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Mycorrhiza

, Volume 24, Issue 5, pp 361–368 | Cite as

Influence of phosphorus application and arbuscular mycorrhizal inoculation on growth, foliar nitrogen mobilization, and phosphorus partitioning in cowpea plants

  • Victor Désiré TaffouoEmail author
  • Benard Ngwene
  • Amougou Akoa
  • Philipp Franken
Original Paper

Abstract

The present study was undertaken to evaluate the effects of phosphorus (P) application and arbuscular mycorrhizal (AM) fungi (Funneliformis mosseae) on growth, foliar nitrogen mobilization, and phosphorus partitioning in cowpea (Vigna unguiculata cv. Vita-5) plants. The experiment was conducted in a greenhouse in pots containing a mixture of vermiculite and sterilized quartz sand. Mycorrhizal and non-mycorrhizal cowpea plants were supplied with three levels of soluble P (0.1 (low P), 0.5 (medium P), or 1.0 mM (high P)).

Cowpea plants supplied with low P fertilization showed significantly (p < 0.05) higher root colonization than those with medium and high P fertilization at both the vegetative and pod-filling stages. P uptake and growth parameters of cowpea plants were positively influenced by mycorrhizal inoculation only in the medium P fertilization treatment at the vegetative stage. Lack of these effects in the other treatments may be linked to either a very low P supply (in the low P treatment at the vegetative stage) or the availability of optimal levels of freely diffusible P in the substrate towards the pod-filling stage due to accumulation with time. The N concentration in leaves of all cowpea plants were lower at the pod-filling stage than at the vegetative stage, presumably as a result of N mobilization from vegetative organs to the developing pods. This was however not influenced by AM fungal inoculation and may be a consequence of the lack of an improved plant P acquisition by the fungus at the pod-filling stage.

Keywords

Vigna unguiculata Mycorrhiza Phosphorus fertilization Nitrogen mobilization Phosphorus acquisition 

Notes

Acknowledgments

This research was supported by the TWAS-DFG Cooperation Visits Programme for scientists from sub-Saharan Africa through grant no. 3240249438 to Prof. Dr. Victor Désiré Taffouo and by the Ministries of Consumer Protection, Food and Agriculture of the Federal Republic of Germany, of the Land Brandenburg and of the Land Thüringen. The authors thank Mrs. Susanne Jeserigk and Mrs. Kerstin Schmidt for their excellent technical assistance and the anonymous reviewers for the very valuable comments on the original version of the manuscript.

