A genetic variant located in the miR-532-5p-binding site of TGFBR1 is associated with the colorectal cancer risk
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Genome-wide association studies have identified genes in the transforming growth factor-β (TGFβ) signaling pathway that are responsible for regulating carcinogenesis.
We searched for single-nucleotide polymorphisms (SNPs) located within 3′-untranslated regions (3′-UTRs) that might affect the ability of miRNAs to bind genes in the TGFβ pathway for further analysis. We used TaqMan technology to genotype these SNPs in a population-based case–control study of 1147 colorectal cancer patients and 1203 matched controls in a Chinese population.
The rs1590 variant of TGFBR1 exhibited a significant association with colorectal cancer risk. Compared with individuals carrying the rs1590 TT genotype, individuals carrying the GT/GG genotypes had a decreased risk of colorectal cancer [odd ratio (OR) = 0.82, 95% confidence interval (CI) = 0.68–0.97], which was more evident among older individuals with a family history of cancer. Luciferase assays confirmed that the rs1590 T allele altered the capacity of miR-532-5p to bind TGFBR1.
Based on these findings, the rs1590 variant in the 3′-UTR of TGFBR1 may contribute to the susceptibility to colorectal cancer, predominantly by altering miR-532-5p binding.
KeywordsTGFβ Colorectal cancer Genetic variants Risk
Genome-wide association study
Transforming growth factor-β
The authors alone are responsible for the content and writing of the article.
Jinfei Chen and Meilin Wang conceived and designed the experiments. Dongying Gu, Shuwei Li, and Mulong Du wrote the paper. Cuju Tang, Haiyan Chu, and Na Tong contributed reagents/materials/analysis tools. Dongying Gu, Zhengdong Zhang, and Jinfei Chen recruited samples. All authors reviewed the manuscript.
Compliance with ethical standards
Conflict of interest
The authors disclose no potential conflicts of interest.
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