The development of a novel microfluidic device that includes a circular microchannel and integrated electrodes for DNA electrophoresis is reported. The geometry of the separation channel and the arrangement of the embedded electrodes provide several advantages in relation to conventional linear microchannels. The paper describe the design, modeling, construction, and testing of the micro device. Numerical simulations were used to investigate the electric field into the microchannel. In addition, the electrophoretic transport of DNA samples was studied under different voltage configurations. The experiments reported show the functionality of the device, and illustrate interesting features of DNA transport.
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CLAB thanks the financial support from CONICET (PIP 0317) and Universidad Nacional del Litoral (CAI + D65/328), Argentina. We would like to thank M.J. Dieguez, F. Sacco and E.M. Salmoral for technical advice and discussion.
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Supplementary material S1 The video shows a fluorescence microscopy of DNA migration from a negative electrode. A volume element of DNA was situated on electrode 2, and then a potential difference of 3 V was applied between electrodes 1 and 2, as well as between electrodes 2 and 3, as shown schematically in Fig. 13a. (MPG 1076 kb)
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Lerner, B., Kler, P.A., Ordoñez Arias, A.F. et al. A circular microchannel with integrated electrodes for DNA electrophoresis. Microsyst Technol 19, 733–742 (2013) doi:10.1007/s00542-012-1678-7
- Field Line
- Separation Channel
- Circular Channel
- Injection Zone