Mycorrhiza

, Volume 17, Issue 1, pp 37–49 | Cite as

Cooccurring plants forming distinct arbuscular mycorrhizal morphologies harbor similar AM fungal species

  • Evelyn Matekwor Ahulu
  • Armelle Gollotte
  • Vivienne Gianinazzi-Pearson
  • Masanori Nonaka
Original Paper

Abstract

Arbuscular mycorrhizal (AM) fungal spores were isolated from field transplants and rhizosphere soil of Hedera rhombea (Miq) Bean and Rubus parvifolius L., which form Paris-type and Arum-type AM, respectively. DNA from the spore isolates was used to generate molecular markers based on partial large subunit (LSU) ribosomal RNA (rDNA) sequences to determine AM fungi colonizing field-collected roots of the two plant species. Species that were isolated as spores and identified morphologically and molecularly were Gigaspora rosea and Scutellospora erythropa from H. rhombea, Acaulospora longula and Glomus etunicatum from R. parvifolius, and Glomus claroideum from both plants. The composition of the AM fungal communities with respect to plant trap cultures was highly divergent between plant species. Analysis of partial LSU rDNA sequences amplified from field-collected roots of the two plant species with PCR primers designed for the AM fungi indicated that both plants were colonized by G. claroideum, G. etunicatum, A. longula, and S. erythropa. G. rosea was not detected in the field-collected roots of either plant species. Four other AM fungal genotypes, which were not isolated as spores in trap cultures from the two plant species, were also found in the roots of both plant species; two were closely related to Glomus intraradices and Glomus clarum. One genotype, which was most closely related to Glomus microaggregatum, was confined to R. parvifolius, whereas an uncultured Glomeromycotan fungus occurred only in roots of H. rhombea. S. erythropa was the most dominant fungus found in the roots of H. rhombea. The detection of the same AM fungal species in field-collected roots of H. rhombea and R. parvifolius, which form Paris- and Arum-type AM, respectively, shows that AM morphology in these plants is strongly influenced by the host plant genotypes as appears to be the case in many plant species in natural ecosystems, although there are preferential associations between the hosts and colonizing AM fungi in this study.

Keywords

Arum-/Paris-type mycorrhiza Sand dune AM fungal genotype LSU rDNA Nested PCR 

Notes

Acknowledgments

This work was partly supported by the International Tropical Timber Organization (ITTO, travel grant to EMA). The authors are grateful to Diederik van Tuinen (INRA) for the helpful discussion and advice, to Odile Chatagnier and Valerie Monfort (INRA) for their technical assistance, and to Joe Morton (INVAM) for providing the culture of S. erythropa.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Evelyn Matekwor Ahulu
    • 1
    • 2
  • Armelle Gollotte
    • 3
  • Vivienne Gianinazzi-Pearson
    • 3
  • Masanori Nonaka
    • 2
  1. 1.Forestry Research Institute of GhanaUST, KumasiGhana
  2. 2.Soil Science Laboratory, Faculty of AgricultureNiigata UniversityNiigataJapan
  3. 3.UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-Environnement, INRA-CMSEDijon CedexFrance

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