References

  1. Ahiabor BD, Hirata H (1994) Characteristic responses of three tropical legumes to the inoculation of two species of VAM fungi in Andosol soils with different fertilities. Mycorrhiza 5:63–70CrossRefGoogle Scholar
  2. Ahiabor BDK, Hirata H (2003) Associative influence of soluble phosphate, rock phosphate and arbuscular mycorrhizal fungus on plant growth and phosphorus uptake of three tropical legumes. W Afr J Appl Ecol 4:75–90Google Scholar
  3. Al-Karaki GN, Clark RB (1998) Growth, mineral acquisition, and water use by mycorrhizal wheat grown under water stress. J Plant Nutr 21:263–276CrossRefGoogle Scholar
  4. Ames RN, Bethlenfalvay GJ (1987) Localised increase in nodule activity, but no competitive interaction of cowpea rhizobia due to pre-establishment of vesicular-arbuscular mycorrhiza. New Phytol 106:207–215CrossRefGoogle Scholar
  5. Ames RN, Reid CPP, Porter LK, Cambardella C (1983) Hyphal uptake and transport of nitrogen from two 15 N-labelled sources of Glomus mosseae, a vesicular-arbuscular mycorrhizal fungus. New Phytol 95:381–396CrossRefGoogle Scholar
  6. Asghari HR, Chittleborough DG, Smith FA, Smith SE (2005) Influence of arbuscular mycorrhizal (AM) symbiosis on phosphorus leaching through soil cores. Plant Soil 275:181–193CrossRefGoogle Scholar
  7. Asghari HR, Cavagnaro TR (2011) Arbuscular mycorrhizas enhance plant interception of leached nutrients. Funct Plant Biol 38:219–226CrossRefGoogle Scholar
  8. Azcόn R, Ambrosano E, Charest C (2003) Nutrient acquisition in mycorrhizal lettuce plants under different phosphorus and nitrogen concentrations. Plant Sci 165:1137–1145CrossRefGoogle Scholar
  9. Balzergue C, Puech-Pagès V, Bécard G, Rochange SF (2011) The regulation of arbuscular mycorrhizal symbiosis by phosphate in pea involves early and systemic signalling events. J Exp Bot 62(3):1049–1060PubMedCentralPubMedCrossRefGoogle Scholar
  10. Baon JB, Smith SE, Alston AM, Wheeler RD (1992) Phosphorus efficiency of three cereals as related to indigenous mycorrhizal infection. Aust J Agric Res 43:479–491CrossRefGoogle Scholar
  11. Barea JM, El-Atrach F, Azcόn R (1989) Mycorrhiza and phosphate interactions as affecting plant development, N2-fixation, N-transfer and N-uptake from soil in legume–grass mixtures by using a 15 N dilution technique. Soil Biol Biochem 21(4):581–589CrossRefGoogle Scholar
  12. Barea JM, Toro M, Orozco MO, Campos E, Azcόn R (2002) The application of isotopic (32P and 15 N) dilution techniques to evaluate the interactive effect of phosphate-solubilizing rhizobacteria, mycorrhizal fungi and rhizobium improve the agronomic efficiency of rock phosphate for legume crops. Nutr Cycl Agroecosyst 63:35–42CrossRefGoogle Scholar
  13. Barrow JR, Osuna P (2002) Phosphorus solubilization and uptake by dark septate fungi in fourwing saltbush, Atriplex canescens (Pursh) Nutt. J Arid Environ 51:449–459CrossRefGoogle Scholar
  14. Bethlenfalvay GJ, Brown MS, Pacovsky RS (1982) Relationships between host and endophyte development in mycorrhizal soybeans. New Phytol 90:537–543CrossRefGoogle Scholar
  15. Bethlenfalvay GJ, Phillips DA (1977) Photosynthetic efficiency and nitrogen fixation in Phaseolus vulgaris. In: Hollaender A (ed) Genetic engineering for nitrogen fixation. Plenum, New YorkGoogle Scholar
  16. Bever JD, Schultz PA, Pringle A, Morton JB (2001) Arbuscular mycorrhizal fungi: more diverse than meets the eye, and the ecological tale of why. Bioscience 51(11):923–932CrossRefGoogle Scholar
  17. Blanke V, Renker C, Wagner M, Füllner K, Held M, Kuhn AJ, Buscot F (2005) Nitrogen supply affects arbuscular mycorrhizal colonization of Artemisia vulgaris in phosphate-polluted field site. New Phytol 166:981–992PubMedCrossRefGoogle Scholar
  18. Breuillin F, Schramm J, Hajirezaei M, Ahkami A, Favre P, Druege U, Hause B, Bucher M, Kretzschmar T, Bossolini E, Kuhlemeier C, Martinoia E, Franken P, Scholz U, Reinhardt D (2010) Phosphate systemically inhibits development of arbuscular mycorrhiza in Petunia hybrida and represses genes involved in mycorrhizal functioning. Plant J 64(6):1002–1017PubMedCrossRefGoogle Scholar
