Comparative Clinical Pathology

, 15:181 | Cite as

The susceptibility to amphotericin B-induced hemolysis of erythrocytes from women with breast cancer before and during therapy

  • Agnieszka Knopik-Skrocka
  • Józef Bielawski
  • Zygmunt Kopczyński
  • Sylwia Grodecka-Gazdecka
  • Piotr Tomczak
  • Robert Gryczka
  • Małgorzata Mazur-Roszak
  • Halina Kęsa
Original Article
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Abstract

The kinetics of hemolysis induced by amphotericin B in erythrocytes obtained from women with breast cancer before and during therapy (surgical operation, chemotherapy) was investigated. The changes in absorbance of erythrocyte suspension were monitored by absorption spectrophotometric methods at λ=590 nm. The kinetics of hemolysis induced by amphotericin B in erythrocytes of patients before operation or chemotherapy is the permeability type and is not changed during therapy. The process is similar to that observed in erythrocytes from healthy women. There is no statistical difference between the resistance to amphotericin B of erythrocytes obtained from women with breast cancer before treatment and the resistance obtained for healthy women. The resistance is independent of the cancer stage. Significant increase in the resistance to amphotericin B is found after surgical operation (P<0.01). The increase in resistance to amphotericin B is correlated with the decrease of erythrocyte number, hemoglobin content, and hematocrit. Chemotherapy does not change the resistance of erythrocytes to amphotericin B. The osmotic fragility of patient erythrocytes does not show any significant difference to that obtained for healthy women. No correlation between the osmotic fragility and the resistance of erythrocytes to amphotericin B was found. This indicates that erythrocyte resistance to amphotericin B is not determined by volume and membrane surface area.

Keywords

Amphotericin B Erythrocyte Membrane Hemolysis Breast cancer 
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Notes

Acknowledgements

The authors acknowledge the financial support of the study by grant of A. Mickiewicz University of Poznań and University of Medical Sciences of Poznań.

