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Mycorrhiza

, Volume 10, Issue 3, pp 107–114 | Cite as

Anatomical and morphological characterization of mycorrhizas of five strains of Tuber borchii Vittad

  • G. Giomaro
  • A. Zambonelli
  • D. Sisti
  • M. Cecchini
  • V. Evangelista
  • V. Stocchi
ORIGINAL PAPER

Abstract

Tilia platyphyllos Scop. plantlets were inoculated in vitro with five Tuber borchii Vittad. strains (1BO, 17BO, 43BO, 71BO and 10RA) to test their intraspecific variability. The ability of the strains to form mycorrhizas varied, with the mean degree of ectomycorrhizal infection ranging from 50.6% (for 1BO) to 82.1% (for 10RA). The anatomical/morphological characteristics of the resulting mycorrhizas were determined. Although the morphological features of the mycorrhizas and the characteristics of the cystidia were similar for all strains tested, differences were found in the anatomical features of the mantle. The form of the mantle cells was examined in the surface and inner layers (6 and 12 μm deep, respectively) by both conventional and confocal microscopy. These cells were polygonal in 1BO, and 71BO, epidermoid in 43BO and intermediate in 17BO and 10RA. The structure of the mantle also varied and thus provided little information with which to identify T. borchii mycorrhizas.

KeywordsTuber borchii Ectomycorrhiza Tilia platyphyllos Mantle features 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • G. Giomaro
    • 1
  • A. Zambonelli
    • 2
  • D. Sisti
    • 1
  • M. Cecchini
    • 1
  • V. Evangelista
    • 3
  • V. Stocchi
    • 4
  1. 1.Istituto e Orto Botanico, Cattedra di Botanica, Università degli Studi di Urbino, Via Bramante 28, 61029 Urbino, Italy e-mail: g.giomaro@uniurb.it Tel.: +39-722-2428 Fax: +39-722-4092IT
  2. 2.Dipartimento di Protezione e Valorizzazione Agroalimentare, Sezione di Patologia Vegetale, Università degli Studi di Bologna, Via Filippo Re 8, 41026 Bologna, ItalyIT
  3. 3.Istituto di Biofisica, CNR, Via S. Lorenzo 26, 56127 Pisa, ItalyIT
  4. 4.Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Via Saffi 2, 61029 Urbino, ItalyIT

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