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Mycorrhiza

, Volume 12, Issue 1, pp 19–27 | Cite as

SCAR markers to detect mycorrhizas of an American Laccaria bicolor strain inoculated in European Douglas-fir plantations

  • Jean Weber
  • Jesús Díez
  • Marc-André Selosse
  • Denis Tagu
  • François Tacon
Original Paper

Abstract.

The American strain S238N of the ectomycorrhizal fungus Laccaria bicolor (Maire) Orton has been used to inoculate Douglas-fir [Pseudotsuga menziesii (Mir.) Franco] plantations in France over the last two decades. Laccaria fruit bodies are scarce in mature plantations, which precludes further assessment of its persistence by fruit body surveys. Our objective was to develop new markers to identify this strain and its eventual non-fruiting progeny on root tips. We converted nine random amplified polymorphic DNA markers into sequence characterized amplified region (SCAR) markers. Two of these SCAR markers enabled us to detect S238N on roots of seedlings and mature trees. No amplification of non-fungal (host plant, bacterial, etc.) DNA was observed. Moreover, both SCARs were amplified from Laccaria-like mycorrhizas in a Douglas-fir plantation inoculated 14 years ago, demonstrating the long-term persistence of the inoculant strain. We also obtained a SCAR marker to detect one strain of European origin (L. bicolor 81306), indicating that SCARs are potential markers to type the naturally occurring genets. Thus, SCAR markers are of great value in studying the persistence of inoculant strains and the effects on local populations of introducing foreign strains.

Douglas fir Ectomycorrhizas Laccaria bicolor Random amplified polymorphic DNA Sequence characterized amplified region 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Jean Weber
    • 2
  • Jesús Díez
    • 1
  • Marc-André Selosse
    • 3
  • Denis Tagu
    • 1
  • François Tacon
    • 1
  1. 1.Unité Mixte de Recherche INRA-UHP, Interactions Arbres/Micro-organismes, INRA-Nancy, 54280 Champenoux, FranceFrance
  2. 2.National Institute of Education, Nanyang Technological University, Natural Science Division, Biology Section, 1 Nanyang walk, Singapore 637616Singapore
  3. 3.Institut de Systématique (IFR CNRS 1541), Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75005 Paris, FranceFrance

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