Serial blood cultures from canine stored whole blood held at room temperature for 24 h
Abstract
To determine if 30- to 35-day-old units of canine stored whole blood held at room temperature over 24 h will reveal bacterial contamination when exposed to repeated sampling. The design of the study is prospective, controlled. Ten units of stored whole blood from ten client owned volunteer blood donor dogs were used. A sterile blood bag spike was inserted aseptically as a port for repeated sampling for culture into ten separate units of 30- to 35-day-old canine stored whole blood held at room temperature for 24 h. Aerobic and anaerobic bacterial culture samples were collected every 4 h over 24 h. No bacterial growth was detected in any sample at any time point from the ten bags used. Canine stored whole blood, collected and stored for 30 to 35 days according to accepted transfusion guidelines, can be aseptically spiked and held at room temperature for 24 h without evidence of bacterial contamination or proliferation.
Keywords
Blood transfusion Bacterial contamination Canine Blood culturesReferences
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