Microsystem Technologies

, Volume 25, Issue 2, pp 711–717 | Cite as

Nano-electrokinetic ion concentration in the ion enrichment zone

  • Junyao WangEmail author
  • Lu-lu Han
  • Zheng Xu
Technical Paper


To reveal the internal discipline and accurately identify the inflection point, the Poisson–Nernst–Planck equations combined with the Navier–Stokes equation are employed and a method based on the horizontal angle of the tangent line and the distance from a pointcut to the origin is presented. Utilizing a micromachining technique and a photopolymerization reaction, a polyacrylamide gel plug is integrated into a microchannel to form a micro–nanofluidic chip. With the chip, FITC concentration experiments are developed with the initial concentration of 10 nM. Experimental testing confirms the results and demonstrates that the concentration is increased at first and then decreased with the increasing of the electric potential. That variation trend should be attributed to a balance between the repulsive force and the electrophoresis effect. The research results also demonstrate that upon increasing the voltage, the horizontal angle first decreases and then increases corresponding with the reverse trend of the distance. For a given micro–nanochannel, the peak ion concentration can be obtained through decreasing the horizontal angle and increasing the distance. The results will help determine peak voltage.



This project is supported by National Natural Science Foundation of China (Grant no. 51505077), Project Agreement for Science and Technology Development of Jilin Province (20170520099JH), “13th Five” Science and Technology Research Projects of Jilin Provincial Education Department (201686) and Science and Technology Innovation Development Project of Jilin City (20166013, 20166012).


  1. Hong SA, Kim YJ, Kim SJ, Yang S (2018) Electrochemical detection of methylated DNA on a microfluidic chip with nanoelectrokinetic pre-concentration. Biosens Bioelectron 107:103–110CrossRefGoogle Scholar
  2. Kim P, Kim SJ, Han J, Suh KY (2009) Stabilization of ion concentration polarization using a heterogeneous nanoporous junction. Nano Lett 10:16–23CrossRefGoogle Scholar
  3. Kim SJ, Ko SH, Kang KH (2010) Direct seawater desalination by ion concentration polarization. Nat Nanotechnol 5:297–301CrossRefGoogle Scholar
  4. Kim SJ, Ko SH, Kwak R, Posner JD, Kang KH, Han J (2012) Multi-vortical flow inducing electrokinetic instability in ion concentration polarization layer. Nanoscale 4:7406–7410CrossRefGoogle Scholar
  5. Li CY, Wu ZQ, Yuan CG, Wang K, Xia XH (2015) Propagation of concentration polarization affecting ions transport in branching nanochannel array. Anal Chem 87:8194–8202CrossRefGoogle Scholar
  6. Liu JS, Qiao HC, Xu Z, Liu C, Wang JY, Du LQ, Zhang X, Wang LD (2012) Fabrication of planar nanofluidic channels in thermoplastic polymers by O2 plasma etching. Micro Nano Lett 7(2):159–162CrossRefGoogle Scholar
  7. Plecis A, Schoch RB, Renaud P (2005) Ionic transport phenomena in nanofluidics: experimental and theoretical study of the exclusion-enrichment effect on a chip. Nano Lett 5:1147–1155CrossRefGoogle Scholar
  8. Plecis A, Pallandre A, Haghiri-Gosnet AM (2011) Ionic and mass transport in micro–nanofluidic devices: a matter of volumic surface charge. Lab Chip 11:795–804CrossRefGoogle Scholar
  9. Pu Q, Yun J, Temkin H et al (2004) Ion-enrichment and ion-depletion effect of nanochannel structures. Nano Lett 4:1099–1103CrossRefGoogle Scholar
  10. Son SY, Lee S, Lee H, Kim SJ (2017) Engineered nanofluidic preconcentration devices by ion concentration polarization. Biochip J 10:251–261CrossRefGoogle Scholar
  11. Wang JY, Xu Z, Liu C, Liu JS, Liu YL, Wang LD, Yang WD (2012) Effects of electrophoresis and electroosmotic flow on ion enrichment in micro–nanofluidic preconcentrator. Microsyst Technol 18(1):97–102CrossRefGoogle Scholar
  12. Wang JY, Xu Z, Li YK, Liu C, Liu JS, Chen L, Du LQ, Wang LD (2013) Nanopore density effect of polyacrylamide gel plug on electrokinetic ion enrichment in a micro–nanofluidic chip. Appl Phys Lett 103(4):043103-1-5Google Scholar
  13. Xu Z, Wen JK, Liu C, Liu JS, Du LQ, Wang LD (2009) Research on forming and application of U-form glass micro–nanofluidic chip with long nanochannels. Microfluid Nanofluid 7(3):423–429CrossRefGoogle Scholar
  14. Xu Z, Wang JY, Hu SL, Lu JQ, Liu C, Liu JS (2016) Electrokinetic concentrating with a nanofluidic device for magnetic beads-based antigen–antibody immunoassay. Microsyst Technol 22(2):283–286CrossRefGoogle Scholar
  15. Yuan XC, Renaud L, Audry MC, Kleimann P (2015) Electrokinetic biomolecule preconcentration using xurography-based micro–nano–micro fluidic devices. Anal Chem 87(17):8695–8701CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Mechanic EngineeringNortheast Electric Power UniversityJilinChina
  2. 2.Key Laboratory for Micro/Nano Technology and System of Liaoning ProvinceDalian University of TechnologyDalianChina

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