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Mycorrhiza

, Volume 13, Issue 4, pp 233–236 | Cite as

Glomeromycota rRNA genes—the diversity of myths?

  • A. Schüßler
  • D. Schwarzott
  • C. Walker
Comment

We are writing this to further elaborate on some aspects of published work on rRNA gene diversity among arbuscular mycorrhizal (AM) fungi (most of the members of the Glomeromycota), which is based in part on unfounded assumptions about their general genetic structure raised in a Comment by Clapp et al. (2002).

The first, least serious but perhaps most confusing aspect is the reference to the Glomales in the title of their Comment. The intended reference was to the AM fungi in general, which are now placed in the Glomeromycota. The term Glomerales (formerly orthographically incorrectly termed Glomales) now represents one of four orders within the phylum, and its use in a broad sense may result in lack of clarity. The taxonomic changes have been adopted also by the main sequence databases (EMBL, NCBI). The BEG culture collection has changed its name recently to 'International Bank for the Glomeromycota'.

We now address in more detail the main points discussed by Clapp et al. (2002) as...

Keywords

Arbuscular Mycorrhizal Trap Culture Taxonomic Change Contaminant Sequence Mycoparasitic Fungus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Ames RN, Schneider RW (1979) Entrophospora, a new genus in the Endogonaceae. Mycotaxon 8:347–352Google Scholar
  2. Chen DM, Cairney WG (2002) Investigation of the influence of prescribed burning on ITS profiles of ectomycorrhizal and other soil fungi at three Australian sclerophyll forest sites. Mycol Res 106:532–540CrossRefGoogle Scholar
  3. Clapp JP, Rodriguez A, Dodd JC (2001) Inter- and intra-isolate rRNA large subunit variation in Glomus coronatum spores. New Phytol 149:539–554Google Scholar
  4. Clapp JP, Rodriguez A, Dodd JC (2002) Glomales rRNA gene diversity—all that glistens is not necessarily glomalean? Mycorrhiza 12:269–270Google Scholar
  5. Jansa J, Mozafar A, Banke S, McDonald BA, Frossard E (2002) Intra- and intersporal diversity of ITS rDNA sequences in Glomus intraradices assessed by cloning and sequencing, and by SSCP analysis. Mycol Res 106:670–681CrossRefGoogle Scholar
  6. Millner PD, Mulbry WW, Reynolds SL (2001) Taxon-specific oligonucleotide primers for detection of Glomus etunicatum. Mycorrhiza 10:259–265Google Scholar
  7. O'Donnell K, Lutzoni FM, Ward TJ, Benny GL (2001) Evolutionary relationships among mucoralean fungi (Zygomycota): evidence for family polyphyly on a large scale. Mycologia 93:286–296Google Scholar
  8. Pringle A, Moncalvo J-M, Vilgalys R (2000) High levels of variation in ribosomal DNA sequences within and among spores of a natural population of the arbuscular mycorrhizal fungus Acaulospora colossica. Mycologia 92:259–268Google Scholar
  9. Redecker D, Hijri M, Dulieu H, Sanders IR (1999) Phylogenetic analysis of a dataset of fungal 5.8S rDNA sequences shows that highly divergent copies of Internal Transcribed Spacers reported from Scutellospora castanea are of Ascomycete origin. Fungal Genet Biol 28:238–244PubMedGoogle Scholar
  10. Rodriguez A, Dougall T, Dodd JC, Clapp JP (2001) The large subunit ribosomal RNA genes of Entrophospora infrequens comprise sequences related to two different glomalean families. New Phytol 152:159–167Google Scholar
  11. Schüßler A (1999) Glomales SSU rRNA gene diversity. New Phytol 144:205–207CrossRefGoogle Scholar
  12. Schüßler A, Gehrig H, Schwarzott D, Walker C (2001a) Analysis of partial Glomales SSU rRNA genes: implications for primer design and phylogeny. Mycol Res 105:5–15Google Scholar
  13. Schüßler A, Schwarzott D, Walker C (2001b) A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycol Res 105:1413–1421Google Scholar
  14. Schwarzott D, Walker C, Schüßler A (2001) Glomus, the largest genus of the arbuscular mycorrhizal fungi (Glomales), is non-monophyletic. Mol Phylogenet Evol 21:190–197PubMedGoogle Scholar
  15. Simon L, Lalonde M, Bruns TD (1992) Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl Environ Microbiol 58:291–295PubMedGoogle Scholar
  16. Walker C, Vestberg M (1994) A simple and inexpensive method for producing and maintaining closed pot cultures of arbuscular mycorrhizal fungi. Agric Sci Finland 3:233–240Google Scholar
  17. Walker C, Vestberg M (1998) Synonymy amongst the arbuscular mycorrhizal fungi: Glomus claroideum, G. maculosum, G. multisubstensum and G. fistulosum. Ann Bot 82:601–624CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Institute of Botany, FB 10TU DarmstadtDarmstadtGermany
  2. 2.Biological Research and Imaging LaboratoryNew MiltonUK

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