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Characterization of fungal dysbiosis in Japanese patients with inflammatory bowel disease

  • Takayuki Imai
  • Ryo Inoue
  • Yuki Kawada
  • Yasuhiro Morita
  • Osamu Inatomi
  • Atsushi Nishida
  • Shigeki Bamba
  • Masahiro Kawahara
  • Akira Andoh
Original Article—Alimentary Tract

Abstract

Background and aims

There are no previous reports describing the fecal fungal microbiome of a Japanese population using advanced molecular techniques. In this study, we performed a molecular analysis on the fungal microbial community of a healthy Japanese population and patients with inflammatory bowel diseases (IBDs).

Patients and methods

Fecal samples were obtained from 18 patients with inactive ulcerative colitis (UC, n = 18), Crohn’s disease (CD, n = 20) and healthy volunteers (n = 20). Bacterial and fungal microbiome was analyzed by sequencing of 16S rRNA and the internal transcribed spacer (ITS) region, respectively.

Results

16S rRNA sequencing of the bacterial microbiome revealed that the α-diversity indicated by the Chao-1 and Shannon indices was significantly lower in CD patients compared to healthy controls and/or UC patients. Principal coordinate (PCo) analysis of the bacterial community revealed significant structural differences in microbiome among healthy controls, UC and CD patients (PERMANOVA P = 0.0001). ITS sequencing of the fungal microbiome indicated no significant differences in α-diversity between healthy controls and IBD patients. However, the overall structure of the fungal microbial community of CD patients was significantly different from those of healthy controls and UC patients (PERMANOVA = 0.03). At the genus level, the genus Saccharomyces was dominant and this was followed by the genus Sarocladium in healthy controls. The abundance of the genus Candida was significantly higher in CD patients than healthy controls and/or UC patients.

Conclusion

The fecal fungal microbiome of a Japanese population differed considerably from that of a Western population. We identified fungal dysbiosis in Japanese patients with IBD.

Keywords

Fungi Candida IBD 

Notes

Acknowledgements

This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (18K08002), a grant for the Intractable Diseases from the Ministry of Health, Labor and Welfare of Japan, and a grant from the Smoking Research Foundation.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest in this study.

Supplementary material

535_2018_1530_MOESM1_ESM.pptx (1.3 mb)
Supplementary material 1 (PPTX 1308 kb)

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Copyright information

© Japanese Society of Gastroenterology 2018

Authors and Affiliations

  • Takayuki Imai
    • 1
  • Ryo Inoue
    • 2
  • Yuki Kawada
    • 2
  • Yasuhiro Morita
    • 1
  • Osamu Inatomi
    • 1
  • Atsushi Nishida
    • 1
  • Shigeki Bamba
    • 1
  • Masahiro Kawahara
    • 1
  • Akira Andoh
    • 1
  1. 1.Department of MedicineShiga University of Medical ScienceOtsuJapan
  2. 2.Laboratory of Animal Science, Department of Agriculture and Life ScienceKyoto Prefectural UniversityKyotoJapan

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