Journal of Gastroenterology

, Volume 48, Issue 1, pp 125–131

IL-23R polymorphisms, HBV infection, and risk of hepatocellular carcinoma in a high-risk Chinese population

  • Yan Xu
  • Yao Liu
  • Shandong Pan
  • Li Liu
  • Jibin Liu
  • Xiangjun Zhai
  • Hongbing Shen
  • Zhibin Hu
Original Article—Liver, Pancreas, and Biliary Tract
  • 536 Downloads

Abstract

Background

The interleukin-23 receptor (IL-23R) plays an important role in the T-helper 17 cell-mediated inflammatory process and is also involved in tumor immune surveillance, which may be linked to carcinogenesis in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). In this study, we hypothesized that potentially functional genetic variants of the IL-23R gene may modify HCC risk.

Methods

We genotyped two single-nucleotide polymorphisms (SNPs) of IL-23R, rs6682925 and rs1884444, in a case–control study of 837 HCC cases, 899 HBV surface antigen (HBsAg)-positive controls, and 743 HBsAg-negative controls. A reporter gene assay was performed to evaluate the functional relevance of the rs6682925 SNP located at the promoter region of the IL-23R gene.

Results

We found that the two SNPs were associated with the risk of HCC when compared with both the HBsAg-positive and -negative controls. When compared with all controls, IL-23R rs6682925 and rs1884444 both increased the HCC risk in a recessive genetic model [rs6682925 CC vs. TT/TC: odds ratio (OR) 1.35, 95 % confidence interval (CI) 1.07–1.70; rs1884444 GG vs. TT/TG: OR 1.36, 95 % CI 1.05–1.77]. Furthermore, the variant C allele of rs6682925 in the promoter region of IL-23R was associated with increased reporter gene activity.

Conclusions

These findings indicate that genetic variants in IL-23R may contribute to HCC development.

Keywords

Interleukin-23 receptor Polymorphism Hepatocellular carcinoma HBV Reporter gene 

Supplementary material

535_2012_620_MOESM1_ESM.doc (56 kb)
Supplementary Tables 1–3 (DOC 55 kb)

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Copyright information

© Springer 2012

Authors and Affiliations

  • Yan Xu
    • 1
  • Yao Liu
    • 1
  • Shandong Pan
    • 1
  • Li Liu
    • 2
  • Jibin Liu
    • 2
  • Xiangjun Zhai
    • 3
  • Hongbing Shen
    • 1
    • 4
  • Zhibin Hu
    • 1
    • 4
  1. 1.Department of Epidemiology and Biostatistics, MOE Key Laboratory of Modern Toxicology, State Key Laboratory of Reproductive MedicineNanjing Medical UniversityNanjingChina
  2. 2.Department of Hepatobiliary SurgeryNantong Tumor HospitalNantongChina
  3. 3.Department of Infection DiseasesJiangsu Province Center for Disease Prevention and ControlNanjingChina
  4. 4.Section of Clinical Epidemiology, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer CenterNanjing Medical UniversityNanjingChina

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