Journal of Anesthesia

, Volume 19, Issue 3, pp 263–265 | Cite as

Opioid receptor stimulation does not affect cellular hypoxia-induced gene responses mediated by hypoxia-inducible factor 1 in cultured cell lines

  • Satoshi Takabuchi
  • Kiichi Hirota
  • Seiko Oda
  • Kenichiro Nishi
  • Tomoyuki Oda
  • Koh Shingu
  • Takehiko Adachi
  • Kazuhiko Fukuda
Article

Abstract

Hypoxia induces a series of adaptive physiological responses including gene inductions. Hypoxia-inducible factor 1 (HIF-1) is a master transcription factor that regulates hypoxia-induced gene expression to maintain homeostasis in the living body. Opioids are potent analgesic agents that are widely used in clinical practice. Therefore, we investigated the effect of opioids on HIF-1 activity. SH-SY5Y human neuronal cells, which express opioid receptors intrinsically, were cultured under 1% or 20% O2 conditions with or without treatment by DAGO, DPDPE, or U-50488, which are the selective agonists of µ-, δ-, and κ-opioid receptors, respectively. Expression of subunits of HIF-1, HIF-1α, and HIF-1β were examined by Western blot using specific antibodies. Expression of the HIF-1-dependent gene were investigated by reporter assay. None of the selective agonists of opioid receptors tested affected HIF-1 activation by hypoxia. Therefore, it is suggested that opioid receptor-mediated signals do not affect HIF-1-dependent cellular hypoxia-induced gene responses.

Key words

Hypoxia Hypoxia-inducible factor 1 (HIF-1) Opioid Guanine nucleotide-binding (G) protein-coupled receptor (GPCR) SH-SY5Y 

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Copyright information

© JSA 2005

Authors and Affiliations

  • Satoshi Takabuchi
    • 1
    • 2
  • Kiichi Hirota
    • 1
    • 2
  • Seiko Oda
    • 1
    • 2
  • Kenichiro Nishi
    • 1
    • 3
  • Tomoyuki Oda
    • 1
    • 4
  • Koh Shingu
    • 3
  • Takehiko Adachi
    • 1
  • Kazuhiko Fukuda
    • 2
  1. 1.Department of Anesthesia, The Tazuke Kofukai Medical Research InstituteKitano HospitalOsakaJapan
  2. 2.Department of Anesthesia, Kyoto University HospitalKyoto UniversityKyotoJapan
  3. 3.Department of AnesthesiologyKansai Medical UniversityOsakaJapan
  4. 4.Department of Cardiovascular Medicine, Graduate School of MedicineKyoto UniversityKyotoJapan

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