Journal of Gastroenterology

, Volume 50, Issue 4, pp 480–490

miR-33a levels in hepatic and serum after chronic HBV-induced fibrosis

  • Chuan-Feng Huang
  • Cheng-Chao Sun
  • Fang Zhao
  • Ya-Dong Zhang
  • De-Jia Li
Original Article—Liver, Pancreas, and Biliary Tract

DOI: 10.1007/s00535-014-0986-3

Cite this article as:
Huang, CF., Sun, CC., Zhao, F. et al. J Gastroenterol (2015) 50: 480. doi:10.1007/s00535-014-0986-3

Abstract

Background

Chronic hepatitis B virus (HBV) infection, which can lead to hepatic disease, has become a critical national healthcare problem, and many people die each year as a result of HBV infection and its complications. Although microRNA-33a (miR-33a) is a novel modulator of lipid and cholesterol metabolism, the role of miR-33a in the hepatic fibrogenesis is still unknown. Here, we aimed to explore the roles and mechanisms of miR-33a in liver fibrosis.

Methods

miR-33a expression in whole liver and serum samples was measured from chronic hepatitis B (CHB) patients by quantitative real-time PCR (qRT-PCR). In addition, different murine hepatic fibrosis models were produced to consolidate the results in human tissue. Human and murine primary liver fibrosis-associated cells were isolated and treated with transforming growth factor-β1 (TGF-β1).

Results

miR-33a expression levels in liver tissue significantly increased with a fibrosis progression manner in the human liver. Furthermore, serum miR-33a levels associated positively with progressing process of hepatic fibrosis. miR-33a was in particular increased in hepatic stellate cells (HSC) than other liver fibrosis-associated cells. Stimulation of HSCs with TGF-β1 leads to a critical increase of miR-33a. Increasing miR-33a levels increased (whereas inhibiting miR-33a weakened) the activation role of TGF-β1 in LX-2 cells, which might be a potential mechanism through moderating Smad7 expression.

Conclusions

miR-33a may be a novel marker for HSC activation and hepatic fibrosis progress, suggesting a new therapeutic target in liver fibrosis.

Keywords

Liver fibrosis HBV Hepatic stellate cells microRNA 33a TGF-β1 

Copyright information

© Springer Japan 2014

Authors and Affiliations

  • Chuan-Feng Huang
    • 1
    • 2
  • Cheng-Chao Sun
    • 1
  • Fang Zhao
    • 3
  • Ya-Dong Zhang
    • 4
  • De-Jia Li
    • 1
  1. 1.Department of Occupational and Environmental Health, School of Public HealthWuhan UniversityWuhanChina
  2. 2.Department of Pharmacology, Basic Medical SchoolNanyang Medical CollegeNanyangChina
  3. 3.Department of Cardiovascular MedicineThe Affiliated Zhongnan Hospital of Wuhan UniversityWuhanChina
  4. 4.Central Laboratory, The Central Hospital of Wuhan, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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