Advertisement

Mycorrhiza

, Volume 27, Issue 7, pp 709–718 | Cite as

Influence of host tree species on isolation and communities of mycorrhizal and endophytic fungi from roots of a tropical epiphytic orchid, Dendrobium sinense (Orchidaceae)

  • Xiaoming Wang
  • Yijia Li
  • Xiqiang Song
  • Qianwan Meng
  • Jie Zhu
  • Ying Zhao
  • Wengang Yu
Original Article

Abstract

Most studies on the host preference of orchids have focused on the association between orchids and host characteristics, but little is known about the differences of mycorrhizal and endophytic fungal communities in epiphytic orchids growing on different host tree species. We selected Dendrobium sinense, a tropical epiphytic orchid, to determine if fungal endophytes from the roots of D. sinense were preferentially correlated with host tree species. Fifty-six fungal operational taxonomic units (OTUs) from 36 host trees were identified. The results indicated that the species richness and diversity of mycorrhizal and endophytic fungal communities isolated from D. sinense roots were strongly influenced by host tree species. Both species richness and diversity indices showed that D. sinense roots on Syzygium buxifolium harbored the most diverse and abundant endophytic fungi. Species of Tulasnellaceae were dominant on S. buxifolium and Rhododendron moulmainense but infrequent on Cyclobalanopsis disciformis and Podocarpus neriifolius. Our results provide evidence for distinct mycorrhizal and endophytic fungal communities on different host tree species. Further research focusing on fungi-orchid-host preference could be conducted to increase our understanding for the in situ conservation of epiphytic orchids.

Keywords

Host tree Epiphytic orchid Mycorrhizal fungi Endophytic fungi 

Notes

Acknowledgements

This research was financially supported by the Special Fund for Agro-scientific Research in the Public Interest (201303117) and by the Excellent Graduate Dissertation Development Scheme of Hainan University (2014), and partially supported by the National Natural Science Foundation of China (31160178). We thank Shanjiang Qi for the help on field sampling, and thank Prof. Peng Ling for the useful comments on this manuscript.

Supplementary material

572_2017_787_MOESM1_ESM.pdf (104 kb)
Figure S1 (PDF 104 kb)
572_2017_787_MOESM2_ESM.pdf (311 kb)
Figure S2 (PDF 311 kb)
572_2017_787_MOESM3_ESM.pdf (330 kb)
Figure S3 (PDF 330 kb)
572_2017_787_MOESM4_ESM.pdf (335 kb)
Figure S4 (PDF 334 kb)
572_2017_787_MOESM5_ESM.docx (15 kb)
Table S1 (DOCX 14 kb)
572_2017_787_MOESM6_ESM.docx (18 kb)
Table S2 (DOCX 18 kb)
572_2017_787_MOESM7_ESM.docx (19 kb)
Table S3 (DOCX 19 kb)

