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Comparative Clinical Pathology

, Volume 19, Issue 4, pp 377–381 | Cite as

In vitro effects of pentoxifylline on kinematic parameters, capacitation, and acrosome reaction of ram epididymal sperm

  • Hossein Hassanpour
  • Pezhman Mirshokraei
  • Parviz Tajik
  • Abbas Haghparast
Original Article

Abstract

To evaluate effects of different concentrations of pentoxifylline, as phosphodiesterase inhibitor, on kinematic parameters, capacitation, and acrosome reaction, spermatozoa were separated from ram caudal epididymis. Epididymal sperm were incubated at concentrations of 0.01 mM, 0.1 mM, 1 mM, and 10 mM pentoxifylline for 60 min. Motion parameters were assessed using the CASA system. Capacitation and acrosome reaction were also evaluated by CTC staining. Pentoxifylline at 0.01 mM appeared significant (P < 0.05) in increasing progressive motility, straight line velocity (VSL), VCL, average path velocity (VAP), average amplitude of lateral head displacement in micrometers (ALH), and mean angular displacement (MAD) and significant (P < 0.05) in decreasing BCF in spermatozoa compared with controls. Pentoxifylline at 10 mM caused a significant (P < 0.05) decrease in progressive motility, VSL, VAP, ALH, LIN, and WOB compared with controls. There was a significant (P < 0.05) reduction in uncapacitated spermatozoa but an insignificant rise in capacitated and acrosome-reacted spermatozoa in 0.01 mM pentoxifylline when compared with controls. Capacitated spermatozoa were significantly (P < 0.05) lower in 0.1 mM pentoxifylline, whereas capacitated and acrosome-reacted spermatozoa were insignificantly higher than controls. In 10 mM pentoxifylline, significant increase was observed in capacitated and significant decrease in acrosome-reacted spermatozoa. In conclusion, a low concentration of pentoxifylline was able to increase most kinematic parameters while high concentration had the opposite effect. Pentoxifylline in high concentration increased capacitation but decreased acrosome reaction.

Keywords

Pentoxifylline Ram Spermatozoa Kinematic parameters Acrosome status 

Notes

Acknowledgments

This work had been supported by the funds granted by the Applied Research Centre, Vice Chancellor for Research of Shahrekord University.

