, Volume 14, Issue 5, pp 295–306 | Cite as

Characterization and spatial distribution of ectomycorrhizas colonizing aspen clones released in an experimental field

  • Michael Kaldorf
  • Carsten Renker
  • Matthias Fladung
  • François Buscot
Original Paper


Ectomycorrhizas (EM) from aspen clones released on an experimental field were characterized by morphotyping, restriction analysis and internal transcribed spacer (ITS) sequencing. In addition, their community structure and spatial distribution was analyzed. Among the 23 observed morphotypes, six mycobionts dominated, forming roughly 90% of all ectomycorrhizas: Cenococcum geophilum, Laccaria sp., Phialocephala fortinii, two different Thelephoraceae, and one member of the Pezizales. The three most common morphotypes had an even spatial distribution, reflecting the high degree of homogeneity of the experimental field. The distribution of three other morphotypes was correlated with the distances to the spruce forest and deciduous trees bordering the experimental field. These two patterns allowed two invasion strategies of ectomycorrhizal fungi (EMF) to be recognized, the success of which depends on adaptation of the EMF to local ecological conditions.


Ectomycorrhiza ITS-sequences Morphotyping Spatial patterns Populus 



This work was kindly supported by a grant from the Federal Ministry of Education and Research, Germany (Grant number: 0311387). We wish to thank Dr. J. Perner for his help with statistical analysis and T. Spribille for critical reading of the manuscript.


