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Mycorrhiza

, Volume 18, Issue 6–7, pp 339–354 | Cite as

Diversity and structure of ectomycorrhizal and co-associated fungal communities in a serpentine soil

  • Alexander UrbanEmail author
  • Markus Puschenreiter
  • Joseph Strauss
  • Markus Gorfer
Original Paper

Abstract

The community of ectomycorrhizal (ECM) and co-associated fungi from a serpentine site forested with Pinus sylvestris and Quercus petraea was explored, to improve the understanding of ECM diversity in naturally metalliferous soils. ECM fungi were identified by a combination of morphotyping and direct sequencing of the nuclear ribosomal internal transcribed spacer region 2 and of a part of the large-subunit region. Co-associated fungi from selected ECM were identified by restriction fragment length polymorphism and sequencing of representative clones from libraries. Polymerase chain reaction with species-specific primers was applied to assess patterns of association of ECM and co-associated fungi. Twenty ECM species were differentiated. Aphyllophoralean fungi representing several basidiomycete orders and Russulaceae were dominant. Phialocephala fortinii was the most frequently encountered taxon from the diverse assemblage of ECM co-associated fungi. A ribotype representing a deeply branching ascomycete lineage known from ribosomal deoxyribonucleic acid sequences only was detected in some ECM samples. A broad taxonomic range of fungi have the potential to successfully colonise tree roots under the extreme edaphic conditions of serpentine soils. Distribution patterns of ECM-co-associated fungi hint at the importance of specific inter-fungal interactions, which are hypothesised to be a relevant factor for the maintenance of ECM diversity.

Keywords

Pinus sylvestris Quercus petraea Ectomycorrhiza ECM-co-associated fungi Phialocephala fortinii Serpentine Rhizosphere Diversity Heavy metal toxicity 

Notes

Acknowledgements

This work was supported by grant P15357 from the Austrian Science Foundation (FWF) and grant LS149 from the Vienna Science and Technology Fund (WWTF) to JS. The authors thank Anton Russell and two anonymous reviewers for critical reading of the manuscript and helpful suggestions.

Supplementary material

572_2008_189_MOESM1_ESM.doc (143 kb)
Table S1 Comparison of ITS sequences from P. fortinii CSP. P. fortinii ITS sequences from Redlschlag and from Zürichberg (Grünig et al. 2004) were aligned with the ClustalW algorithm. Only positions with differences are shown with discriminating nucleotides highlighted in bold. Numbering of positions is according to sequence AY347406 (CSP3, strain 144-4). Classification into CSP is according to Grünig et al. (2004). Missing characters indicate missing sequence information, and ‘–’ indicates a gap. The poly-T stretch with variable length at the end of the ITS2-region (starting at position 500 in AY347406) is not included in the comparison. #: number of sequences with 100% identity from the Zürichberg sequence set (Grünig et al. 2004) (DOC 143 KB)
572_2008_189_MOESM2_ESM.pdf (9 kb)
Fig. S1 Phylogenetic tree for RSEM01_13 based on partial 18S rDNA sequences. A multiple alignment of a set of published partial 18S rDNA sequences from all recognised groups of fungi including a set of 18S rDNA sequences from AG1 was generated with the ClustalW algorithm in ARB. For construction of the phylogenetic tree, the Phylip package in ARB was used with a filter for positions covered by sequences from the unknown ascomycete lineage. Entomophaga conglomerata was used as the out-group for routing. Branches were collapsed for phyla, except for ascomycota, where branches were collapsed for sub-phyla (shaded triangles). The order Mucorales was separated from the remaining zygomycota. The phyla of chytridiomycota and zygomycota could not be separated based on partial 18S rDNA sequences. Separation of a group of hitherto uncultivated fungi—Ascomycota Group I (darkly shaded triangle)—including the enigmatic ascomycete RSEM01_13 was supported by different algorithms used for construction of the tree (neighbour joining, Bayesian analysis). Numbers inside or next to triangles indicate number of species included in the collapsed branch (PDF 9.23 KB)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Alexander Urban
    • 1
    • 2
    • 3
    Email author
  • Markus Puschenreiter
    • 4
  • Joseph Strauss
    • 1
    • 2
  • Markus Gorfer
    • 1
    • 2
  1. 1.Fungal Genomics UnitAustrian Research CentersViennaAustria
  2. 2.Institute of Applied Genetics and Cell BiologyUniversity of Natural Resources and Applied Life SciencesViennaAustria
  3. 3.Department for Systematic and Evolutionary Botany, Mycology Research GroupUniversity of ViennaViennaAustria
  4. 4.Department of Forest and Soil SciencesUniversity of Natural Resources and Applied Life SciencesViennaAustria

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