Gastroenterol 29:430–438CrossRefPubMedGoogle Scholar
  18. Kitajima S, Takuma S, Morimoto M (1999a) Changes in colonic mucosal permeability in mouse colitis induced with dextran sulfate sodium. Exp Anim 48:137–143CrossRefPubMedGoogle Scholar
  19. Kitajima S, Takuma S, Morimoto M (1999b) Tissue distribution of dextran sulfate sodium (dss) in the acute phase of murine dss-induced colitis. J Vet Med Sci 61:67–70CrossRefPubMedGoogle Scholar
  20. Kitajima S, Morimoto M, Sagara E, Shimizu C, Ikeda Y (2001) Dextran sodium sulfate-induced colitis in germ-free iqi/jic mice. Exp Anim 50:387–395CrossRefPubMedGoogle Scholar
  21. Kullmann F, Messmann H, Alt M, Gross V, Bocker T, Scholmerich J, Ruschoff J (2001) Clinical and histopathological features of dextran sulfate sodium induced acute and chronic colitis associated with dysplasia in rats. Int J Colorectal Dis 16:238–246CrossRefPubMedGoogle Scholar
  22. Mahler M, Bristol IJ, Leiter EH, Workman AE, Birkenmeier EH, Elson CO, Sundberg JP (1998) Differential susceptibility of inbred mouse strains to dextran sulfate sodium-induced colitis. Am J Physiol 274:G544–551PubMedGoogle Scholar
  23. Mahler M, Bristol IJ, Sundberg JP, Churchill GA, Birkenmeier EH, Elson CO, Leiter EH (1999) Genetic analysis of susceptibility to dextran sulfate sodium-induced colitis in mice. Genomics 55:147–156CrossRefPubMedGoogle Scholar
  24. Mallon PT, Mckenna M, Kirk SJ, Gardiner KR (2006) Dextran sulfate sodium (DSS) induced colitis reduces mucosal barrier function in Sprague-Dawley rats. Canadian Digestive Diseases Week (CDDW) February 24th–February 27th, 2006 ed. Banff, Alberta, Canadian Association of GastroenterologyGoogle Scholar
  25. Marcus R, Watt J (1969) Seaweeds and ulcerative colitis in laboratory animals. Lancet 2:489–490CrossRefPubMedGoogle Scholar
  26. Marcus R, Watt J (1971) Colonic ulceration in young rats fed degraded carrageenan. Lancet 2:765–766CrossRefPubMedGoogle Scholar
  27. Murthy SN, Cooper HS, Shim H, Shah RS, Ibrahim SA, Sedergran DJ (1993) Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin. Dig Dis Sci 38:1722–1734CrossRefPubMedGoogle Scholar
  28. Murthy S, Cooper HS, Yoshitake H, Meyer C, Meyer CJ, Murthy NS (1999) Combination therapy of pentoxifylline and tnfalpha monoclonal antibody in dextran sulphate-induced mouse colitis. Aliment Pharmacol Ther 13:251–260CrossRefPubMedGoogle Scholar
  29. Ni J, Chen SF, Hollander D (1996) Effects of dextran sulphate sodium on intestinal epithelial cells and intestinal lymphocytes. Gut 39:234–241CrossRefPubMedGoogle Scholar
  30. Ohkusa T (1985) production of experimental ulcerative colitis in hamsters by dextran sulfate sodium and changes in intestinal microflora. Nippon Shokakibyo Gakkai Zasshi 82:1327–1336PubMedGoogle Scholar
  31. Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R (1990) A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology 98:694–702PubMedGoogle Scholar
  32. Poritz LS, Garver KI, Green C, Fitzpatrick L, Ruggiero F, Koltun WA (2007) Loss of the tight junction protein zo-1 in dextran sulfate sodium induced colitis. J Surg Res 140:12–19CrossRefPubMedGoogle Scholar
  33. Stevceva L, Pavli P, Husband AJ, Doe WF (2001) The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of dss used to induce it. BMC Clin Pathol 1:3CrossRefPubMedGoogle Scholar
  34. van Meeteren ME, Meijssen MA, Zijlstra FJ (2000) The effect of dexamethasone treatment on murine colitis. Scand J Gastroenterol 35:517–521CrossRefPubMedGoogle Scholar
  35. Venkatraman A, Ramakrishna BS, Pulimood AB, Patra S, Murthy S (2000) Increased permeability in dextran sulphate colitis in rats: time course of development and effect of butyrate. Scand J Gastroenterol 35:1053–1059CrossRefPubMedGoogle Scholar
  36. Verdu EF, Bercik P, Cukrowska B, Farre-Castany MA, Bouzourene H, Saraga E, Blum AL, Corthesy-Theulaz I, Tlaskalova-Hogenova H, Michetti P (2000) Oral administration of antigens from intestinal flora anaerobic bacteria reduces the severity of experimental acute colitis in balb/c mice. Clin Exp Immunol 120:46–50CrossRefPubMedGoogle Scholar
  37. Watt J, Marcus R (1969) Ulcerative colitis in the guinea-pig caused by seaweed extract. J Pharm Pharmacol 21(Suppl):187S+Google Scholar
  38. Yamada M, Ohkusa T, Okayasu I (1992) Occurrence of dysplasia and adenocarcinoma after experimental chronic ulcerative colitis in hamsters induced by dextran sulphate sodium. Gut 33:1521–1527CrossRefPubMedGoogle Scholar
  39. Yoshida Y, Iwai A, Itoh K, Tanaka M, Kato S, Hokari R, Miyahara T, Koyama H, Miura S, Kobayashi M (2000) Role of inducible nitric oxide synthase in dextran sulphate sodium-induced colitis. Aliment Pharmacol Ther 14:26–32CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2010

Authors and Affiliations

  • Livingstone Solomon
    • 1
  • Sorsiah Mansor
    • 2
  • Peter Mallon
    • 1
  • Eilish Donnelly
    • 1
  • Margaret Hoper
    • 1
  • Morris Loughrey
    • 2
  • Stephen Kirk
    • 1
  • Keith Gardiner
    • 1
  1. 1.Institute of Clinical Sciences, Department of SurgeryQueens University of BelfastBelfastUK
  2. 2.Department of PathologyQueens University of BelfastBelfastUK

Personalised recommendations