Comparative measurements of arbuscular mycorrhizal fungal responses to agricultural management practices
Arbuscular mycorrhizal (AM) fungi are considered to be a key group of soil organisms for assessments of soil biological properties and developing relationships among crop production management practices, soil properties, crop performance, and ecosystem services. In a field study of cover crop treatments established during the transition from small grains to corn (Zea mays L), we assessed multiple measures of AM fungal responses to the management treatments: soil propagule numbers, biomass via lipid biomarkers, and root colonization extent. Our objectives were to determine response variables that reliably distinguished cover crop treatments and formed consistent relationships with grain yield, plant biomass, and mineral nutrient concentrations of the following corn crop. The number of soil AM fungal propagules and amount of the NLFA biomarker C16:1cis11 measured on fall-collected soils most consistently and significantly responded to fall cover crop treatments. Neither of these measures of soil inoculum potential was strongly related to measures of crop performance. The PLFA biomarker C16:1cis11 was marginally responsive to cover crop but did not strongly relate to crop performance parameters. Corn root colonization by AM fungi was not significantly affected by cover crop treatment, but significant negative relationships were found between root colonization and grain N concentration and plant biomass at maturity. In contrast, a significant positive relationship between root colonization and plant N concentration at the 6-leaf stage was found. Understanding the relative effectiveness and limitations of AM fungal response variables will inform their application in field studies of agricultural management practices.
KeywordsAgriculture Arbuscular mycorrhizae Cover crops Maize (Zea mays L.) Phosphorus
The research was conducted with the technical support of Sharon Nichols, Amy Christie, Kurt Dagel, and Chris Nelson.
This work was partially funded by the South Dakota Corn Utilization Council.
Compliance with ethical standards
Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.
- Bryce SA, Omernik JM, Pater DE, Ulmer M, Schaar J, Freeouf J, Johnson R, Kuck P, Azevedo SH (1996) Ecoregions of North Dakota and South Dakota (map scale 1:1,500,000). U.S. Geological Survey, RestonGoogle Scholar
- Creamer R, Hannula S, Van Leeuwen J, Stone D, Rutgers M, Schmelz R, De Ruiter P, Hendriksen NB, Bolger T, Bouffaud M-L (2016) Ecological network analysis reveals the inter-connection between soil biodiversity and ecosystem function as affected by land use across Europe. Appl Soil Ecol 97:112–124CrossRefGoogle Scholar
- Dai M, Hamel C, Bainard LD, Arnaud MS, Grant CA, Lupwayi NZ, Malhi SS, Lemke R (2014) Negative and positive contributions of arbuscular mycorrhizal fungal taxa to wheat production and nutrient uptake efficiency in organic and conventional systems in the Canadian prairie. Soil Biol Biochem 74:156–166CrossRefGoogle Scholar
- Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Circular 347 (2nd edition). California Agricultural Experimental Station, BerkeleyGoogle Scholar
- Jansa J, Wiemken A, Frossard E (2006) The effects of agricultural practices on arbuscular mycorrhizal fungi. Geol Soc Lond Spec Publ 266:89–115Google Scholar
- Jeffries P, Gianinazzi S, Perotto S, Turnau K, Barea J-M (2003) The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biol Fertil Soils 37:1–16Google Scholar
- Pinkart HC, Ringelberg DB, Piceno YM, MacNaughton SJ, White DC (2002) Biochemical approaches to biomass measurements and community structure analysis. In: Hurst CJ, Crawford RL, Knudsen GR, McInerney MJ, Stetzenbach LD (eds) Manual of environmental microbiology. ASM Press, Washington, D.C., pp 101–113Google Scholar
- Smith SE, Read DJ (2008) Mycorrhizal Symbiosis, 3rd edn. Academic, LondonGoogle Scholar
- Sylvia DM (1994) Vesicular-arbuscular mycorrhizal fungi. In: Weaver RW, Angle S, Bottomley P, Bezdicek D, Smith S, Tabatabai A, Wollum A (eds) Methods of soil analysis, part 2. Microbiological and biochemical properties, vol 5. Soil Science Society of America, Madison, pp 351–378Google Scholar
- Woomer PL (1994) Most probable number counts. In: Weaver RW, Angle S, Bottomley P, Bezdicek D, Smith S, Tabatabai A, Wollum A (eds) Methods of soil analysis, part 2. Microbiological and biochemical properties, vol 5. Soil Science Society of America, Madison, pp 59–80Google Scholar