Microsystem Technologies

, Volume 21, Issue 1, pp 41–48 | Cite as

Multichannel oscillatory-flow PCR micro-fluidic chip with controllable temperature gradient

Technical Paper
First Online:
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Abstract

This study presents a multichannel oscillatory-flow micro-fluidic chip for high-throughput polymerase chain reaction (PCR). The micro-fluidic chip consists of microheaters and temperature sensors integrated on silicon substrates and parallel microchannels fabricated with glass/PDMS laminates. In order to achieve multiplex thermal protocols simultaneously, the micro-fluidic chip combines uniform resistors and gradually changed resistors on the heater chips. By regulating the heating power of the gradually changed resistors, a controllable temperature gradient was obtained on the micro-fluidic chip for same/different annealing temperature. Then the temperature gradient was investigated in the experiment. The results showed that the temperature difference could change from 0 to 20 °C in the range of about 50–70 °C with low power consumption. Finally, the performance of the developed micro-fluidic chip was successfully demonstrated by amplifying a detection gene associated with hepatitis B virus (HBV) at same and different annealing temperature, respectively. The developed micro-fluidic chip may provide a useful platform for high-throughput PCR amplification.

Keywords

Polymerase Chain Reaction Heating Power Temperature Control System Polymerase Chain Reaction Tube Polymerase Chain Reaction Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This work is supported by the National Science Foundation of China under the Grant Nos. 61176113, the Program for Changjiang Scholars and Innovative Research Team in University (IRT1033).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.State Key Laboratory for Manufacturing Systems EngineeringXi’an Jiaotong UniversityXi’anChina

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