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Journal of Anesthesia

, Volume 32, Issue 6, pp 880–885 | Cite as

Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model

  • Kensuke Kobayashi
  • Soichiro Mimuro
  • Tsunehisa Sato
  • Atsushi Kobayashi
  • Shingo Kawashima
  • Hiroshi Makino
  • Matsuyuki Doi
  • Takasumi Katoh
  • Yoshiki Nakajima
Original Article

Abstract

Purpose

Heatstroke causes systemic inflammation, followed by vascular endothelial damage. The normal vascular endothelium is coated by endothelial glycocalyx (EGCX). Dexmedetomidine (DEX) has an anti-inflammatory effect, but there has been little investigation on the influence of heatstroke on EGCX and the effect of DEX on this condition. Therefore, we examined whether EGCX was disrupted in heatstroke and if DEX improved survival and preserves EGCX.

Methods

Anesthetized Wistar rats were randomly assigned to three groups: a DEX group treated with DEX (5 µg/kg/h) and 0.9% saline infused continuously at 10 ml/kg/h during heat exposure; a NSS group given 0.9% saline during heat exposure; and a SHAM group given 0.9% saline alone without heat exposure. Heatstroke was induced by exposure to an ambient temperature of 40 °C with relative humidity of 60%. The survival rate was assessed up to 2 h after the start of heat exposure. Plasma levels of syndecan-1 and the thickness of EGCX using electron microscopy were measured when the systolic blood pressure fell to less than 80 mmHg.

Results

The survival rate after 2 h of heat exposure was significantly higher in the DEX group compared to the NSS group (89% vs. 22%, P = 0.004). Plasma levels of syndecan-1 were 0.6 ± 1.3, 9.7 ± 5.9, and 2.1 ± 3.4 ng/ml in the SHAM, NSS and DEX groups, respectively (P = 0.013). The thickness of EGCX was significantly higher in the DEX group compared with the NSS group (P = 0.001).

Conclusions

EGCX was disrupted in heatstroke, and DEX improved survival and preserved EGCX.

Keywords

Dexmedetomidine Heatstroke Glycocalyx 

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Copyright information

© Japanese Society of Anesthesiologists 2018

Authors and Affiliations

  • Kensuke Kobayashi
    • 1
  • Soichiro Mimuro
    • 1
  • Tsunehisa Sato
    • 1
  • Atsushi Kobayashi
    • 1
  • Shingo Kawashima
    • 1
  • Hiroshi Makino
    • 1
  • Matsuyuki Doi
    • 1
  • Takasumi Katoh
    • 1
  • Yoshiki Nakajima
    • 1
  1. 1.Department of Anesthesiology and Intensive CareHamamatsu University School of MedicineHamamatsuJapan

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