Mycorrhiza

, Volume 18, Issue 8, pp 443–449

Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)

  • Beatriz Águeda
  • Javier Parladé
  • Luz Marina Fernández-Toirán
  • Óscar Cisneros
  • Ana María de Miguel
  • María Pilar Modrego
  • Fernando Martínez-Peña
  • Joan Pera
Short Note

Abstract

Ectomycorrhizas of Boletus aereus, Boletus edulis, and Boletus reticulatus were synthesized with Cistus sp. under laboratory conditions using synthesis tubes filled with a mixture of sterilized peat-vermiculite and nutrient solution. The fungal strains isolated from sporocarps were identified by molecular techniques. The inoculated seedlings were grown for 4–5 months. The ectomycorrhizas formed were described based on standard morphological and anatomical characters. The three ectomycorrhizas described were very similar, with white monopodial-pinnate morphology, a three-layered plectenchymatous mantle on plan view and boletoid rhizomorphs.

Keywords

Pure culture synthesis Ectomycorrhizas Boletus Anatomy Morphology Description ITS rDNA 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Beatriz Águeda
    • 1
  • Javier Parladé
    • 2
  • Luz Marina Fernández-Toirán
    • 1
    • 3
  • Óscar Cisneros
    • 1
  • Ana María de Miguel
    • 4
  • María Pilar Modrego
    • 1
  • Fernando Martínez-Peña
    • 1
  • Joan Pera
    • 2
  1. 1.Departamento de Investigación y Experiencias Forestales de ValonsaderoConsejería de Medio AmbienteSoriaSpain
  2. 2.Centre de CabrilsCabrilsSpain
  3. 3.Escuela Universitaria de Ingenierías AgrariasUniversidad de ValladolidSoriaSpain
  4. 4.Departamento de Biología VegetalUniversidad de Navarra, Facultad de Ciencias, Sección BotánicaPamplonaSpain

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