Mechanical soil disturbance as a determinant of arbuscular mycorrhizal fungal communities in semi-natural grassland
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While the effect of disturbance on overall abundance and community composition of arbuscular mycorrhizal (AM) fungi has been researched in agricultural fields, less is known about the impact in semi-natural grasslands. We sampled two AM plant species, Festuca brevipila and Plantago lanceolata, from an ongoing grassland restoration experiment that contained replicated plowed and control plots. The AM fungal community in roots was determined using nested PCR and LSU rDNA primers. We identified 38 phylotypes within the Glomeromycota, of which 29 belonged to Glomus A, six to Glomus B, and three to Diversisporaceae. Only three phylotypes were closely related to known morphospecies. Soil disturbance significantly reduced phylotype richness and changed the AM fungal community composition. Most phylotypes, even closely related ones, showed little or no overlap in their distribution and occurred in either the control or disturbed plots. We found no evidence of host preference in this system, except for one phylotype that preferentially seemed to colonize Festuca. Our results show that disturbance imposed a stronger structuring force for AM fungal communities than did host plants in this semi-natural grassland.
KeywordsLSU rDNA Plowing Calcareous grassland Phylogenetic networks
This research has been made possible by funding from The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS). Ylva Lekberg is grateful for funding from the Marie Curie. We would also like to thank two anonymous reviewers for important comments on the manuscript.
- Andersson O (1950) The Scanian sand vegetation—a survey. Botaniska Notiser 2:145–172Google Scholar
- Brundrett M, Bougher N, Dell B, Grove T, Malajczuk N (1996) Working with mycorrhizas in forestry and agriculture. ACIAR, Canberra, AustraliaGoogle Scholar
- Colwell RK (2009) EstimateS: statistical estimation of species richness and shared species from samples. Version 8.2. User’s guide and application published at: http://purl.oclc.org/estimates. Accessed 5th December 2009
- Kabir Z (2005) Tillage or no-tillage: impact on mycorrhizae. Can J Plant Sci 85:23–29Google Scholar
- R Developement Core Team (2008) R: A language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
- Smith S, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Elsevier, Great BritainGoogle Scholar
- Violi HA, Barrientos-Priego AF, Wright SF, Escamilla-Prado E, Morton JB, Menge JA, Lovatt CJ (2008) Disturbance changes arbuscular mycorrhizal fungal phenology and soil glomalin concentrations but not fungal spore composition in montane rainforests in Veracruz and Chiapas, Mexico. Forest Ecol Manag 254:276–290CrossRefGoogle Scholar