, Volume 16, Issue 3, pp 213–217 | Cite as

Limited transfer of nitrogen between wood decomposing and ectomycorrhizal mycelia when studied in the field

  • Håkan WallanderEmail author
  • Björn D. Lindahl
  • Lars Ola Nilsson
Short Note


Transfer of 15N between interacting mycelia of a wood-decomposing fungus (Hypholoma fasciculare) and an ectomycorrhizal fungus (Tomentellopsis submollis) was studied in a mature beech (Fagus sylvatica) forest. The amount of 15N transferred from the wood decomposer to the ectomycorrhizal fungus was compared to the amount of 15N released from the wood-decomposing mycelia into the soil solution as 15N-NH4. The study was performed in peat-filled plastic containers placed in forest soil in the field. The wood-decomposing mycelium was growing from an inoculated wood piece and the ectomycorrhizal mycelium from an introduced root from a mature tree. The containers were harvested after 41 weeks when physical contact between the two foraging mycelia was established. At harvest, 15N content was analyzed in the peat (total N and 15NH4 +) and in the mycorrhizal roots. A limited amount of 15N was transferred to the ectomycorrhizal fungus and this transfer could be explained by 15NH4 + released from the wood-decomposing fungus without involving any antagonistic interactions between the two mycelia. Using our approach, it was possible to study nutritional interactions between basidiomycete mycelia under field conditions and this and earlier studies suggest that the outcomes of such interactions are highly species-specific and depend on environmental conditions such as resource availability.


Antagonistic interactions Hypholoma Nitrogen transfer Mycorrhiza 15Tomentellopsis 



Funding was provided by the Royal Swedish Academy of Agriculture and Forestry (grants to H. Wallander). Thanks to Nancy Johnson for critical comments on the manuscript. Thanks to Stefan Olsson and von Reiserska Stiftelsen for giving us access to their forest and to Bo and Ingrid Wallander for letting us use Rye cottage as our field laboratory in the study.


