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Mycorrhiza

, Volume 14, Issue 3, pp 203–207 | Cite as

Comparison of 233U and 33P uptake and translocation by the arbuscular mycorrhizal fungus Glomus intraradices in root organ culture conditions

  • Gervais Rufyikiri
  • Stéphane Declerck
  • Yves Thiry
Original Paper

Abstract

This study aimed to quantify and compare 233U and 33P uptake and translocation by hyphae of the arbuscular mycorrhizal (AM) fungus Glomus intraradices in root organ culture conditions with transformed carrot (Daucus carota L.) roots as host. Mycorrhizal roots were grown in two-compartment Petri dishes to spatially separate a root compartment (RC) and a hyphal compartment (HC). The HC was labelled with 8.33 Bq 233U ml–1 and 13.33 Bq 33P ml–1. After 2 weeks contact between hyphae and the labelled solution, 233U and 33P activities were measured in the RC and in the HC. 233U and 33P were taken up by the extraradical AM mycelium grown in the HC and this uptake represented 4.4% and 16% of the initial isotope supply, respectively. The translocation into roots developing in the RC via hyphae accounted for 5.9% and 72% of the initial isotope supply, respectively. Thus, both uptake and translocation were much higher for 33P than for 233U. This suggests (1) the existence in hyphal tissues of efficient mechanisms limiting the uptake and translocation of non-essential elements such as U, and (2) that the hyphae have a higher sequestration than translocation function for U, and the converse for P.

Keywords

Arbuscular mycorrhizal fungus Ri T-DNA transformed roots Uranium Phosphorus Uptake 

Notes

Acknowledgements

This work was carried out while G. R. was holding a Postdoctoral Research Fellowship from the Belgian Nuclear Research Centre (SCK·CEN). The research was fully embodied in the EU-MYRRH project contract FIGE-CT-2000–00014 "Use of mycorrhizal fungi for the phytostabilization of radio-contaminated environments". S. D. gratefully acknowledges financial support from the Belgian Federal Office for Scientific, Technical and Cultural Affairs (OSTC, contract BCCM C2/10/007) and thanks the director of MUCL for the facilities provided and for continuous encouragement.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Gervais Rufyikiri
    • 1
  • Stéphane Declerck
    • 2
  • Yves Thiry
    • 1
  1. 1.Radiation Protection Research Department, Radioecology SectionBelgian Nuclear Research Centre (SCK·CEN) MolBelgium
  2. 2.Unité de microbiologieMycothèque de l'Université catholique de Louvain (MUCL) Louvain-la-NeuveBelgium

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