Mycorrhiza

, Volume 21, Issue 1, pp 17–25

The AD-type ectomycorrhizas, one of the most common morphotypes present in truffle fields, result from fungi belonging to the Trichophaea woolhopeia species complex

  • Andrea Rubini
  • Beatrice Belfiori
  • Valentina Passeri
  • Leonardo Baciarelli Falini
  • Sergio Arcioni
  • Claudia Riccioni
  • Francesco Paolocci
Original Paper

Abstract

Belowground ectomycorrhizal communities are often species rich. Characterization of the ectomycorrhizas (ECMs) underneath native truffle areas and/or cultivation sites is particularly relevant to identifying fungal species that might interfere with or promote truffle propagation and fruiting. Fungal identification at the genus/species level can now be achieved by combining detailed morphological and anatomical descriptions with molecular approaches. In a survey of the mycorrhizal biodiversity of Tuber melanosporum orchards and inoculated host plants in nurseries, we repeatedly sampled ECMs with morphological features resembling those of the ECMs widely known as the AD type. Despite the fact that the AD type is regarded as one of the most competitive fungal species towards Tuber spp., its taxonomical rank has yet to be resolved. By analyzing the 28S and internal transcribed spacer (ITS) rDNA regions, here, we show that AD-type ECMs result from host plant colonization by the pyronemataceous species Trichophaea woolhopeia. Further to this, the 28S and ITS phylogenetic trees built from the AD-type ECMs analyzed sustain the hypothesis that T. woolhopeia is a species complex.

Keywords

Ectomycorrhiza Tuber Molecular identification LSU ITS Trichophaea woolhopeia 

Supplementary material

572_2010_308_MOESM1_ESM.doc (62 kb)
Fig. S1Multiple sequence alignment of the large subunit region. (DOC 61 kb)
572_2010_308_MOESM2_ESM.doc (62 kb)
Fig. S2Multiple sequence alignment of the internal transcribed spacer region. (DOC 61 kb)
572_2010_308_MOESM3_ESM.doc (240 kb)
Fig. S3Neighbor-joining tree based on large subunit sequences from AD-type samples and other Pyronemataceae. Numbers near the branches represent the bootstrap values (1,000 replicates). The sequences from AD-type samples obtained in this study are given in bold. (DOC 240 kb)
572_2010_308_MOESM4_ESM.doc (242 kb)
Fig. S4Maximum-likelihood tree based on large subunit sequences from AD-type samples and other Pyronemataceae. Numbers near the branches represent the bootstrap values (1,000 replicates). The sequences from AD-type samples obtained in this study are given in bold. (DOC 241 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Andrea Rubini
    • 1
  • Beatrice Belfiori
    • 1
  • Valentina Passeri
    • 1
  • Leonardo Baciarelli Falini
    • 2
  • Sergio Arcioni
    • 1
  • Claudia Riccioni
    • 1
  • Francesco Paolocci
    • 1
  1. 1.National Research Council, Plant Genetics Institute, Perugia DivisionPerugiaItaly
  2. 2.Department of Applied BiologyUniversity of PerugiaPerugiaItaly

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