Journal of Anesthesia

, Volume 15, Issue 1, pp 29–32

Hyperbaric hyperoxia suppresses growth of Staphylococcus aureus, including methicillin-resistant strains

  • Isao Tsuneyoshi
  • Walter A. Boyle III
  • Yuichi Kanmura
  • Toushiro Fujimoto

Abstract

Purpose. We investigated the effects of increased oxygen tension on the in vitro growth of Staphylococcus aureus (MRSA), methicillin-sensitive Staphylococcus aureus (MSSA), and Escherichia coli (E. coli).

Methods. The effects of oxygen tension [normobaric normoxia (21% O2 at 1 atm), normobaric hyperoxia (100% O2 at 1 atm), hyperbaric normoxia (21% O2 at 2 atm), and hyperbaric hyperoxia (100% O2 at 2 atm) on the in vitro growth of MRSA, MSSA, and E. coli were investigated by population analysis.

Results. Compared with normobaric normoxia, a 90-min exposure to hyperbaric hyperoxia significantly inhibited growth of both MRSA (by 25.0 ± 3.0%, mean ± SEM; P < 0.01) and MSSA (by 24.0 ± 3.3%; P < 0.01). Normobaric hyperoxia and hyperbaric normoxia were without effect. In contrast, the growth of E. coli was not affected by any of the above treatments.

Conclusion. Our results indicate that the bacterium S. aureus, including resistant strains, is susceptible to oxygen stress. The observation that relatively brief (90-min) treatment with hyperbaric hyperoxia is sufficient to produce significant growth inhibition suggests that hyperbaric hyperoxia may be useful in the treatment of serious staphylococcal infections.

Key words Methicillin-resistant Staphylococcus aureus Methicillin-sensitive Staphylococcus aureus Escherichia coli Hyperbaric oxygen 

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Copyright information

© Japan Society of Anesthesiology 2001

Authors and Affiliations

  • Isao Tsuneyoshi
    • 1
  • Walter A. Boyle III
    • 1
  • Yuichi Kanmura
    • 2
  • Toushiro Fujimoto
    • 3
  1. 1.Department of Anesthesiology, Washington University School of Medicine, 660 S. Euclid Avenue, Box 8054, St. Louis, MO 63110, USAUS
  2. 2.Department of Anesthesiology and Critical Care Medicine, Kagoshima University School of Medicine, 8-35-1 Sakuragaoka, Kagoshima 890-8520, JapanJP
  3. 3.Department of Medicine, Fujimoto Hospital, 17-4 Hayasucho, Miyakonojo, Miyazaki 885-0055, JapanJP

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