  19. Buresh RJ, Smithson PC (1997) Building soil phosphorus capital in Africa. In: Buresh RJ, Sanchez PA, Calhoun F. (ed) Replenishing soil fertility in Africa. Soil Science Society of America, America Society of Agronomy, Madison, pp 111–150Google Scholar
  20. Cavagnaro TR (2008) The role of arbuscular mycorrhizas in improving plant zinc nutrition under low soil zinc concentrations: a review. Plant Soil 304:315–325CrossRefGoogle Scholar
  21. Douglas LA, Weaver RW (1993) Distribution of fixed-N and nitrate-N in cowpea during pod development. 12th International Plant Nutrition Colloquium, Perth, pp 353–354Google Scholar
  22. Gericke S, Kurmies B (1952) The colorimetric determination of phosphoric acid ammonium vanadate molybdate and its application in plant analysis. J Plant Nutr Soil Sci 159:11–21Google Scholar
  23. Giami S, Akosu M, Emelike J (2001) Evaluation of selected food attributes of four advanced lines of ungerminated and germinated Nigerian cowpea (Vigna unguiculata L. Walp). Plant Foods Human Nutr 56:61–73CrossRefGoogle Scholar
  24. Gueye M, Diemt HG, Dommergues YR (1987) Variation in N2 fixation, N and P contents of mycorrhizal Vigna unguiculata in relation to the progressive development of extraradical hyphae of Glomus mosseae. Mircen J 3:75–86CrossRefGoogle Scholar
  25. Gweyi-Onyango JP, Neumann G, Romheld V (2005) The role of nitrogen forms on solubilisation and utilization of rock phosphate by tomato plants. In: Tenywa JC, Adipala E, Nampala P, Tusiime G, Kyamuhangire W (ed). African Crop Science Conference Proceedings, KampalaGoogle Scholar
  26. Hasbullah MP, MCNeil A (2011) Legume residue influence arbuscular mycorrhizal colonisation and P uptake by wheat. Biol Fert Soils 47:701–707CrossRefGoogle Scholar
  27. Hawkins HJ, Johansen A, George E (2000) Uptake and transport of organic and inorganic nitrogen by arbuscular mycorrhizal fungi. Plant Soil 226:275–285CrossRefGoogle Scholar
  28. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. University of California, College of Agriculture, BerkleyGoogle Scholar
  29. Islam R, Ayanaba A, Sanders FE (1980) Response of cowpea (Vigna unguiculata) to inoculation with VA mycorrhizal fungi and to rock phosphate fertilization in some unsterile Nigerian soils. Plant Soi1 54:107–117CrossRefGoogle Scholar
  30. Jemo M, Abaidoo RC, Nolte C, Horst WJ (2006) Genotypic variation for phosphorous uptake and dinitrogen fixation in cowpea on low-phosphorus soils of southern Cameroon. J Plant Nutr Soil Sci 169:816–818CrossRefGoogle Scholar
  31. Jemo M, Nolte C, Nwaga D (2007) Biomass production, N and P uptake of Mucuna after Bradyrhizobia and arbuscular mycorrhyzal fungi inoculation, and P application on acid soil of Southern Cameroon. In: Bationo A (ed) Advances in integrated soil fertility management in Sub-Saharan Africa: challenges and opportunities. Springer, Dordrecht, pp 855–864CrossRefGoogle Scholar
  32. Jemo M, Nolte C, Tchienkoua M, Abaidoo RC (2010) Biological nitrogen fixation potential by soybeans in two low-P soils of southern Cameroon. Nutr Cycl Agroecosyst 88:49–58CrossRefGoogle Scholar
  33. Johansen A (1999) Depletion of soil mineral N by roots of Cucumis sativus L. colonized or not by arbuscular mycorrhizal fungi. Plant Soil 209:119–127CrossRefGoogle Scholar
  34. Koide RT (1991) Nutrient supply, nutrient demand and plant response to mycorrhizal infection. New Phytol 117:365–386CrossRefGoogle Scholar
  35. Kormanick P, McGraw AC (1982) Quantification of vesicular-arbuscular mycorrhizae in plant roots. In: Schenck NC (ed) Methods and principles of mycorrhizal research. The American Phytopathological Society, St Paul, Minnesota, pp 37–45Google Scholar
  36. Lekberg Y, Koide RT (2005) Arbuscular mycorrhizal fungi, rhizobia, available soil P and nodulation of groundnut (Arachis hypogaea) in Zimbabwe. Agr Ecosyst Environ 110:143–148CrossRefGoogle Scholar
  37. Mark BP, John SP, Craig AA (1983) Mobilization of nitrogen in fruiting plants of a cultivar of cowpea. J Exp Bot 34(5):563–578CrossRefGoogle Scholar
  38. Marschner H, Dell B (1994) Nutrient uptake in mycorrhizal symbiosis. Plant Soil 159:89–102Google Scholar
  39. Martins LMV, Xavier GR, Rangel FW, Ribeiro JRA, Neves MCP, Morgado LB, Rumjalek NG (2003) Contribution of biological nitrogen fixation to cowpea: a strategy for improving grain yields in the semi-arid regions of Brasil. Biol Fertil Soils 38:333–339CrossRefGoogle Scholar
