Advertisement

A novel microfluidic high-throughput resistive pulse sensing device for cells analysis

  • Nastaran Khodaparastasgarabad
  • Armin Mohebbi
  • Cavus Falamaki
Technical Paper
  • 6 Downloads

Abstract

Microfluidic impedance-based devices offer a simple method for counting and sizing particles and cells in fields of biomedical research and clinical diagnosis. In this work, we present design, fabrication and operational characteristics of a novel high throughput original MEMS-based Coulter counter. This microfluidic device possesses two sub channels including two pairs of coplanar Au/Cr electrodes in each channels which allows double detection of the particles simultaneously and increases the throughput. The present design provides minimizing the cross talk and obviating the need for hydrodynamic focusing of the sample particles by adjusting Y shape insulation obstacle in direction of flow. Moreover, reducing coincidence events and removing electrode polarization effect were purposed by applying optimum sizes for electrodes considering the ease of fabrication and low costs. The reliability of the novel device was evaluated for polystyrene particles and cancer cells in conductive solutions. Results, which were recorded as relative resistance pulses across four sensing zones, illustrate the capability of the double-channel proposed device in detecting, counting and sizing 10 and 20 µm polystyrene particles. The superiority of present design was proved by relative counting error of below 3 and 11% for the 10 µm and 20 µm particles, respectively and a throughput of hundreds particles per second. Aiming at demonstrating the functionality of the proposed device in the biomedical area, counting of SP2/0 cells was performed. The measured counting outputs for cells in the size range of 5.63–17.6 µm were validated with results of hemocytometer cell counter, with relative error less than 7%.

Notes

References

  1. Chen Y et al (2015) Portable Coulter counter with vertical through-holes for high-throughput applications. Sens Actuators B Chem 213:375–381CrossRefGoogle Scholar
  2. Choi H et al (2014) A flow cytometry-based submicron-sized bacterial detection system using a movable virtual wall. Lab Chip 14:2327–2333CrossRefGoogle Scholar
  3. Coulter WH (1956) High-speed automatic blood cell counter and size analyzer. In: Preliminary draft of a talk presented before the national electronics conference, ChicagoGoogle Scholar
  4. DeBlois R, Bean C (1970) Counting and sizing of submicron particles by the resistive pulse technique. Rev Sci Instrum 41:909–916CrossRefGoogle Scholar
  5. Holmes D, Morgan H (2010) Single cell impedance cytometry for identification and counting of CD4 T-cells in human blood using impedance labels. Anal Chem 82:1455–1461CrossRefGoogle Scholar
  6. Jagtiani AV (2011) Novel multiplexed Coulter counters for high throughput parallel analysis of microparticles. The University of Akron, AkronGoogle Scholar
  7. Jagtiani AV, Zhe J, Hu J, Carletta J (2006) Detection and counting of micro-scale particles and pollen using a multi-aperture Coulter counter. Meas Sci Technol 17:1706CrossRefGoogle Scholar
  8. Lin D-S et al (2000) Comparison of hemocytometer leukocyte counts and standard urinalyses for predicting urinary tract infections in febrile infants. Pediatr Infect Dis J 19:223–227CrossRefGoogle Scholar
  9. Mei Z, Cho SH, Zhang A, Dai J, Wu T-F, Lo Y-H (2012) Counting leukocytes from whole blood using a lab-on-a-chip Coulter counter. In: Engineering in medicine and biology society (EMBC), 2012 Annual international conference of the IEEE. IEEE, pp 6277–6280Google Scholar
  10. Roberts K, Parmeswaran M, Moore M, Muller RS (1999) A silicon microfabricated aperture for counting cells using the aperture impedance technique. In: 1999 IEEE Canadian conference on electrical and computer engineering. IEEE, pp 1668–1673Google Scholar
  11. Saleh OA (2003) A novel resistive pulse sensor for biological measurements. Princeton University, PrincetonGoogle Scholar
  12. Saleh O, Sohn L (2001) Quantitative sensing of nanoscale colloids using a microchip Coulter counter. Rev Sci Instrum 72:4449–4451CrossRefGoogle Scholar
  13. Satake D, Ebi H, Oku N, Matsuda K, Takao H, Ashiki M, Ishida M (2002) A sensor for blood cell counter using MEMS technology. Sens Actuators B Chem 83:77–81CrossRefGoogle Scholar
  14. Schrum DP, Culbertson CT, Jacobson SC, Ramsey JM (1999) Microchip flow cytometry using electrokinetic focusing. Anal Chem 71:4173–4177CrossRefGoogle Scholar
  15. Wu Y, Benson JD, Critser JK, Almasri M (2010) Note: microelectromechanical systems Coulter counter for cell monitoring and counting. Rev Sci Instrum 81:076103CrossRefGoogle Scholar
  16. Yi C, Li C-W, Ji S, Yang M (2006) Microfluidics technology for manipulation and analysis of biological cells. Anal Chim Acta 560:1–23CrossRefGoogle Scholar
  17. Zhang Z, Zhe J, Chandra S, Hu J (2005) An electronic pollen detection method using Coulter counting principle. Atmos Environ 39:5446–5453CrossRefGoogle Scholar
  18. Zhe J, Jagtiani A, Dutta P, Hu J, Carletta J (2007) A micromachined high throughput Coulter counter for bioparticle detection and counting. J Micromech Microeng 17:304CrossRefGoogle Scholar
  19. Zheng S, Nandra MS, Tai Y-C (2007) Human blood cell sensing with platinum black electroplated impedance sensor. In: 2nd IEEE international conference on nano/micro engineered and molecular systems, 2007. NEMS’07. IEEE, pp 520–523Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Chemical Engineering DepartmentAmirkabir University of TechnologyTehranIran

Personalised recommendations