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Comparative Clinical Pathology

, Volume 19, Issue 2, pp 141–145 | Cite as

Studies on the stability of myoglobin in the presences of sodium dodecyl sulfate (SDS) and temperature

  • B. Shareghi
  • H. Nazem
  • Z. Karimi dehkordiEmail author
  • A. Jafari Dehkordi
Original Article

Abstract

This study investigated the stability of myoglobin under different temperatures and sodium dodecyl sulfate (SDS) concentrations. The conformational changes of the protein embedded in SDS with different pH (2, 4.5, and 7.4) and temperature (from 30°C to 100°C) were evaluated using absorption and fluorescence spectroscopy methods. Results indicated that increasing the SDS concentration increased the stability of myoglobin at pH 2 and 4.5, while pH 7.4 had a negative influence on the stability of myoglobin processes. Fluorescence intensities decreased at 50°C and 60°C in pH 2, while they increased at 30°C, 40°C, and 50°C in pH 7.4. The intensity of fluorescence at pH 4.5 remained constant without detectable changes throughout the experiment. In conclusion, increasing pH increased T m occurred, and therefore, the stability of native myoglobin also increased. Myoglobin denaturation with SDS led to an increase in the myoglobin stability at pH 2 and 4.5 and a decrease of the myoglobin stability at pH 7.4.

Keywords

Myoglobin Stability SDS and temperature Temperature 

Notes

Acknowledgment

The authors greatly appreciate the support of this research by the Payamnoor University of Tehran, Iran. The authors thank Zahra Hashemi and Azardokht Reeisi for their help in the biochemistry laboratory.

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Copyright information

© Springer-Verlag London Limited 2009

Authors and Affiliations

  • B. Shareghi
    • 1
  • H. Nazem
    • 2
  • Z. Karimi dehkordi
    • 2
    Email author
  • A. Jafari Dehkordi
    • 3
  1. 1.Science FacultyShahrekord UniversityShahrekordIran
  2. 2.Department of Biochemistry, Science FacultyPayamnoor UniversityTehranIran
  3. 3.Department of Large Animal Internal Medicine, Veterinary FacultyShahrekord UniversityShahrekordIran

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