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Journal of Gastroenterology

, Volume 54, Issue 5, pp 407–418 | Cite as

Deletion of IP3R1 by Pdgfrb-Cre in mice results in intestinal pseudo-obstruction and lethality

  • Hong Wang
  • Ran Jing
  • Christa Trexler
  • Yali Li
  • Huayuan Tang
  • Zhixiang Pan
  • Siting Zhu
  • Beili Zhao
  • Xi Fang
  • Jie Liu
  • Ju ChenEmail author
  • Kunfu OuyangEmail author
Original Article—Alimentary Tract

Abstract

Background

Inositol 1,4,5-trisphosphate receptors (IP3Rs) are a family of intracellular Ca2+ release channels located on the membrane of endoplasmic reticulum, which have been shown to play critical roles in various cellular and physiological functions. However, their function in regulating gastrointestinal (GI) tract motility in vivo remains unknown. Here, we investigated the physiological function of IP3R1 in the GI tract using genetically engineered mouse models.

Methods

Pdgfrb-Cre mice were bred with homozygous Itpr1 floxed (Itpr1f/f) mice to generate conditional IP3R1 knockout (pcR1KO) mice. Cell lineage tracing was used to determine where Pdgfrb-Cre-mediated gene deletion occurred in the GI tract. Isometric tension recording was used to measure the effects of IP3R1 deletion on muscle contraction.

Results

In the mouse GI tract, Itpr1 gene deletion by Pdgfrb-Cre occurred in smooth muscle cells, enteric neurons, and interstitial cells of Cajal. pcR1KO mice developed impaired GI motility, with prolonged whole-gut transit time and abdominal distention. pcR1KO mice also exhibited lethality as early as 8 weeks of age and 50% of pcR1KO mice were dead by 40 weeks after birth. The frequency of spontaneous contractions in colonic circular muscles was dramatically decreased and the amplitude of spontaneous contractions was increased in pcR1KO mice. Deletion of IP3R1 in the GI tract also reduced the contractile response to the muscarinic agonist, carbachol, as well as to electrical field stimulation. However, KCl-induced contraction and expression of smooth muscle-specific contractile genes were not significantly altered in pcR1KO mice.

Conclusions

Here, we provided a novel mouse model for impaired GI motility and demonstrated that IP3R1 plays a critical role in regulating physiological function of GI tract in vivo.

Keywords

IP3 receptor Ca2+ release channel Gut motility Intestinal pseudo-obstruction 

Notes

Acknowledgements

The work was supported by the National Science Foundation of China (31370823, 81700289, 31800767), the Guangdong Province Basic Research Foundation (2018A030310012), the Shenzhen Basic Research Foundation (KCYJ20160428154108239, KQJSCX20170330155020267, JCYJ20170818090044949, KQTD2015032709315529), and the National Institutes of Health (J.C. and F.X.). J.C. is the American Heart Association (AHA) Endowed Chair in Cardiovascular Research.

Author contribution

HW, RJ, YL, HT, ZP, SZ, and BZ performed research; KO, XF, and JC designed the research; KO, CT, XF, JL, and JC wrote the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Japanese Society of Gastroenterology 2018

Authors and Affiliations

  1. 1.Drug Discovery Center, State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate SchoolShenzhenChina
  2. 2.Xiangya HospitalCentral South UniversityChangshaChina
  3. 3.Department of MedicineUniversity of California-San DiegoLa JollaUSA
  4. 4.Department of Pathophysiology, School of MedicineShenzhen UniversityShenzhenChina

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