Journal of Gastroenterology

, Volume 33, Issue 2, pp 189–195

Infiltration of peroxidase-producing eosinophils into the lamina propria of patients with ulcerative colitis

  • Hideki Nishitani
  • Masafumi Okabayashi
  • Masamichi Satomi
  • Takashi Shimoyama
  • Yoshitane Dohi

DOI: 10.1007/s005350050068

Cite this article as:
Nishitani, H., Okabayashi, M., Satomi, M. et al. J Gastroenterol (1998) 33: 189. doi:10.1007/s005350050068

Abstract:

Little information is available to explain the pathogenesis of ulcerative colitis (UC). In this study, we focused on eosinophils in the lamina propria of the mucosa of patients with UC in the active phase. Biopsy specimens were taken from 17 patients with UC in the active phase, 17 in the inactive phase, and 20 control patients, and submitted for histochemical staining for peroxidase and chloroacetate esterase for microscopic examination. Both peroxidase-producing and chloroacetate esterase-producing cells in the lamina propria increased markedly in the active phase (8.3 ± 3.1/0.01 mm2 and 6.6 ± 2.7/0.01 mm2, respectively), compared with values in the inactive phase (0.8 ± 0.6/0.01 mm2 and 1.3 ± 0.6/0.01 mm2) or in the controls (1.3 ± 0.8/0.01 mm2 and 1.3 ± 0.4/0.01 mm2). Triple staining for peroxidase, chloroacetate esterase, and nonspecific esterase in the specimens revealed that the peroxidase-producing cells constituted a different population from that of neutrophils, macrophages/monocytes, or basophils. A monoclonal antibody specific for eosinophil peroxidase stained almost all infiltrated peroxidase-producing cells. These results indicated that eosinophils with strong peroxidase activity had infiltrated the lamina propria in UC, suggesting an allergic background and the involvement of released peroxidase in the mucosal damage characteristic of UC.

Key words: ulcerative colitis eosinophils peroxidase 

Copyright information

© Springer-Verlag Tokyo 1998

Authors and Affiliations

  • Hideki Nishitani
    • 1
  • Masafumi Okabayashi
    • 1
  • Masamichi Satomi
    • 1
  • Takashi Shimoyama
    • 1
  • Yoshitane Dohi
    • 2
  1. 1.Fourth Department of Internal Medicine, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663, JapanJP
  2. 2.Department of Microbiology and Infectious Diseases, Division of Allied Health Sciences, Osaka University Medical School, Osaka, JapanJP

Personalised recommendations