References

  1. Alexander C, Hadley G (1983) Variation in symbiotic activity of Rhizoctonia isolates from Goodyera repens mycorrhizas. Trans British Mycol Soc 80:99–106CrossRefGoogle Scholar
  2. Arditti J, Ernst R, Yam TW, Glabe C (1990) The contributions of orchid mycorrhizal fungi to seed germination: a speculative review. Lindleyana 5:249–255Google Scholar
  3. Bailarote BC, Lievens B, Jacquemyn H (2012) Does mycorrhizal specificity affect orchid decline and rarity? Am J Bot 99(10):1655–1665CrossRefPubMedGoogle Scholar
  4. Bayman P, Otero JT (2006) Microbial endophytes of orchid roots. In: Schulz B (ed) Microbial root endophytes. Springer, New York, pp 153–173CrossRefGoogle Scholar
  5. Bayman P, Lebrón LL, Tremblay RL, Lodge DJ (1997) Variation in endophytic fungi from roots and leaves of Lepanthes (Orchidaceae). New Phytologist 135 (1):143-149Google Scholar
  6. Bayman P, Gonzalez EJ, Fumero JJ, Tremblay RL (2002) Are fungi necessary? How fungicides affect growth and survival of the orchid Lepanthes rupestris in the field. J Ecol 90:1002–1008CrossRefGoogle Scholar
  7. Boddington M, Dearnaley JDW (2008) Morphological and molecular identification of fungal endophytes from roots of Dendrobium speciosum. Proceedings of the Royal Society of Queensland 114:13–17Google Scholar
  8. Brundrett MC (2002) Coevolution of roots and mycorrhizas of land plants. New Phytol 154:275–304CrossRefGoogle Scholar
  9. Callaway RM, Reinhart KO, Moore GW, Moore DJ, Pennings SC (2002) Epiphyte host preferences and host traits: mechanisms for species-specific interactions. Oecologia 132:221–230CrossRefPubMedGoogle Scholar
  10. Caporaso JG, Kuczynski J, Stombaugh J et al (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7(5):335–336CrossRefPubMedPubMedCentralGoogle Scholar
  11. Chang DCN, Chou LC (2001) Seed germination of Haemaria discolor var. dawsoniana and the use of mycorrhizae. Symbiosis 30:29–40Google Scholar
  12. Chen J, Hu K, Hou X, Guo S (2011) Endophytic fungi assemblages from Dendrobium medicinal plants (Orchidaceae). World J Microbiol Biotehnol 27:1009–1016CrossRefGoogle Scholar
  13. Chen J, Wang H, Guo SX (2012) Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae). Mycorrhiza 22:297–307CrossRefPubMedGoogle Scholar
  14. Crain BJ (2012) On the relationship between bryophyte cover and the distribution of Lepanthes spp. Lankesteriana 12(1):13–18CrossRefGoogle Scholar
  15. Cribb PJ, Lell SP, Dixon KW, Barrett RL (2003) Orchid conservation: a global perspective. In: Dixon KW, Kell SP, Barrett RL, Cribb PJ (eds) Orchid conservation. Natural History Publications, Kota Kinabalu, pp 1–24Google Scholar
  16. Dearnaley JDW, Martos F, Selosse MA (2012) Orchid mycorrhizas: molecular ecology, physiology, evolution and conservation aspects. In: Hock B (ed) Fungal associations. Springer, Berlin, pp 207–230CrossRefGoogle Scholar
  17. Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:2460–2461CrossRefPubMedGoogle Scholar
  18. Frohlich J, Hyde KD, Petrini O (2000) Endophytic fungi associated with palms. Mycol Res 104:1202–1212Google Scholar
  19. Gentry AH, Dodson C (1987) Contribution of non-trees to species richness of atropical rain forest. Biotropica 19:149–156CrossRefGoogle Scholar
  20. Gowland KM, Mathesius U, Clements MA, Nicotra AB (2007) Understanding the distribution of three species of epiphytic orchids in temperate Australian rain forest by investigation of their host and fungal associates. Lankesteriana 7:44–46Google Scholar
  21. Gowland KM, van der Merwe MM, Linde CC, Clements MA, Nicotra AB (2013) The host bias of three epiphytic Aeridinae orchid species is reflected, but not explained, by mycorrhizal fungal associations. Am J Bot 100:764–777CrossRefPubMedGoogle Scholar
  22. Graham RR, Dearnaley JDW (2012) The rare Australian epiphytic orchid Sarcochilus weinthalii associates with a single species of Ceratobasidium. Fungal Divers 54:31–37CrossRefGoogle Scholar
  23. Guo LD, Hyde KD, Liew ECY (2000) Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol 147:617–630CrossRefGoogle Scholar
  24. Harvais G, Raitsakas A (1975) On the physiology of a fungus symbiotic with orchids. Can J Bot 53:144–155CrossRefGoogle Scholar
  25. Herrera H, Valadares R, Contreras D, Bashan Y, Arriagada C (2017) Mycorrhizal compatibility and symbiotic seed germination of orchids from the Coastal Range and Andes in south central Chile. Mycorrhiza 27:175–188CrossRefPubMedGoogle Scholar
  26. Jersakova J, Kindlmann P, Renner SS (2006) Is the colour dimorphism in Dactylorhiza sambucina maintained by differential seed viability instead of frequency-dependent selection? Folia Geobot 41:61–76CrossRefGoogle Scholar
