, Volume 23, Issue 5, pp 349–358 | Cite as

Assessment of ectomycorrhizal fungal communities in the natural habitats of Tuber magnatum (Ascomycota, Pezizales)

  • M. Leonardi
  • M. Iotti
  • M. Oddis
  • G. Lalli
  • G. PacioniEmail author
  • P. Leonardi
  • S. Maccherini
  • C. Perini
  • E. Salerni
  • A. Zambonelli
Original Paper


The ectomycorrhizal (ECM) fungal communities of four natural Tuber magnatum truffle grounds, located in different Italian regions (Abruzzo, Emilia-Romagna, Molise, and Tuscany), were studied. The main objective of this study was to characterize and compare the ECM fungal communities in the different regions and in productive (where T. magnatum ascomata were found) and nonproductive points. More than 8,000 (8,100) colonized root tips were counted in 73 soil cores, and 129 operational taxonomic units were identified using morphological and molecular methods. Although the composition of the ECM fungal communities studied varied, we were able to highlight some common characteristics. The most plentiful ECM fungal taxa belong to the Thelephoraceae and Sebacinaceae families followed by Inocybaceae and Russulaceae. Although several ectomycorrhizas belonging to Tuber genus were identified, no T. magnatum ectomycorrhizas were found. The putative ecological significance of some species is discussed.


White truffle Ecology Biodiversity Statistics 



This work was financially supported by the regions Tuscany, Emilia-Romagna, Abruzzo, and Molise (project MAGNATUM). We are grateful to Juliet Macan, a language technology consultant, for the improvement of the English manuscript.

Supplementary material

572_2012_474_Fig2_ESM.jpg (38 kb)
Fig. S1

Location of the four areas: 1 Emilia-Romagna, 2 Tuscany, 3 Abruzzo, and 4 Molise (JPEG 37 kb)

572_2012_474_MOESM1_ESM.xls (50 kb)
Table S1 Recognized OTUs in total frequency order, average abundance in all soil cores, frequency of the presence in nonproductive and productive points, GenBank accessions, e-myco code; ns not submitted; short sequence (only ITS1 or ITS2); nsm no significant matches (XLS 50 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • M. Leonardi
    • 1
  • M. Iotti
    • 3
  • M. Oddis
    • 1
  • G. Lalli
    • 1
  • G. Pacioni
    • 1
    Email author
  • P. Leonardi
    • 2
  • S. Maccherini
    • 2
  • C. Perini
    • 2
  • E. Salerni
    • 2
  • A. Zambonelli
    • 3
  1. 1.Department of Life, Health and Environmental SciencesUniversity of L’AquilaL’AquilaItaly
  2. 2.Biodiversity and Conservation Network (BIOCONNET), Department of Environmental Science “G. Sarfatti”University of SienaSienaItaly
  3. 3.Dipartimento di Scienze AgrarieAlma Mater Studiorum University of BolognaBolognaItaly

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