Advertisement

Mycorrhiza

, Volume 20, Issue 6, pp 399–406 | Cite as

Nickel remediation by AM-colonized sunflower

  • Keomany Ker
  • Christiane CharestEmail author
Original Paper

Abstract

This greenhouse study aimed to examine the contribution of arbuscular mycorrhizal (AM) colonization on the uptake of and tolerance to nickel (Ni) in sunflower (Helianthus annuus L.). We hypothesized that AM colonization increases Ni content and tolerance in sunflower grown under varying soil Ni concentrations. The combined effect of AM colonization and soil Ni input on the assimilation of nitrogen, in particular the activity of glutamine synthetase (GS), in sunflower plants was also investigated. A factorial experimental design was performed with sunflower cv. Lemon Queen, with or without the AM fungus, Glomus intraradices Schenck & Smith, and treated with 0, 100, 200, or 400 mg Ni kg−1 dry soil (DS). The AM colonization significantly enhanced plant growth and Ni content, especially at the lower soil Ni treatments. Furthermore, the AM plants exposed to the highest soil Ni level of 400 mg Ni kg−1 DS had a significantly higher shoot Ni extracted percentage than non-AM plants, suggesting that the AM symbiosis contributed to Ni uptake, then its translocation from roots to shoots. The AM colonization also significantly increased the GS activity in roots, this being likely an indicator of an enhanced Ni tolerance. These findings support the hypothesis that AM symbiosis contributes to an enhanced Ni plant uptake and tolerance and should be considered as part of phytoremediation strategies.

Keywords

Arbuscular mycorrhiza Glomus intraradices Helianthus annuus Metal Phytoremediation 

Notes

Acknowledgment

This work was funded by a grant of the Natural Science and Engineering Research Council of Canada to C. Charest.