  27. Lugtenberg BJJ, Dekkers LC (1999) What makes Pseudomonas bacteria rhizosphere competent?. Environmental Microbiology 1 (1):9-13Google Scholar
  28. Kress W (1986) The systematic distribution of vascular epiphytes: an update. Selbyana 9:2-22Google Scholar
  29. Magurran AE (1988) Ecological diversity and its measurement. Princeton University Press, PrincetonCrossRefGoogle Scholar
  30. McCormick MK, Jacquemyn H (2014) What constrains the distribution of orchid populations? New Phytol 202:392–400CrossRefGoogle Scholar
  31. Nontachaiyapoom S, Sasirat S, Manoch L (2010) Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand. Mycorrhiza 20:459–471CrossRefPubMedGoogle Scholar
  32. Otero JT, Ackerman JD, Bayman P (2002) Diversity and host specificity of mycorrhizal fungi from tropical orchids. Am J Bot 89:1852–1858CrossRefGoogle Scholar
  33. Otero JT, Mosquera AT, Flanagan NS (2013) Tropical orchid mycorrhizae: potential applications in orchid conservation, commercialization, and beyond. Lankesteriana 13:57–63Google Scholar
  34. Rivera-Orduna FN, Suarez-Sanchez RA, Flores-Bustamante ZR, Gracida-Rodriguez JN, Flores-Cotera LB (2011) Diversity of endophytic fungi of Taxus globosa (Mexican yew). Fungal Diversity 47 (1):65-74Google Scholar
  35. de la Rosa-Manzano E, Andrade JL, Zotz G, Reyes-Garcia C (2014) Epiphytic orchids in tropical dry forests of Yucatan, Mexico—species occurrence, abundance and correlations with host tree characteristics and environmental conditions. Flora 209:100–109CrossRefGoogle Scholar
  36. Sánchez MS, Bills GF, Zabalgogeazcoa I (2008) Diversity and structure of the fungal endophytic assemblages from two sympatric coastal grasses. Fungal Divers 33:87–100Google Scholar
  37. Smith SE, Read DJ (2008) Mycorrhizal symbiosis. Academic Press, LondonGoogle Scholar
  38. Song XQ (2005) Studies on the wild Dendrobium germplasm resources in Hainan Island with special reference to conservation biology of D. sinense. Dissertation, Beijing Forestry University.Google Scholar
  39. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  40. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25(24):4876–4882CrossRefPubMedPubMedCentralGoogle Scholar
  41. Timsina B, Rokaya MB, Munzbergova Z, Kindlmann P, Shrestha B, Bhattarai B, Raskoti BB (2016) Diversity, distribution and host-species associations of epiphytic orchids in Nepal. Biodivers Conserv DOI. doi: 10.1007/s10531-016-1205-8
  42. Westerhuis JA, Hoefsloot HCJ, Smit S, Vis DJ, Smilde AK, Velzen EJJ, Duijnhoven JPM, Dorsten FA (2008) Assessment of PLSDA cross validation. Metabolomics 4(1):81–89CrossRefGoogle Scholar
  43. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar
  44. Wu HZ (2012) Population ecology of Dendrobium sinense (Orchidaceae), an endemic species to Hainan Island. Hainan University, DissertationGoogle Scholar
  45. Wu LS, Han T, Li WC et al (2013) Geographic and Tissue Influences on Endophytic Fungal Communities of Taxus chinensis var. mairei in China. Current Microbiology 66 (1):40-48Google Scholar
  46. Xing YM, Chen J, Cui JL, Chen XM, Guo SX (2011) Antimicrobial activity and biodiversity of endophytic fungi in Dendrobium devonianum and Dendrobium thyrsiflorum from Vietnam. Curr Microbiol 62:1218–1224CrossRefPubMedGoogle Scholar
  47. Xing XK, Gai XG, Liu Q, Hart MM, Guo SX (2015) Mycorrhizal fungal diversity and community composition in a lithophytic and epiphytic orchid. Mycorrhiza 25:289–296CrossRefPubMedGoogle Scholar
  48. Yang FS (2009) Conservation biology on the base of endophytic mycorrhizal fungi of an endangered orchid Dendrobium sinense. Hainan University, DissertationGoogle Scholar
  49. Yuan ZL, Chen YC, Yang Y (2009) Diverse non-mycorrhizal fungal endophytes inhabiting an epiphytic, medicinal orchid (Dendrobium nobile): estimation and characterization. World J Microbiol Biotehnol 25:295–303CrossRefGoogle Scholar
  50. Zettler LW, Piskin KA, Stewart SL, Hartsock JJ, Bowels ML, Bell TJ (2005) Protocorm mycobionts of the federally threatened eastern prairie fringed orchid, Platanthera leucophaea (Nutt.) Lindley, and a technique to prompt leaf elongation in seedlings. Stud Mycol 53:163–171CrossRefGoogle Scholar
  51. Zhu GS, Yu ZN, Gui Y, Liu ZY (2008) A novel technique for isolating orchid mycorrhizal fungi. Fungal Divers 33:123–137Google Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Xiaoming Wang
    • 1
  • Yijia Li
    • 2
  • Xiqiang Song
    • 1
  • Qianwan Meng
    • 1
  • Jie Zhu
    • 1
  • Ying Zhao
    • 1
  • Wengang Yu
    • 1
  1. 1.Institute of Tropical Agriculture and ForestryHainan UniversityHaikouChina
  2. 2.Zhangzhou Institute of TechnologyZhangzhouChina

Personalised recommendations