  40. Mortimer PE, Pérez-Fernández MA, Valentine AJ (2008) The role of arbuscular mycorrhizal colonization in the carbon and nutrient economy of the tripartite symbiosis with nodulated Phaseolus vulgaris. Soil Biol Biochem 40:1019–1027CrossRefGoogle Scholar
  41. Ngwene B, Gabriel E, Eckhard G (2013) Influence of different mineral nitrogen sources (NO3 -N vs. NH4 +-N) on arbuscular mycorrhiza development and N transfer in a Glomus intraradices–cowpea symbiosis. Mycorrhiza 23:107–117PubMedCentralPubMedCrossRefGoogle Scholar
  42. Ngwene B, George E, Claussen W, Neumann E (2010) Phosphorus uptake by cowpea plants from sparingly available or soluble sources as affected by N-form and arbuscular–mycorrhiza–fungal inoculation. J Plant Nutr Soil Sci 173:353–359CrossRefGoogle Scholar
  43. Rajapakse S, Zuberer DA, Miller JC Jr (1989) Influence of phosphorus level on VA mycorrhizal colonization and growth of cowpea cultivars. Plant Soil 114:45–52CrossRefGoogle Scholar
  44. Shehu HE, Kwari JD, Sandabe MK (2010) Effects of N, P, K fertilizers on yield, content and uptake of N, P and K by sesame. Int J Agric Biol 12:845–850Google Scholar
  45. Smith SE, Jakobsen I, Gronlund M, Smith FA (2011) Roles of arbuscular mycorrhizas in plant phosphorus nutrition: interactions between pathways of phosphorus uptake in arbuscular mycorrhizal roots have important implications for understanding and manipulating plant phosphorus acquisition. Plant Physiol 156:1050–1057PubMedCentralPubMedCrossRefGoogle Scholar
  46. Smith SE, Read DJ (1997) Mycorrhizal symbiosis. Academic Press, LondonGoogle Scholar
  47. Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic, CambridgeGoogle Scholar
  48. Smith SE, Smith FA, Jakobsen I (2003) Mycorrhizal fungi can dominate phosphate supply to plants irrespective of growth responses. Plant Physiol 133:16–20PubMedCentralPubMedCrossRefGoogle Scholar
  49. Taffouo VD, Meguekam L, Amougou A, Ourry A (2010) Effects of germination, plant growth and accumulation of metabolite in five leguminous plants. J Agr Sci Technol 4(2):27–33Google Scholar
  50. Tanaka Y, Yano K (2005) Nitrogen delivery to maize via mycorrhizal hyphae depends on the form of N supplied. Plant cell and Environ 28:1247–1254CrossRefGoogle Scholar
  51. Tobar R, Azcόn R, Barea (1994) Improved nitrogen uptake and transport from 15 N-labelled nitrate by external hyphae of arbuscular mycorrhiza under water-stressed conditions. New Phytol 126:119–122CrossRefGoogle Scholar
  52. Vierheilig H, Coughlan AP, Wyss U, Piche Y (1998) Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Appl Environ Microbiol 63:5004–5007Google Scholar
  53. Voisin AS, Salon C, Munier-Jolain NG, Ney B (2002) Effect of mineral nitrogen on nitrogen nutrition and biomass partitioning between the shoot and roots of pea (Pisum sativum L.). Plant Soil 242:251–262CrossRefGoogle Scholar
  54. Watts-Williams SJ, Cavagnaro TR (2012) Arbuscular mycorrhizas modify tomato responses to zinc and phosphorus addition. Biol Fertil Soils 48:285–294CrossRefGoogle Scholar
  55. Westermann DT, Porter LK, O’Deen (1985) Nitrogen partitioning and mobilization patterns in bean plants. Crop Sci 25:225–229CrossRefGoogle Scholar
  56. Wu XQ, Hou LL, Sheng JM, Ren JH, Zheng L, Chen D, Ye JR (2012) Effects of ectomycorrhizal fungus Boletus edulis and mycorrhiza helper Bacillus cereus on the growth and nutrient uptake by Pinus thunbergii. Biol Fertil Soils 48(4):385–391CrossRefGoogle Scholar
  57. Yost RS, Fox DRL (1979) Contribution of mycorrhizae to P nutrition of crops growing on an oxisol. Agron J 71:903–908CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Victor Désiré Taffouo
    • 1
    Email author
  • Benard Ngwene
    • 3
  • Amougou Akoa
    • 2
  • Philipp Franken
    • 3
  1. 1.Department of Botany, Faculty of ScienceUniversity of DoualaDoualaCameroon
  2. 2.Department of Biology and Plant PhysiologyUniversity of Yaounde IYaoundeCameroon
  3. 3.Department of Plant NutritionLeibniz-Institute of Vegetable and Ornamental Crops Grossbeeren/Erfurt e.V.GrossbeerenGermany

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