Mycorrhiza

, Volume 16, Issue 2, pp 137–142 | Cite as

In situ and in vitro colonization of Cathaya argyrophylla (Pinaceae) by ectomycorrhizal fungi

  • Lu-Min Vaario
  • Shu-Tang Xing
  • Zong-Qiang Xie
  • Zhi-Ming Lun
  • Xue Sun
  • Yu Hua Li
Original paper

Abstract

Cathaya argyrophylla, a critically endangered conifer, is found to grow at four isolated areas located in subtropical mountains of China. To examine the involvement and usefulness of mycorrhizas for sustaining the population of this tree, we compared the root system, morphology, and structure of mycorrhizal roots of C. argyrophylla, which were collected from a natural stand and an artificial stand, each grown at a different location. More mycorrhizal roots were found for trees from an artificial stand. The presence of extramatrical mycelium, mantle, and Hartig net revealed that C. argyrophylla formed an ectomycorrhizal association in both sampling sites. Starch granules were found in mycorrhizal roots collected only from a natural stand. The aseptic synthesis of C. argyrophylla and Cenococcum geophilum was established for the first time in vitro. Typical ectomycorrhizas formed on seedlings on RM medium containing 0.1 g/l glucose, 5 weeks after inoculation. By light microscopy, the synthesized mycorrhizas showed a thin mantle from which emanated extramatrical hyphae and highly branched Hartig net. A simple, rapid, and convenient mycorrhiza synthesis system was developed, which facilitates further studies on ectomycorrhizal development of C. argyrophylla.

Keywords

Cathaya argyrophylla Ectomycorrhizal fungi In vitro In situ Hartig net 

References

  1. Agerer R (1987–2002) Colour atlas of ectomycorrhizae, 1st–12th edn. Eihnorn-Verlag, Schwabisch-GmundGoogle Scholar
  2. Brundrett M, Bougher N, Dell B, Grove T, Malajczuk N (1996) Working with mycorrhizas in forestry and agriculture. Pirie Printers, Canberra, Australia, pp 173–216Google Scholar
  3. Carroll GC (1992) Fungal mutualism. In: Carroll GC, Wicklow DT (eds) The fungal community—its organization and role in the ecosystem, 2nd edn. Dekker, New York, pp 327–354Google Scholar
  4. Danielson RM, Pruden M (1989) The ectomycorrhizal status of urban spruce. Mycologia 81:335–341CrossRefGoogle Scholar
  5. Hall JR, Lyon AJE, Wang Y, Sinclair L (1998) Ectomycorrhizal fungi with edible fruiting bodies—2. Boletus edulis. Econ Bot 52:44–56Google Scholar
  6. Heslin MC, Douglas GC (1986) Synthesis of poplar mycorrhizas. Trans Br Mycol Soc 86:117–122CrossRefGoogle Scholar
  7. Hu YS, Wang FH, Chang YC (1976) On the comparative morphology and systematic position of Cathaya (Pinaceae). Acta Phytotaxon Sin 14(1):73–78 (in Chinese with English abstract)Google Scholar
  8. Hu YS, Wang FH (1984) Anatomical studies of Cathaya (Pinaceae). Am J Bot 71(5):727–735CrossRefGoogle Scholar
  9. Jordy MN, Azemar LS, Brun A, Botton B, Pargney JC (1998) Cytolocalization of glycogen, starch, and other insoluble polysaccharides during ontogeny of Paxillus involutusBetula pendula ectomycorrhizas. New Phytol 140(2):331–341CrossRefGoogle Scholar
  10. Martin F, Diez J, Dell B, Delaruelle C (2002) Phylogeography of the ectomycorrhizal Pisolithus species as inferred from nuclear ribosomal DNA ITS sequences. New Phytol 153(2):345–357CrossRefGoogle Scholar
  11. Marx DH (1969) The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic fungi infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology 59:153–163Google Scholar
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:437–497CrossRefGoogle Scholar
  13. Silba J (1986) An international census of the Coniferae. Phytologia memoir no 8. Moldenke HN and Moldenke AL, Corvallis, ORGoogle Scholar
  14. Smith SE, Read DJ (1997) Mycorrhizal symbiosis, 2nd edn. Academic, New YorkGoogle Scholar
  15. Standards of Forest Systems, P. R. China (2000) Forest soil analysis methods. Standards Press of China, Beijing, 334 pp (in Chinese)Google Scholar
  16. State Environmental Protection Administration of China (1992) China plant red data book. Science Press, Beijing, pp 64–65 (in Chinese)Google Scholar
  17. Stülten CH, Kong FX, Hampp R (1995) Isolation and regeneration of protoplasts from the ectomycorrhizal ascomycete Cenococcum geophilum Fr. Mycorrhiza 5:259–266Google Scholar
  18. Thomas GW (1996) Soil pH and soil acidity. In: Sparks DL (ed) Methods of soil analysis. Part 3—Chemical methods. SSSA special publication. Soil Science Society of America, Madison, WI, pp 475–490Google Scholar
  19. Trappe JM (1962) Fungus associates of ectotrophic mycorrhizae. Bot Rev 28:538–606Google Scholar
  20. Vaario LM, Guerin-Laguette A, Matsushita N, Suzuki K, Lapeyrie F (2002) Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants. Mycorrhiza 12:1–5PubMedCrossRefGoogle Scholar
  21. Vidakovic M (1991) Conifers: morphology and variation. Graficki Zavod Hrvatske, Croatia (translated from Croatian by Soljan M)Google Scholar
  22. Wang XQ (1990) Biology of Cathaya tree. Science Press, Beijing, 105 pp (in Chinese)Google Scholar
  23. Xie ZQ (1995) The studies on China own tree—Cathaya argyrophylla. Biodivers Sci 3(2):99–103 (in Chinese)Google Scholar
  24. Xie ZQ, Chen WL, Lu P, Hu D (1999) The demography and age structure of the endangered plant population of Cathaya argyrophylla. Acta Ecol Sin 19(4):523–528 (in Chinese with English abstract)Google Scholar
  25. Ying JS, Ma CG, Li LQ, Zhang ZS, Zhang WX (1983) Studies on the Cathaya communities. Acta Bot Sin 25(2):157–169 (in Chinese with English abstract)Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Lu-Min Vaario
    • 1
  • Shu-Tang Xing
    • 1
  • Zong-Qiang Xie
    • 2
  • Zhi-Ming Lun
    • 1
  • Xue Sun
    • 1
  • Yu Hua Li
    • 1
  1. 1.Research Institute of Flower BiotechnologyNortheast Forestry UniversityHarbinChina
  2. 2.The Laboratory of Quantitative Vegetation EcologyThe Institute of Botany, CASBeijingChina

Personalised recommendations