Journal of Gastroenterology

, Volume 33, Issue 3, pp 343–348 | Cite as

Telomere shortening in the colonic mucosa of patients with ulcerative colitis

  • Yoshitaka Kinouchi
  • Nobuo Hiwatashi
  • Masaki Chida
  • Fumio Nagashima
  • Sho Takagi
  • Hiroki Maekawa
  • Takayoshi Toyota

Abstract:

Telomere length in human somatic cells gradually decreases with the number of cell divisions and is regarded as a marker of somatic cell turnover. Mucosal cells of the affected colon show rapid turnover in individuals with active ulcerative colitis (UC). Telomere length was determined by Southern blot analysis of terminal restriction fragments (TRFs) from the colonic mucosa of 17 patients with UC in remission, two of whom showed dysplasia, and 17 control subjects without colitis. For each individual, mean TRF length was compared between rectal mucosa and unaffected cecal mucosa. The mean TRF length of the rectal mucosa was significantly less than that of cecal mucosa in UC patients (7.87 ± 0.36 kb versus 8.77 ± 0.21 kb; P = 0.0015, Wilcoxon signed rank test), whereas no significant difference was detected in the control subjects. The extent of telomere shortening was 10.6 ± 3.35% in UC patients, compared with 0.8 ± 0.64% in noncolitis controls (P = 0.0024, Mann-Whitney U-test). Four UC patients, two of whom had dysplasia, showed telomere shortening of more than 20% in the rectal mucosa. These observations suggest that telomere shortening in the colonic mucosa of individuals with UC may represent the history of mucosal inflammation during disease of long duration, and that it may contribute to aneuploidy in UC.

Key words: telomere ulcerative colitis terminal restriction fragment dysplasia aneuploidy 

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Copyright information

© Springer-Verlag Tokyo 1998

Authors and Affiliations

  • Yoshitaka Kinouchi
    • 1
  • Nobuo Hiwatashi
    • 1
  • Masaki Chida
    • 1
  • Fumio Nagashima
    • 1
  • Sho Takagi
    • 1
  • Hiroki Maekawa
    • 1
  • Takayoshi Toyota
    • 1
  1. 1.Third Department of Internal Medicine, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-77, JapanJP

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