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Microsystem Technologies

, Volume 25, Issue 9, pp 3339–3355 | Cite as

Fabrication of three-dimensional mPEG-PCL-mPEG scaffolds combined with cell-laden gelatin methacrylate (GelMA) hydrogels using thermal extrusion coupled with photo curable technique

  • Jianghui Dong
  • Yu-Da Yang
  • Liping WangEmail author
  • Cho-Pei JiangEmail author
Technical Paper

Abstract

It has remained a great challenge to design a tissue engineering scaffold for tissue regeneration, which should be suitable for cell adhesion, proliferation and differentiation. One possible solution may be to fabricate the scaffolds with the stable mechanical property, controllable pore size and good interconnectivity, and allowing homogenous cell distribution. This study described the key technology of fabricating three-dimensional (3D) mPEG-PCL-mPEG scaffolds combined with cell-laden gelatin methacrylate (GelMA) hydrogels. Firstly, a dual-nozzle 3D printing system was successfully developed using thermal extrusion coupled with a photo curable technique. Then, the triblock material mPEG-PCL-mPEG was synthesized and evaluated. Subsequently, the fabricated 3D mPEG-PCL-mPEG scaffolds were injected with cell-laden GelMA hydrogels. Finally, the mPEG-PCL-mPEG scaffolds were evaluated. The evaluation results showed that this 3D mPEG-PCL-mPEG scaffolds technology is a potentially powerful approach, which may be used in a variety of tissue engineering applications.

Notes

Acknowledgements

This work was supported by MOST of Taiwan grant (106-2221-E-150-001) and NSFC (Natural Science Foundation of China) project grant (No.81671928). LPW is supported by National Health and Medical Research Council (NHMRC) Fellowship (No. APP1158402), Channel 7 Children’s Research Foundation grant (No.181662), and NSFC (No.81671928).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Hand Surgery, Department of Plastic Reconstructive SurgeryNingbo No.6 HospitalNingboChina
  2. 2.School of Pharmacy and Medical Sciences, and UniSA Cancer Research InstituteUniversity of South AustraliaAdelaideAustralia
  3. 3.Department of Power Mechanical Engineering, Institution of Mechanical and Electro-Mechanical EngineeringNational Formosa UniversityYunlinTaiwan
  4. 4.Department of Mechanical EngineeringNational Taipei University of TechnologyTaipeiTaiwan

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