  1. Addy HD, Hambleton S, Currah RS (2000) Distribution and molecular characterization of the root endophyte Phialocephala fortinii along an environmental gradient in the boreal forest of Alberta. Mycol Res 104:1213–1221CrossRefGoogle Scholar
  2. Agerer R (1991) Characterization of ectomycorrhiza. In: Norris JR, Read DJ, Varma AK (eds), Methods in microbiology, vol. 23. Academic Press, London, pp 25–73Google Scholar
  3. Agerer R (1987–1998) Colour atlas of ectomycorrhizae. Einhorn, Schwäbisch GmündGoogle Scholar
  4. Agerer R (2001) Exploration types of ectomycorrhizae. A proposal to classify ectomycorrhizal mycelial systems according to their patterns of differentiation and putative ecological importance. Mycorrhiza 11:107–114CrossRefGoogle Scholar
  5. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402PubMedGoogle Scholar
  6. Bruns TD (1995) Thoughts on the processes that maintain local species diversity of ectomycorrhizal fungi. Plant Soil 170:63–73Google Scholar
  7. Buscot F, Munch JC, Charcosset JY, Gardes M, Nehls U, Hampp R (2000) Recent advances in exploring physiology and biodiversity of ectomycorrhizas highlight the functioning of these symbioses in ecosystems. FEMS Microbiol Rev 24:601–614Google Scholar
  8. Cullings KW, Vogler DR, Parker VT, Finley SK (2000) Ectomycorrhizal specificity patterns in a mixed Pinus contorta and Picea engelmannii forest in Yellowstone National Park. Appl Environ Microbiol 66:4988–4991CrossRefPubMedGoogle Scholar
  9. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  10. Fattorini M, Halle S (in press) The dynamic environmental filter model: how do filtering effects change in assembling communities after disturbance? In: Temperton VM, Hobbs RJ, Nuttle TJ, Halle S (eds) Assembly rules and restoration ecology—bridging the gap between theory and practice. Island Press, Calif.Google Scholar
  11. Fiore-Donno A-M, Martin F (2001) Populations of ectomycorrhizal Laccaria amethystina and Xerocomus spp. show contrasting colonization patterns in a mixed forest. New Phytol 152:533–542CrossRefGoogle Scholar
  12. Fladung M, Ahuja MR (1996) Gene transfer in aspen. In: Schmidt ER, Hankeln T (eds) Transgenic organisms and biosafety. Springer, Berlin Heidelberg New YorkGoogle Scholar
  13. Fladung M, Muhs H-J (2000) In: Umweltbundesamt (ed) Field release with Populus tremula (rolC-gene) in Großhansdorf. Humboldt-Universität Berlin, pp 40–45Google Scholar
  14. Fladung M, Kumar S, Ahuja MR (1997) Genetic transformation of Populus genotypes with different chimaeric gene constructs: transformation efficiency and molecular analysis. Transgen Res 6:111–121CrossRefGoogle Scholar
  15. Gardes M, White TJ, Fortin JA, Bruns TD, Taylor JW (1991) Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA. Can J Bot 69:180–190Google Scholar
  16. Hashimoto Y, Hyakumachi M (2000) Quantities and types of ectomycorrhizal and endophytic fungi associated with Betula platyphylla var. japonica seedlings during the initial stage of establishment of vegetation after disturbance. Ecol Res 15:21–31CrossRefGoogle Scholar
  17. Horton TR, Bruns TD (2001) The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Mol Ecol 10:1855–1871PubMedGoogle Scholar
  18. Jonsson L, Dahlberg A, Nilsson M-C, Zackrisson O, Kårén O (1999) Ectomycorrhizal fungal communities in late-successional Swedish boreal forests, and their composition following wildfire. Mol Ecol 8:205–215CrossRefGoogle Scholar
  19. Jumpponen A, Mattson KG, Trappe JM (1998) Mycorrhizal functioning of Phialocephala fortinii with Pinus contorta on glacier forefront soil: interactions with soil nitrogen and organic matter. Mycorrhiza 7:261–265CrossRefGoogle Scholar
  20. Kaldorf M, Fladung M, Muhs H-J, Buscot F (2002) Mycorrhizal colonization of transgenic aspen in a field trial. Planta 214:653–660CrossRefPubMedGoogle Scholar
  21. Kårén O, Nylund J-E (1997) Effects of ammonium sulphate on the community structure and biomass of ectomycorrhizal fungi in a Norway spruce stand in southwestern Sweden. Can J Bot 75:1628–1642Google Scholar
  22. Kovács G, Pausch M, Urban A (2000) Diversity of ectomycorrhizal morphotypes and oak decline. Phyton 40:109–116Google Scholar
  23. Lilleskov EA, Fahey TJ, Horton TR, Lovett GM (2002) Belowground ectomycorrhizal fungal community change over a nitrogen deposition gradient in Alaska. Ecology 83:104–115Google Scholar
  24. Mason PA, Wilson J, Last FT, Walker C (1983) The concept of succession in relation to the spread of sheathing mycorrhizal fungi on inoculated tree seedlings growing in unsterile soils. Plant Soil 71:247–256Google Scholar
  25. Molina R, Massicotte H, Trappe JM (1992) Specificity phenomena in mycorrhizal symbioses: community-ecological consequences and practical implications. In: Allen MF (ed) Mycorrhizal functioning: an integrative plant-fungal process. Chapman & Hall, New York, pp 357–423Google Scholar
  26. Pigott CD (1982) Survival of mycorrhiza formed by Cenococcum geophilum Fr. in dry soils. New Phytol 92:513–517Google Scholar
  27. Pritsch K, Boyle H, Munch J-C, Buscot F (1997) Characterization and identification of black alder ectomycorrhizas by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS). New Phytol 137:357–369Google Scholar
  28. Sanchez F, Honrubia M, Torres P (2001) Effects of pH, water stress and temperature on in vitro cultures of ectomycorrhizal fungi from Mediterranean forests. Crypt Mycol 22:243–258CrossRefGoogle Scholar
  29. Schmülling T, Röhrig H, Pilz S, Walden R, Schell J (1993) Restoration of fertility by antisense RNA in genetically engineered male sterile tobacco plants. Mol Gen Genet 237:385–394PubMedGoogle Scholar
  30. Smith SE, Read DJ (1997) Mycorrhizal symbiosis, 2nd edn. Academic Press, San DiegoGoogle Scholar
  31. Spena A, Schmülling T, Koncz C, Schell J (1987) Independent and synergistic activity of rolA, B and C loci in stimulating abnormal growth in plants. EMBO J 6:3891–3899Google Scholar
  32. Unestam T, Sun Y-P (1995) Extramatrical structures of hydrophobic and hydrophilic ectomycorrhizal fungi. Mycorrhiza 5:301–311CrossRefGoogle Scholar
  33. Wang CJK, Wilcox HE (1985) New species of ectendomycorrhizal and pseudomycorrhizal fungi: Phialophora finlandia, Chloridium paucisporum, and Phialocephala fortinii. Mycologia 77:951–958Google Scholar
  34. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DM, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar
  35. Wu B, Nara K, Hogetsu T (1999) Competition between ectomycorrhizal fungi colonizing Pinus densiflora. Mycorrhiza 9:151–159CrossRefGoogle Scholar
  36. Wurzburger N, Bledsoe CS (2001) Comparison of ericoid and ectomycorrhizal colonization and ectomycorrhizal morphotypes in mixed conifer and pygmy forests on the northern California coast. Can J Bot 79:1202–1210CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Michael Kaldorf
    • 1
    • 2
    • 3
  • Carsten Renker
    • 1
    • 4
  • Matthias Fladung
    • 2
  • François Buscot
    • 1
    • 5
  1. 1.Institute of Ecology, Department of Environmental SciencesUniversity of JenaJenaGermany
  2. 2.Federal Research Center for Forestry and Forest ProductsInstitute for Forest Genetics and Forest Tree BreedingGroßhansdorfGermany
  3. 3.Institute of Botany, Department of Terrestrial EcologyUniversity of LeipzigLeipzigGermany
  4. 4.Institute of Botany, Department of Terrestrial EcologyUniversity of LeipzigLeipzigGermany
  5. 5.Institute of Botany, Department of Terrestrial EcologyUniversity of LeipzigLeipzigGermany

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