  1. Abuzinadah RA, Read DJ (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants I. Utilization of peptides and proteins by ectomycorrhizal fungi. New Phytol 103:481–493CrossRefGoogle Scholar
  2. Agerer R (1987–1998) Color atlas of ectomycorrhizae, 1st–11th edn. Einhorn, Schwäbish GmündGoogle Scholar
  3. Baar J, Stanton NL (2000) Ectomycorrhizal fungi challenged by saprotrophic basidiomycetes and soil microfungi under different ammonium regimes in vitro. Mycol Res 104:691–697CrossRefGoogle Scholar
  4. Bending GD, Read DJ (1995) The structure and function of the vegetative mycelium of ectomycorrhizal plants. 6. Activities of nutrient mobilizing enzymes in birch litter colonized by Paxillus involutus (Fr) Fr. New Phytol 130:411–417CrossRefGoogle Scholar
  5. Boddy L, Watkinsson SC (1995) Wood decomposition, higher fungi, and their role in nutrient redistribution. Can J Bot 73(Suppl 1):1377–1383 (sect. E–H)CrossRefGoogle Scholar
  6. Boyle D (1998) Nutritional factors limiting the growth of Lentinula edodes and other white-rot fungi in wood. Soil Biol Biochem 30:817–823CrossRefGoogle Scholar
  7. Finlay RD, Ek H, Odham, G, Söderström B (1988) Mycelial uptake, translocation and assimilation of nitrogen from 15N-labelled ammonium by Pinus sylvestris plants infected with four different ectomycorrhizal fungi. New Phytol 110:59–66CrossRefGoogle Scholar
  8. Finlay RD, Ek H, Odham, Söderström B (1989) Uptake, translocation and assimilation of nitrogen from 15N-labelled ammonium and nitrate by intact ectomycorrhizal systems of Fagus sylvatica infected with Paxillus involutus. New Phytol 113:47–55CrossRefGoogle Scholar
  9. Holmer L, Stenlid J (1997) Competitive hierarchies of wood decomposing basidiomycetes in artificial systems based on variable inoculum sizes. Oikos 79:77–84CrossRefGoogle Scholar
  10. Koljalg U, Tammi H, Timmonen S, Agerer R, Sen R (2002) ITS rDNA sequence-based phylogenetic analysis of Tomentellopsis species from boreal and temperate forests, and the identification of pink-type ectomycorrhizas. Mycol Progr 1:81–92CrossRefGoogle Scholar
  11. Leake JR, Donelly DP, Saunders EM, Boddy L, Read DJ (2001) Rates and quantities of carbon flux to ectomycorrhizal mycelium following 14C pulse labelling of Pinus sylvestris seedlings: effects of litter patches and interaction with a wood-decomposer fungus. Tree Physiol 21:71–82PubMedGoogle Scholar
  12. Lindahl BD, Taylor AFS (2004) Occurrence of N-acetylhexosaminidase-encoding genes in ectomycorrhizal basidiomycetes. New Phytol 164:193–199CrossRefGoogle Scholar
  13. Lindahl B, Stenlid J, Olsson S, Finlay RD (1999) Translocation of 32P between interacting mycelia of a wood decomposing fungus and ectomycorrhizal fungi in microcosms systems. New Phytol 144:183–193CrossRefGoogle Scholar
  14. Lindahl B, Stenlid J, Finlay RD (2001) Effects of resource availability on mycelial interactions and 32P-transfer between a saprotrophic and an ectomycorrhizal fungus in soil microcosms. FEMS Microbiol Ecol 38:43–52CrossRefGoogle Scholar
  15. Lindahl BO, Taylor AFS, Finlay RD (2002) Defining nutritional constraints on carbon cycling in boreal forests—towards a less phytocentric perspective. Plant Soil 242:123–135CrossRefGoogle Scholar
  16. Merrill W, Cowling EB (1966) Role of nitrogen in wood deterioration. IV. Relationship of natural variation in nitrogen content in wood to its susceptibility to decay. Phytopathology 56:1324–1325Google Scholar
  17. Näsholm T, Ekblad A, Nordin A, Giesler R, Högberg M, Högberg P (1998) Boreal forest plants take up organic nitrogen. Nature 392:914–916CrossRefGoogle Scholar
  18. Persson T (1989) Role of soil animals in C and N mineralization. Plant Soil 115:241–245CrossRefGoogle Scholar
  19. Read DJ (1991) Mycorrhizas in ecosystems. Experientia 47:376–391CrossRefGoogle Scholar
  20. Read DJ, Perez-Moreno J (2003) Mycorrhizas and nutrient cycling in ecosystems—a journey towards relevance? New Phytol 157:475–492CrossRefGoogle Scholar
  21. Setälä H (1995) Growth of birch and pine seedlings in relation to grazing by soil fauna on ectomycorrhizal fungi. Ecology 76:1844–1851CrossRefGoogle Scholar
  22. Stevenson FJ (1982) Organic forms of soil nitrogen. In: Stevenson FJ (ed) Nitrogen in agricultural soils. American society of agronomy, Madison, USA, pp 67–122Google Scholar
  23. Tamm CO (1991) Nitrogen in terrestrial ecosystems. Ecological studies 81. Springer, Berlin Heidelberg New York, pp 46–49Google Scholar
  24. Tlalka M, Watkinson SC, Darrah PR, Fricker MD (2002) Continuous imaging of amino-acid translocation in intact mycelia of Phanerochaete velutina reveals rapid, pulsatile fluxes. New Phytol 153:173–184CrossRefGoogle Scholar
  25. Tlalka M, Hensman D, Darrah PR, Watkinson SC, Fricker MD (2003) Noncircadian oscillations in amino acid transport have complementary profiles in assimilatory and foraging hyphae of Phanerochaete velutina. New Phytol 158:325–335CrossRefGoogle Scholar
  26. Wallander H, Massicotte H, Nylund J-E (1997) Seasonal variation in ergosterol, chitin and protein in ectomycorrhizal roots collected in a Swedish pine forest. Soil Biol Biochem 29:45–53CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Håkan Wallander
    • 1
    Email author
  • Björn D. Lindahl
    • 2
  • Lars Ola Nilsson
    • 1
    • 3
  1. 1.Department of Microbial Ecology, Ecology BuildingLund UniversityLundSweden
  2. 2.Department of Forest Mycology and PathologySwedish University of Agricultural Sciences (SLU)UppsalaSweden
  3. 3.Danish Centre for ForestLandscape and Planning, KVLHørsholmDenmark

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