References

  1. Ain R, Uma Devi K, Shivaji S, Seshagiri PB (1999) Pentoxifylline-stimulated capacitation and acrosome reaction in hamster spermatozoa: involvement of intracellular signalling molecules. Mol Hum Reprod 5:618–626CrossRefPubMedGoogle Scholar
  2. Bag S, Joshi A, Rawat PS, Mittal JP (2002) Effect of initial freezing temperature on the semen characteristics of frozen–thawed ram spermatozoa in a semi-arid tropical environment. Small Rumin Res 43:23–29CrossRefGoogle Scholar
  3. Blash S, Melican D, Gavin W (2000) Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology 54:899–905CrossRefPubMedGoogle Scholar
  4. Brucker C, Lipford GB (1995) The human sperm acrosome reaction: physiology and regulatory mechanisms. An update. Hum Reprod Updat 1:51–62CrossRefGoogle Scholar
  5. Calogero AE (1998) Correlation between intracellular cAMP content, kinematic parameters and hyperactivation of human spermatozoa after incubation with pentoxifylline. Hum Reprod 13:911–915CrossRefPubMedGoogle Scholar
  6. Centola GM, Cartie RJ, Cox C (1995) Differential response of human sperm to varying concentrations of pentoxifylline with demonstration of toxicity. J Androl 16:136–142PubMedGoogle Scholar
  7. Dimitriadis F, Giannakis D, Pardalidis N, Zikopoulos K, Paraskevaidis E, Giotitsas N, Kalaboki V, Tsounapi P, Baltogiannis D, Georgiou I (2008) Effects of phosphodiesterase 5 inhibitors on sperm parameters and fertilizing capacity. Asian J Androl 10:115–133CrossRefPubMedGoogle Scholar
  8. Esteves SC (1998) Cryopreservation of human spermatozoa with pentoxifylline improves the post-thaw agonist-induced acrosome reaction rate. Hum Reprod 13:3384–3389CrossRefPubMedGoogle Scholar
  9. Fisch JD (1998) Enhancement of motility and acrosome reaction in human spermatozoa: differential activation by type-specific phosphodiesterase inhibitors. Hum Reprod 13:1248–1254CrossRefPubMedGoogle Scholar
  10. Gradil CM, Ball BA (2000) The use of pentoxifylline to improve motility of cryopreserved equine spermatozoa. Theriogenology 54:1041–1047CrossRefPubMedGoogle Scholar
  11. Henkel RR, Schill WB (2003) Sperm preparation for ART. Reprod Biol Endocrinol 1:108CrossRefPubMedGoogle Scholar
  12. Kay VJ, Coutts JRT, Robertson L (1994) Andrology: effects of pentoxifylline and progesterone on human sperm capacitation and acrosome reaction. Hum Reprod 9:2318–2323PubMedGoogle Scholar
  13. Kinutani M (1999) Effects of pentoxifylline on sperm motion characteristics in normozoospermic men defined by a computer-aided sperm analysis. Hiroshima J Med Sci 48:117–122PubMedGoogle Scholar
  14. Kovacic B, Vlaisavljevic V, Reljic M (2006) Clinical use of pentoxifylline for activation of immotile testicular sperm before ICSI in patients with azoospermia. J Androl 27:45–52CrossRefPubMedGoogle Scholar
  15. Matson PL, Yovich JM, Edirisinghe WR, Junk SM, Yovich JL (1995) An argument for the past and continued use of pentoxifylline in assisted reproductive technology. Hum Reprod 10:67–71CrossRefPubMedGoogle Scholar
  16. Maxwell WMC, Robinson SJ, Roca J, Molinia FC, Sanchez-Partida LG, Evans G (1995) Motility, acrosome integrity and fertility of frozen ram spermatozoa treated with caffeine, pentoxifylline, cAMP, 2-deoxyadenosine and kallikrein. Reprod Fertil Dev 7:1081–1088CrossRefPubMedGoogle Scholar
  17. Mortimer ST, Maxwell WMC (2004) Effect of medium on the kinematics of frozen–thawed ram spermatozoa. Reproduction 127:285–291CrossRefPubMedGoogle Scholar
  18. Nassar A, Mahony M, Blackmore P, Morshedi M, Ozgur K, Oehninger S (1998) Increase of intracellular calcium is not a cause of pentoxifylline-induced hyperactivated motility or acrosome reaction in human sperm. Fertil Steril 69:748CrossRefPubMedGoogle Scholar
  19. Negri P, Grechi E, Tomasi A, Fabbri E, Capuzzo A (1996) Effectiveness of pentoxifylline in semen preparation for intrauterine insemination. Hum Reprod 11:1236–1239PubMedGoogle Scholar
  20. Numabe T, Oikawa T, Kikuchi T, Horiuchi T (2001) Pentoxifylline improves in vitro fertilization and subsequent development of bovine oocytes. Theriogenology 56:225–233CrossRefPubMedGoogle Scholar
  21. Okada H, Tatsumi N, Kanzaki M, Fujisawa M, Arakawa S, Kamidono S (1997) Formation of reactive oxygen species by spermatozoa from asthenospermic patients: response to treatment with pentoxifylline. J Urol 157:2140–2146CrossRefPubMedGoogle Scholar
  22. Stanic P, Sonicki Z, Suchanek E (2002) Effect of pentoxifylline on motility and membrane integrity of cryopreserved human spermatozoa. Int J Androl 25:186–190CrossRefPubMedGoogle Scholar
  23. Tarlatzis BC, Kolibianakis EM, Bontis J, Tousiou M, Lagos S, Mantalenakis S (1995) Effect of pentoxifylline on human sperm motility and fertilizing capacity. Sys Biol Reprod Med 34:33–42CrossRefGoogle Scholar
  24. Terriou P, Hans E, Giorgetti C, Spach JL, Salzmann J, Urrutia V, Roulier R (2000) Pentoxifylline initiates motility in spontaneously immotile epididymal and testicular spermatozoa and allows normal fertilization, pregnancy, and birth after intracytoplasmic sperm injection. J Asisst Reprod Genet 17:194–199CrossRefGoogle Scholar
  25. Tournaye H, Devroey P, Camus M, Van Der Linden M, Janssens R, Van Steirteghem A (1995) Use of pentoxifylline in assisted reproductive technology. Hum Reprod 10:72–72PubMedGoogle Scholar
  26. Verstegen J, Iguer-Ouada M, Onclin K (2002) Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 57:149–179CrossRefPubMedGoogle Scholar
  27. Yogev L, Gamzu R, Botchan A, Homonnai ZT, Amit A, Lessing JB, Paz G, Yavetz H (1995) Pentoxifylline improves sperm binding to the zona pellucida in the hemizona assay. Fertil Steril 64:146–149PubMedGoogle Scholar
  28. Yunes R, Fernández P, Doncel GF, Acosta AA (2005) Cyclic nucleotide phosphodiesterase inhibition increases tyrosine phosphorylation and hyper motility in normal and pathological human spermatozoa. Biocell 29:287–293PubMedGoogle Scholar
  29. Zhang X, Sharma RK, Agarwal A, Falcone T (2005) Effect of pentoxifylline in reducing oxidative stress-induced embryotoxicity. J Asisst Reprod Genet 22:415–417CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2009

Authors and Affiliations

  • Hossein Hassanpour
    • 1
  • Pezhman Mirshokraei
    • 2
  • Parviz Tajik
    • 3
  • Abbas Haghparast
    • 1
  1. 1.Department of Basic Sciences, Faculty of Veterinary MedicineShahrekord UniversityShahrekordIran
  2. 2.Department of Clinical Sciences, Faculty of Veterinary MedicineShahrekord UniversityShahrekordIran
  3. 3.Department of Clinical Sciences, Faculty of Veterinary MedicineUniversity of TehranTehranIran

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