Comparative Clinical Pathology

, Volume 14, Issue 3, pp 149–154 | Cite as

Effects of preweaning parenteral supplementation of vitamin E and selenium on hematology, serum proteins, and weight gain in dairy calves

  • M. Mohri
  • H. A. Seifi
  • J. Khodadadi
Original Article


The beneficial effects of nutritional or parenteral administration of vitamin E (vit E) and selenium (Se) have not been universal among studies, and potentially confounding factors, such as Se or vit E status at the time of administration, duration and dose, and preexisting incidence of disease within a herd before administration, can all affect the outcome of treatment. Forty calves were used in the present study. The animals were divided into two groups (test, n=20; control, n=20). The two groups of calves were homogeneous for parity of dams, sex, and month of birth. In the test group, vit E and Se were injected at a dose of 300 U vit E (?-tocopherol acetate) plus 6 mg Se (sodium selenite) per 45 kg body weight (vit E + Se, BASF, Spain) at 24--48 h and 14 days after birth. Sampling was conducted from the jugular vein between 24 and 48 h after birth (before drug administration in the test group) and days 7, 14, 21, and 28, and erythrogram, leukogram, total serum protein, and fibrinogen amounts were determined. The amounts of beta and gamma globulins and the osmotic fragility of red blood cells were measured only at the end of the trial. Calves were weighed after birth, at days 7, 14, 21, and 28, and after 3 months. A general linear model showed significant changes for packed cell volume (PCV), fibrinogen, neutrophil, lymphocyte, and monocyte levels in the control group and total protein and lymphocyte in the test group, but interaction between sampling time and group for all parameters was not significant. White blood cells and hemoglobin levels at the third week and PCV and beta globulin amounts at the fourth week of study showed significant differences between groups (p<0.05). There were no significant differences for other measured parameters and weight gain between groups.