References

  1. Bagiński M, Resat H, Borowski E (2002) Comparative molecular dynamics simulations of amphotericin B-cholesterol/ergosterol membrane channels. Biochim Biophys Acta 1567:63–78PubMedCrossRefGoogle Scholar
  2. Bielawski J (1990) Two types of hemolytic activity of detergents. Biochim Biophys Acta 1035:214–217PubMedGoogle Scholar
  3. Bolard J, Legrand P, Heitz F, Cybulska B (1991) One-sided action of amphotericin B on cholesterol-containing membranes is determined by its self-association in the medium. Biochemistry 30:5707–5715PubMedCrossRefGoogle Scholar
  4. Brajtburg J, Medoff G, Kobayashi GS, Elberg S, Finegold C (1980) Permeabilizing and hemolytic action of large and small polyene antibiotics on human erythrocytes. Antimicrob Agents Chemother 18:586–592PubMedGoogle Scholar
  5. Charbonneau C, Fournier I, Dufresne S, Barwicz J, Tancrede P (2001) The interactions of amphotericin B with various sterols in relation to its possible use in anticancer therapy. Biophys Chemist 91:125–133CrossRefGoogle Scholar
  6. Clarck MR (1988) Senescence of red blood cells: progress and problems. Physiol Rev 68:03–554Google Scholar
  7. Cybulska B, Bolard J, Seksek O, Czerwiński A, Borowski E (1995) Identification of the structural elements of amphotericin B and other polyene macrolide antibiotics of the heptaene group influencing the ionic selectivity of the permeability pathways formed in the red cell membrane. Biochim Biophys Acta 1240:167–178PubMedCrossRefGoogle Scholar
  8. Danaher PJ, Cao MK, Anstead GM, Dolan MJ, De Witt CC (2004) Reversible dilated cardiomyopathy related to amphotericin B therapy. J Antimicrob Chemother 53:115–117PubMedCrossRefGoogle Scholar
  9. De Kruijff B, Demel RA (1974) Polyene antibiotic-sterol interaction in membranes of Acholeplasma laidlawii cells and lecithin liposomes. III. Molecular structure of the polyene antibiotic-cholesterol complexes. Biochim Biophys Acta 339:57–70PubMedCrossRefGoogle Scholar
  10. De Kruijff B, Gerritsen WJ, Oerlemans A, Demel RA, Van Deenen LM (1974) Polyene antibiotic-sterol interactions in membranes of Acholeplasma laidlawii and lecithin liposomes. I. Specificity of the membrane permeability changes induced by the polyene antibiotics. Biochim Biophys Acta 339:30–43PubMedCrossRefGoogle Scholar
  11. Deray G (2002) Amphotericin B nephrotoxicity. J Antimicrob Chemother 49:37–41PubMedGoogle Scholar
  12. Hartsel S, Wieland T (2003) Amphotericin B binds to amyloid fibrils and delays their formation: a therapeutic mechanism? Biochemistry 42:6228–6233PubMedCrossRefGoogle Scholar
  13. Honda K, Ishiko O, Tatsuta I, Deguchi M, Hirai K, Nakata S, Sumi T, Yasui T, Ogita S (1995) Anemia-inducing substance from plasma of patients with advanced malignant neoplasms. Cancer Res 55:3623–3628PubMedGoogle Scholar
  14. Houdai T, Matsuoka S, Matsumori N, Murata M (2004) Membrane-permeabilizing activities of amphidinol 3, polyene-polyhydroxy antifungal from a marine dinoflagellate. Biochim Biophys Acta 1667:91–100PubMedCrossRefGoogle Scholar
  15. Ishiko O, Sugawa T, Tatsuta I, Shimura K, Naka K, Deguchi M, Umesaki N (1987) Anemia-inducing substance (AIS) in advanced cancer: inhibitory effect of AIS on the function of erythrocytes and immunocompetent cells. Jpn J Cancer Res 78:596–606PubMedGoogle Scholar
  16. King CT, Rogers PD, Cleary JD, Chapman SW (1998) Antifungal therapy during pregnancy. Clin Infect Dis 27:1151–1160PubMedCrossRefGoogle Scholar
  17. Knopik-Skrocka A, Bielawski J (2002) The mechanism of the hemolytic activity of polyene antibiotics. Cell Mol Biol Lett 7:31–48PubMedGoogle Scholar
  18. Knopik-Skrocka A, Bielawski J (2005) Differences in amphotericin B-induced hemolysis between human erythrocytes obtained from male and female donors. Biol Lett 42:49–60Google Scholar
  19. Knopik-Skrocka A, Bielawski J, Głb M, Klafaczyńska A, Wulkiewicz M (2003) A kinetics study of pig erythrocyte hemolysis induced by polyene antibiotics. Cell Mol Biol Lett 8:439–454PubMedGoogle Scholar
  20. Kopczyński Z, Kuźniak J, Thielemann A (2001) Ocena składu fosfolipidów w błonie erytrocytów u chorych na raka piersi. Diagn Lab 37:295–302Google Scholar
  21. Kotler-Brajtburg J, Medoff G, Kobayashi GS, Bogss S, Schlessinger D, Pandey R, Rinehart KL (1979) Classification of polyene antibiotics according to chemical structure and biological effects. Antimicrob Agents Chemother 15:716–722PubMedGoogle Scholar
  22. Krishan A, Saurteig A, Gordon K (1985) Effect of amphotericin B on adriamycin transport in P388 cells. Cancer Res 45:4097–4102PubMedGoogle Scholar
  23. Luber AD, Maa L, Lam M, Guglielmo BJ (1999). Risk factors for amphotericin B-induced nephrotoxicity. J Antimicrob Chemother 43:267–271PubMedCrossRefGoogle Scholar
  24. Masuda H, Tanaka T, Kido A, Kusaba J (1991) Potentation of cisplatin against sensitive and resistant human ovarian cancer cell lines by amphotericin B. Cancer J 4:119–124Google Scholar
  25. Nowicki S, Stefanowski M (1963) In: Zarys chirurgii (ed) Utrata krwi i cieczy ustrojowych. PZWL, Warsaw, pp 152–154Google Scholar
  26. Singh W, Subramaniam S, Shyama S, Jagadeesan M, Devi C (1996) Changes in erythrocyte membrane lipids in breast cancer after radiotherapy and chemotherapy. Chemotherapy 42:65–70CrossRefGoogle Scholar
  27. Teerlink T, De Kruijff B, Demel R (1980) The action of pimaricin, etruscomycin and amphotericin B on liposomes with varying sterol content. Biochim Biophys Acta 599:484–492PubMedCrossRefGoogle Scholar
  28. Torliński L, Karoń H (1972) Trwałoś osmotyczna krwinek czerwonych szczurów z przeszczepionym wtrobiakiem Morrisa. Pat Pol 23:55–60Google Scholar
  29. Vanden Bossche H (1995) Chemotherapy of human fungal infections. In: Lyrr H (ed) Modern selective fungicides. Properties, applications, mechanism of action. Gustav Fisher Verlag, Jena, pp 431–484Google Scholar
  30. Vido A, Cavalcanti T, Guimaraes F, Vieira-Matos A, Rettori O (2000) The hemolytic component of cancer anemia: effects of osmotic and metabolic stress on the erythrocytes of rats bearing multifocal inoculations of the Walker 256 tumor. Braz J Med Biol Res 33:815–822PubMedCrossRefGoogle Scholar
  31. Witkowski JM (1993) Zmiany w błonie jako składnik procesu starzenia się. Post Biol Kom 20:111–132Google Scholar
  32. Zotchev S (2003) Polyene macrolide antibiotics and their applications in human therapy. Curr Med Chem 10:1241–1253Google Scholar
  33. Zumbuehl A, Jeannerat D, Martin SE, Sohrmann M, Stano P, Vigassy T, Clark DD, Hussey L, Peter M, Peterson BR, Pretsch E, Walde P, Carrira EM (2004) An amphotericin B-fluorescein conjugate as a powerful probe for biochemical studies of the membrane. Angew Chem Int Ed Engl 43:5428CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2006

Authors and Affiliations

  • Agnieszka Knopik-Skrocka
    • 1
  • Józef Bielawski
    • 1
  • Zygmunt Kopczyński
    • 2
  • Sylwia Grodecka-Gazdecka
    • 3
  • Piotr Tomczak
    • 4
  • Robert Gryczka
    • 3
  • Małgorzata Mazur-Roszak
    • 4
  • Halina Kęsa
    • 1
  1. 1.Department of Cell BiologyAdam Mickiewicz UniversityPoznańPoland
  2. 2.Department of Diagnostics Laboratory, Chair of OncologyUniversity of Medical SciencesPoznańPoland
  3. 3.Department of Surgery, Chair of OncologyUniversity of Medical SciencesPoznańPoland
  4. 4.Department of Chemotherapy, Chair of OncologyUniversity of Medical SciencesPoznańPoland

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