References

  1. Ahonen-Jonnarth U, Finlay RD (2001) Effects of elevated nickel and cadmium concentrations on growth and nutrient uptake of mycorrhizal and non-mycorrhizal Pinus sylvestris seedlings. Plant Soil 236:129–138CrossRefGoogle Scholar
  2. Audet P, Charest C (2006) Effects of AM colonization on ‘wild tobacco’ plants grown in zinc-contaminated soil. Mycorrhiza 16:277–283CrossRefPubMedGoogle Scholar
  3. Audet P, Charest C (2007) Heavy metal phytoremediation from a meta-analytical perspective. Environ Pollut 147:231–237CrossRefPubMedGoogle Scholar
  4. Audet P, Charest C (2008) Allocation plasticity and plant–metal partitioning: metal-analytical perspectives in phytoremediation. Environ Pollut 156:290–296CrossRefPubMedGoogle Scholar
  5. Baker AJM (1981) Accumulators and excluders—strategies in the response of plants to heavy metals. J Plant Nutr 3:643–654CrossRefGoogle Scholar
  6. Bhatia NP, Walsh KB, Baker AJM (2005) Detection and quantification of ligands involved in nickel detoxification in a herbaceous Ni hyperaccumulator Stackhousia tryonii Bailey. J Exper Bot 56:1343–1349CrossRefGoogle Scholar
  7. Chen B, Christie P, Li X (2001) A modified glass bead compartment cultivation system for studies on nutrient and trace metal uptake by arbuscular mycorrhiza. Chemosphere 42:185–192CrossRefPubMedGoogle Scholar
  8. Cunningham SD, Berti WR, Huang JW (1995) Phytoremediation of contaminated soils. Trends Biotech 13:393–397CrossRefGoogle Scholar
  9. Dalpé Y (1993) Vesicular–arbuscular mycorrhizae. In: Carter MR (ed) Soil sampling and methods of analysis. CRC, Boca Raton, pp 287–301Google Scholar
  10. Davies FT, Puryear JD, Newton RJ, Egilla JN, Saraiva Grossi JA (2002) Mycorrhizal fungi increase chromium uptake by sunflower plants: influence on tissue mineral concentration, growth, and gas exchange. J Plant Nutr 25:2389–2407CrossRefGoogle Scholar
  11. Gildon A, Tinker PB (1983) Interactions of vesicular–arbuscular mycorrhizal infection and heavy metals in plants: the effects of heavy metals on the development of vesicular–arbuscular mycorrhizae. New Phytol 95:147–161CrossRefGoogle Scholar
  12. Hewitt EJ, Smith TA (1975) Plant mineral nutrition. Wiley, New York, pp 32–33Google Scholar
  13. Insightful Corp. (2003) S-Plus 6.2 for Windows. Insightful Corp., SeattleGoogle Scholar
  14. Joner EJ, Briones R, Leyval C (2000) Metal-binding capacity of arbuscular mycorrhizal mycelium. Plant Soil 226:227–234CrossRefGoogle Scholar
  15. Jones MD, Hutchinson TC (1988) Nickel toxicity in mycorrhizal birch seedlings infected with Lactarius rufus or Scleroderma flavidum. Uptake of nickel, calcium, magnesium, phosphorus and iron. New Phytol 108:461–470CrossRefGoogle Scholar
  16. Kastori P, Petrovic N, Petrovic M (1996) Effects of lead on water relations, proline concentration and nitrate reductase activity in sunflower plants. Acta Agron Hungar 44:21–28Google Scholar
  17. Killham K, Firestone MK (1983) Vesicular arbuscular mycorrhizal mediation of grass response to acidic and heavy metal depositions. Plant Soil 72:39–48CrossRefGoogle Scholar
  18. Krämer U, Smith RD, Wenzel WW, Raskin I, Salt DE (1997) The role of metal transport and tolerance in nickel hyperaccumulation by Thlaspi goesingense Hálácsy. Plant Physiol 115:1641–1650PubMedGoogle Scholar
  19. Krämer U, Pickering IJ, Prince RC, Raskin I, Salt DE (2000) Subcellular localization and speciation of nickel in hyperaccumulator and non-hyperaccumulator Thlaspi species. Plant Physiol 122:1343–1353CrossRefPubMedGoogle Scholar
  20. Leopold I, Günther D, Schmidt J, Neumann D (1999) Phytochelatins and heavy metal tolerance. Phytochemistry 50:1323–1328CrossRefGoogle Scholar
  21. Leyval C, Turnau K, Haselwandter K (1997) Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects. Mycorrhiza 7:139–153CrossRefGoogle Scholar
  22. Li YM, Chaney R, Brewer E, Roseberg R, Angle JS, Baker A, Reeves R, Nelkin J (2003) Development of a technology for commercial phytoextraction of nickel: economic and technical considerations. Plant Soil 249:107–115CrossRefGoogle Scholar
  23. Liu A, Hamel C, Hamilton RI, Ma BL, Smith DL (2000) Acquisition of Cu, Zn, Mn and Fe by mycorrhizal maize (Zea mays L.) grown in soil at different P and micronutrient levels. Mycorrhiza 9:331–336CrossRefGoogle Scholar
  24. Marschner H (1995) Mineral nutrition in higher plants, 2nd edn. Academic, LondonGoogle Scholar
  25. McGrath SP, Zhao FJ, Lombi E (2001) Plant and rhizosphere processes involved in phytoremediation of metal-contaminated soils. Plant Soil 232:207–214CrossRefGoogle Scholar
  26. Reeves RD, Baker AJM (2000) Metal-accumulating plants. In: Raskin I, Ensley BD (eds) Phytoremediation of toxic metals: using plants to clean up the environment. Wiley, New York, pp 193–229Google Scholar
  27. Reeves RD, Macfarlane RM, Brooks RR (1983) Accumulation of nickel and zinc by western North American genera containing serpentine-tolerant species. Amer J Bot 70:1297–1303CrossRefGoogle Scholar
  28. Saber NE, Abdel-Moneim AM, Barakat SY (1999) Role of organic acids in sunflower tolerance to heavy metals. Biol Planta 42:65–73CrossRefGoogle Scholar
  29. Sagner S, Kneer R, Wanner G, Cosson JP, Deus-Neumann B, Zenk MH (1998) Hyperaccumulation, complexation and distribution of nickel in Sebertia acuminata. Phytochemistry 47:339–347CrossRefPubMedGoogle Scholar
  30. Slivinskaya RB (1991) Nickel effect on sunflower leaf cell membranes. Acta Bot Neerl 40:133–138Google Scholar
  31. Toussaint JP, St-Arnaud M, Charest C (2004) Nitrogen transfer and assimilation between the arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith and Ri T-DNA roots of Daucus carota L in an in vitro compartmented system. Can J Microbiol 50:251–260CrossRefPubMedGoogle Scholar
  32. Turgut C, Pepe MK, Cutright TJ (2004) The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus. Environ Pollut 131:147–154CrossRefPubMedGoogle Scholar
  33. Turnau K, Mesjasz-Przybylowicz J (2003) Arbuscular mycorrhiza of Berkheya coddii and other Ni-hyperaccumulating members of Asteraceae from ultramafic soils in South Africa. Mycorrhiza 13:185–190CrossRefPubMedGoogle Scholar
  34. Ximénez-Embún P, Alonso I, Madrid-Albarrán Y, Cámara C (2004) Establishment of selenium uptake and species distribution in lupine, Indian mustard, and sunflower plants. J Agric Food Chem 52:832–838CrossRefPubMedGoogle Scholar
  35. Yang XH, Brooks RR, Jaffré T, Lee J (1985) Elemental levels and relationships in the Flacourtiaceae of New Caledonia and their significance for the evaluation of the ‘serpentine problem’. Plant Soil 87:281–291CrossRefGoogle Scholar
  36. Zornoza P, Robles S, Martin N (1999) Alleviation of nickel toxicity by ammonium supply to sunflower. Plant Soil 208:221–226CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Department of BiologyUniversity of OttawaOttawaCanada

Personalised recommendations