Selenium Vitamin E Calf Hematology Performance 


  1. Arechiga CF, Vazquez-Flores S, Ortiz O, Hernandez-Ceron J, Porras A, McDowell LR, Hansen PJ (1998) Effect of injection of ?-carotene or vitamin E and selenium on fertility of lactating dairy cows. Theriogenology 50:65--76CrossRefPubMedGoogle Scholar
  2. Bednarek D, Kondracki M, Cakala S (1996) Untersuchungen uber den einfluss von selen und vitamin E auf rotes und weisses blutbild, serum konzentration einiger mineralstoffe und spurenelemente sowie immunologische parameter beim kalb. Dtsch Tierarztl Wochenschr 103:457--459PubMedGoogle Scholar
  3. Castellan DM, Mass JP, Gardner IA, Oltjen JW, Sween ML (1999) Growth of suckling beef calves in response to parenteral administration of selenium and the effect of dietary protein provided to their dams. J Am Vet Med Assoc 214:816--821PubMedGoogle Scholar
  4. Cay M, Naziroglu M (1999) Effects of intraperitoneally-administered vitamin E and selenium on the blood biochemical and haematological parameters in rats. Cell Biochem Funct 17:143--148CrossRefPubMedGoogle Scholar
  5. Chanarin I (1989) Laboratory haematology: an account of laboratory techniques. Churchill Livingston, New York, pp 94--95Google Scholar
  6. Dacie JV, Lewis SM (1984) Practical haematology. Churchill Livingston, Edinburgh, pp 22--49Google Scholar
  7. Di Mascio P, Murphy ME, Sies H (1991) Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. Am J Clin Nutr 53:194S--200SPubMedGoogle Scholar
  8. Enjalbert F, Lebreton P, Salat O, Schelcher F (1999) Effects of pre- or postpartum selenium supplementation on selenium status in beef cows and their calves. J Anim Sci 77:223--229PubMedGoogle Scholar
  9. Erskine RJ, Bartlett PC, Herdt T, Gaston P (1997) Effects of parenteral administration of vitamin E on health of periparturient dairy cows. J Am Vet Med Assoc 211:466--469PubMedGoogle Scholar
  10. Gunter SA, Beck PA, Philips JM (2003) Effects of supplementary selenium source on the performance and blood measurements in beef cows and their calves. J Anim Sci 81:856--864PubMedGoogle Scholar
  11. Hidiroglou M, Batra TR, Laflamme LF, Markham F (1992) Possible roles of vitamine E in immune response of calves. Int J Vitam Nutr Res 62:308--311PubMedGoogle Scholar
  12. Hidiroglou M, Batra TR, Ivan M, Markham F (1995) Effects of supplemental vitamins E and C on the immune responses of calves. J Dairy Sci 78:1578--1583PubMedGoogle Scholar
  13. Hogan JS, Weiss WP, Smith KL (1993) Role of vitamin E and selenium in host defence against mastitis. J Dairy Sci 76:2795--2803PubMedCrossRefGoogle Scholar
  14. Izadyar F (1987) Serum creatin kinase and whole blood selenium concentrations of clinically normal sheep of various regions of Iran. D.V.M. thesis, no. 1709, School of Veterinary Medicine, Tehran UniversityGoogle Scholar
  15. Jain NC (1986) Schalm's veterinary hematology. Lea and Febiger, Philadelphia, pp 66--67Google Scholar
  16. Koller LD, Exone JH (1986) The two faces of selenium deficiency and toxicity are similar in animals and man. Can J Vet Res 50, 297--306PubMedGoogle Scholar
  17. Kramer JW (2000) Normal hematology of cattle, sheep and goats. In: Feldman BF, Zinkle JG, Jain NC (eds) Schalm's veterinary hematology. Lippincott Williams and Wilkins, Philadelphia, pp 1077--1078Google Scholar
  18. Lacetera N, Bernabuci U, Ronchi B, Nardone A (1996) Effects of selenium and vitamin E administration during a late stage of pregnancy on colostrum and milk production in dairy cows, and on passive immunity and growth of their offspring. Am J Vet Res 57:1776?1780PubMedGoogle Scholar
  19. McCay PB, King MM (1980) Vitamin E: its role as a biologic free radical scavenger and its relationship to the microsomal mixed-function oxidase system. In: Machlin LJ (ed) Vitamin E: a comprehensive treatise. Marcel Dekker, New York, pp 289--317Google Scholar
  20. Miller JK, Mueller FJ, Thomas DG, Madsen FC (1991) Vitamin E and reproduction in dairy cows. BASF Proceedings 159Google Scholar
  21. National Research Council (2001) Nutrient requirements of dairy cattle. National Academy of Sciences, Washington, DCGoogle Scholar
  22. Ndiweni N, Finch JM (1996) Effects of in vitro supplementation with ?-tocopherol and selenium on bovine neutrophil functions: implication for resistance to mastitis. Vet Immunol Immunopathol 51:67--78CrossRefPubMedGoogle Scholar
  23. Nemec M, Hidiroglou M, Nielsen K, Proulx L (1990) Effect of vitamin E and selenium supplementation on some immune parameters following vaccination against brucellosis in cattle. J Anim Sci 68:4303--4309PubMedGoogle Scholar
  24. Maas J, Peauroi JR, Tonjes T, Karlonas J, Galey FD, Bin H (1993) Intramuscular selenium: administration in selenium-deficient cattle. J Vet Intern Med 7:342--348PubMedGoogle Scholar
  25. Pehrson B, Ortman K, Madjid N, Trafikowska U (1999) The influence of dietary selenium as selenium yeast or sodium selenite on the concentration of selenium in the milk of suckler cows and on the selenium status of their calves. J Anim Sci 77:3371--3376PubMedGoogle Scholar
  26. Reddy PG, Morrill JL, Frey RA, Morrill MB, Minocha HC, Galitzer SJ, Dayton AD (1985) Effects of supplemental vitamin E on the performance and metabolic profiles of dairy calves. J Dairy Sci 68:2259--2266PubMedGoogle Scholar
  27. Reddy PG, Morrill JL, Minocha HC, Morrill MB, Dayton AD, Frey RA (1986) Effect of supplemental vitamin E on the immune system of calves. J Dairy Sci 69:164--171PubMedGoogle Scholar
  28. Reddy PG, Morrill JL, Frey RA (1987) Vitamin E requirements of dairy calves. J Dairy Sci 70:123--129PubMedGoogle Scholar
  29. Rivera JD, Duff GC, Galyean DA, Walker DA, Nunnery GA (2002) Effects of supplemental vitamin E on performance, health and humoral immune response of beef cattle. J Anim Sci 80:933--941PubMedGoogle Scholar
  30. Stowe HD, Thomas JW, Johnson T, Marteniuk JV, Morrow DA, Ullrey DE (1988) Responses of dairy cattle to long-term and short-term supplementation with oral selenium and vitamin E. J Dairy Sci 71:1830--1839PubMedCrossRefGoogle Scholar
  31. Swecker WS Jr, Eversole DE, Thatcher CD, Blodgett DL, Schuring GG, Meldum JB (1989) Influence of supplemental selenium on humoral responses in weaned beef calves. Am J Vet Res 50:1760?1763PubMedGoogle Scholar
  32. Ullrey DE, Brady PS, Whetter PA, Ku PK, Magee WT (1977) Selenium supplementation of diets for sheep and beef cattle. J Anim Sci 45:559?565Google Scholar
  33. Weiss WP, Colenbrander VF, Cunningham MD, Callahan CJ (1983) Selenium/Vitamin E: role in disease prevention and weight gain of neonatal calves. J Dairy Sci 66:1101--1107PubMedCrossRefGoogle Scholar
  34. Wright CL, Corah LR, Stokka GL, Blecha F (1997) The effects of pre-weaning vitamin E and selenium supplementation on the performance, serum metabolite concentrations and antibody titers of stressed beef calves (abstract). J Anim Sci 75(Suppl 1):266PubMedGoogle Scholar

Copyright information

© Springer-Verlag London Limited 2005

Authors and Affiliations

  1. 1.Department of Clinical SciencesSchool of Veterinary Medicine, Ferdowsi University of MashhadMashhadIran

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