Mycorrhiza

, Volume 14, Issue 1, pp 1–10 | Cite as

Proteomics as a tool to monitor plant-microbe endosymbioses in the rhizosphere

Review
First Online:
Received:
Accepted:

Abstract

In recent years, outstanding molecular approaches have been used to investigate genes and functions involved in plant-microbe endosymbioses. In this review, we outline the use of proteomic analysis, based on two-dimensional electrophoresis and mass spectrometry, to characterize symbiosis-related proteins. During the last decade, proteomics succeeded in identifying about 400 proteins associated with the development and functioning of both mycorrhizal and rhizobial symbioses. Further progress in prefractionation procedures is expected to allow the detection of symbiotic proteins showing low abundance or being present in certain cell compartments.

Keywords

Mycorrhiza Nitrogen-fixing symbiosis Proteins Two-dimensional electrophoresis Mass spectrometry 
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Notes

Acknowledgements

The authors would like to thank Dr G. Recorbet for critically reading the manuscript, and G. Bestel-Corre thanks ADEME and Bourgogne Technologie for funding her PhD thesis.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • G. Bestel-Corre
    • 1
    • 2
  • E. Dumas-Gaudot
    • 1
  • S. Gianinazzi
    • 1
  1. 1.UMR 1088 INRA/CNRS 5184/UB (Plante-Microbe-Environnement)INRA-CMSEDijonFrance
  2. 2.Metabolic ExplorerSaint BeauzireFrance

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